UROC1 (NM_144639) Human Tagged ORF Clone

CAT#: RC218737

UROC1 (Myc-DDK-tagged)-Human urocanase domain containing 1 (UROC1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY408229) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218737 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified UROC1 protein (Cat# TP318737). The protein was produced from HEK293T cells transfected with UROC1 cDNA clone (Cat# RC218737) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: UROC1
• Synonyms: HMFN0320; UROCD
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218737 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTAGCCTCCAGGCGCTGTGCTCTGGCCTGCCCCTGCGGCCCCTCCCAGAGAACCGGGGACGCCAGG
  CTGGGGTGCCCCATGCCCCTGTCAGGACCCCCAGCCTCAGCCCTGTGGAGAAACAGCTGGCGCTGAGGAA
  CGCCCTGCGCTACTTCCCCCCGGATGTCCAGGAGCTGCTGGCCCCAGAGTTTGCCCAGGAGCTGCAACTG
  TACGGACACATCTACATGTACCGGTTTTGCCCCGACATTGAAATGAGGGCCTACCCGATTGAGCAGTACC
  CCTGCCAGACGAAAGTGGCTGCCGCCATCATGCACATGATTATGAACAACCTGGATCCTGCCGTGGCCCA
  GTTTCCCCAGGAGCTGGTGACCTATGGAGGAAATGGGCAGGTGTTCAGCAACTGGGCTCAGTTCTGGCTG
  ACCATGTTCTACTTGTCGAAGATGACAGAGGAGCAGACTTTGGTCATGTACAGTGGGCACCCACTTGGCC
  TCTTTCCCAGCAGCCGCAGTGCCCCACGGCTCGTCATCACCAATGGGATGGTCATTCCCAACTACTCCTC
  CCGGACGGAGTATGAGAAGCTCTTTGCCTTGGGGGTTACAATGTACGGCCAGATGACAGCAGGTAGCTAC
  TGCTACATCGGTCCCCAGGGAATCGTTCATGGCACTGTGCTCACCGTGTTGAATGCTGCACGTCGGTACC
  TGGGCATCGAGGACTTGGCTGGGAAGGTCTTTGTCACCTCTGGGCTCGGCGGAATGAGTGGGGCTCAGGC
  CAAGGCCGCAGTCATCGTGGGGTGCATCGGTGTGATAGCAGAGGTGGATAAAGCAGCCCTTGAGAAACGC
  CACAGGCAGGGCTGGCTGATGGAAGTGACTGACAGCTTGGACCGCTGCATCCAGAGGCTCAGGGAAGCAA
  GGAAAAAAAAGGAGGTGCTCAGCCTTGGTTACCATGGCAACGTGGTGGCTCTTTGGGAGCGCCTGGTCCA
  CGAATTGGACACGACGGGGGAGTGCTTGGTGGACCTGGGGTCAGATCAGACATCCTGCCACAACCCGTTC
  AATGGCGGCTACTACCCTGTGCAGCTCAGCTTCACGGAGGCCCAGAGCCTCATGGCCTCCAACCCTGCTG
  TGTTCAAGGACCTGGTCCAGGAAAGCCTGAGGAGGCAAGTCTCAGCCATCAACAGGTTGGCCGAGGAGAA
  GTTCTTCTTCTGGGACTACGGCAATGCCTTCCTCTTGGAGGCCCAGAGAGCAGGAGCGGATGTGGAGAAG
  AAAGGTGCTGGCAGGACAGAGTTCCGCTACCCTTCCTATGTGCAGCACATCATGGGGGACATATTCTCCC
  AGGGATTTGGGCCTTTCCGCTGGGTGTGCACATCGGGGGACCCCCAGGACCTGGCGGTCACAGACGAACT
  GGCCACATCTGTGCTGGAGGAAGCCATTGCTGATGGAGTGAAGGTGTCTGTGAAGCTGCAGTACATGGAC
  AACATCCGCTGGATCCGGGAGGCCGCCAGGCACCGGCTGGTGGTGGGCTCCCAGGCAAGGATCCTGTACT
  CAGACCAGAAGGGCCGCGTGGCCATCGCTGTGGCCATTAACCAGGCCATCGCCTGCAGGAGGATCAAGGC
  GCCGGTGGTCCTGAGCCGAGATCACCATGACGTGAGCGGCACCGACAGCCCCTTTAGGGAGACCTCCAAC
  ATTTACGACGGCTCTGCCTTCTGTGCAGACATGGCTGTGCAGAACTTCGTGGGAGATGCCTGTCGCGGAG
  CCACCTGGGTCGCCCTTCACAACGGAGGGGGCGTGGGCTGGGGTGAGGTGATCAACGGGGGATTCGGCCT
  CGTGCTGGACGGTACCCCGGAGGCTGAGGGGAGAGCCAGGCTGATGCTCAGCTGGGATGTCTCCAATGGT
  GTGGCCCGGCGCTGCTGGTCAGGGAACCAGAAGGCCTATGAGATCATCTGCCAGACCATGCAGGAGAACA
  GCACCTTGGTGGTGACACTGCCTCACAAGGTGGAGGACGAGCGGGTGCTCCAGCAGGCCCTGCAGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218737 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MSSLQALCSGLPLRPLPENRGRQAGVPHAPVRTPSLSPVEKQLALRNALRYFPPDVQELLAPEFAQELQL
  YGHIYMYRFCPDIEMRAYPIEQYPCQTKVAAAIMHMIMNNLDPAVAQFPQELVTYGGNGQVFSNWAQFWL
  TMFYLSKMTEEQTLVMYSGHPLGLFPSSRSAPRLVITNGMVIPNYSSRTEYEKLFALGVTMYGQMTAGSY
  CYIGPQGIVHGTVLTVLNAARRYLGIEDLAGKVFVTSGLGGMSGAQAKAAVIVGCIGVIAEVDKAALEKR
  HRQGWLMEVTDSLDRCIQRLREARKKKEVLSLGYHGNVVALWERLVHELDTTGECLVDLGSDQTSCHNPF
  NGGYYPVQLSFTEAQSLMASNPAVFKDLVQESLRRQVSAINRLAEEKFFFWDYGNAFLLEAQRAGADVEK
  KGAGRTEFRYPSYVQHIMGDIFSQGFGPFRWVCTSGDPQDLAVTDELATSVLEEAIADGVKVSVKLQYMD
  NIRWIREAARHRLVVGSQARILYSDQKGRVAIAVAINQAIACRRIKAPVVLSRDHHDVSGTDSPFRETSN
  IYDGSAFCADMAVQNFVGDACRGATWVALHNGGGVGWGEVINGGFGLVLDGTPEAEGRARLMLSWDVSNG
  VARRCWSGNQKAYEIICQTMQENSTLVVTLPHKVEDERVLQQALQL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144639
• ORF Size: 2028 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144639.1, NM_144639.2, NP_653240.1
• RefSeq Size: 3280 bp
• RefSeq ORF: 2031 bp
• Locus ID: 131669
• Protein Pathways: Histidine metabolism, Metabolic pathways
• MW: 74.8 kDa
• Gene Summary: This gene encodes an enzyme involved in histidine catabolism, metabolizing urocanic acid to formiminoglutamic acid. The gene product is known to protect the skin from ultra violet rays and is contained in human sweat. Deficiency of this gene product in the liver is an apparent cause of cognitive disability. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Sep 2009]