SUPT16H (NM_007192) Human Tagged ORF Clone

CAT#: RC218797

SUPT16H (Myc-DDK-tagged)-Human suppressor of Ty 16 homolog (S. cerevisiae) (SUPT16H)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SUPT16H
• Synonyms: CDC68; FACTP140; SPT16; SPT16/CDC68
• Vector: pCMV6-AC-Myc-DDK
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218797 representing NM_007192
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTGTGACTCTGGACAAAGACGCTTATTATCGGCGAGTGAAGAGACTGTACAGCAATTGGCGGAAAG
  GAGAAGATGAGTATGCCAACGTTGATGCCATTGTTGTATCAGTGGGTGTTGATGAAGAAATTGTTTATGC
  CAAATCAACTGCCTTACAGACATGGCTCTTTGGTTATGAACTAACTGATACTATCATGGTCTTTTGTGAT
  GACAAAATCATCTTTATGGCCAGCAAGAAAAAAGTGGAGTTCTTGAAACAGATTGCCAACACTAAGGGCA
  ATGAGAATGCTAATGGAGCCCCTGCCATCACACTGCTAATACGAGAAAAGAATGAAAGTAATAAGAGTAG
  CTTTGACAAAATGATTGAAGCCATTAAAGAAAGCAAGAATGGCAAGAAGATTGGAGTGTTCAGCAAAGAC
  AAATTCCCTGGAGAGTTCATGAAGAGCTGGAATGACTGCCTCAACAAAGAAGGCTTTGACAAAATAGATA
  TCAGTGCAGTTGTGGCATATACCATCGCTGTAAAGGAGGATGGGGAGCTCAACCTAATGAAGAAAGCAGC
  CAGCATCACTTCTGAAGTCTTCAACAAATTCTTCAAGGAAAGAGTCATGGAAATAGTTGATGCAGATGAG
  AAAGTTCGACACAGCAAACTGGCTGAGTCTGTGGAAAAGGCCATTGAAGAGAAAAAATACCTTGCTGGGG
  CAGACCCTTCTACTGTGGAAATGTGTTACCCTCCTATCATTCAGAGTGGTGGCAACTATAATCTCAAGTT
  CAGTGTGGTGAGTGACAAGAATCATATGCACTTTGGGGCTATCACTTGTGCCATGGGTATTCGCTTCAAG
  TCTTACTGCTCCAACCTTGTTCGCACTTTGATGGTTGATCCTTCTCAAGAAGTTCAAGAAAATTATAACT
  TTTTGCTCCAGCTTCAAGAGGAGCTGCTGAAGGAATTAAGACATGGTGTGAAGATATGTGACGTGTATAA
  CGCTGTCATGGACGTGGTTAAAAAGCAGAAGCCAGAACTGCTGAACAAAATTACCAAAAACCTAGGGTTT
  GGGATGGGAATTGAATTCCGTGAAGGCTCCCTAGTAATCAATAGCAAAAATCAATACAAACTGAAGAAAG
  GAATGGTTTTCAGCATCAATTTAGGATTCTCAGACCTGACTAACAAGGAGGGGAAAAAGCCAGAAGAGAA
  AACCTATGCCCTGTTCATTGGTGACACAGTGCTTGTGGATGAGGATGGCCCAGCTACTGTTCTCACTTCT
  GTGAAGAAGAAAGTGAAGAATGTGGGGATTTTCCTAAAGAATGAAGATGAGGAAGAAGAGGAGGAGGAGA
  AAGATGAGGCAGAGGACCTTTTGGGAAGAGGTTCTCGGGCAGCATTACTTACAGAAAGAACAAGAAATGA
  AATGACTGCAGAAGAGAAGCGAAGAGCACATCAGAAAGAACTAGCGGCTCAACTCAATGAAGAAGCAAAG
  AGGCGATTGACTGAACAAAAGGGAGAACAGCAGATTCAGAAAGCTCGCAAGTCTAATGTGTCCTATAAAA
  ACCCATCTCTGATGCCTAAGGAACCGCATATTCGGGAAATGAAGATCTACATCGATAAGAAATATGAGAC
  TGTAATAATGCCCGTGTTTGGCATTGCAACACCGTTTCACATTGCCACAATCAAGAATATAAGTATGTCC
  GTGGAAGGAGATTATACTTACTTGCGAATCAACTTTTATTGCCCAGGCAGTGCTCTGGGCAGGAATGAAG
  GCAACATCTTTCCTAACCCTGAAGCGACTTTTGTCAAGGAAATTACATACCGAGCATCAAATATTAAGGC
  ACCCGGAGAACAGACAGTACCAGCCTTGAACCTTCAGAATGCTTTCCGAATTATTAAAGAAGTACAGAAA
  CGTTATAAAACTCGAGAAGCTGAAGAGAAAGAGAAGGAGGGGATTGTAAAACAAGACTCACTGGTGATCA
  ATCTAAACCGGAGTAATCCGAAACTGAAAGATCTATACATTCGCCCAAATATTGCCCAAAAGAGGATGCA
  AGGCTCACTGGAGGCCCATGTCAATGGCTTCCGCTTCACATCTGTTCGAGGAGACAAAGTGGATATTTTG
  TACAATAATATTAAGCATGCTTTGTTCCAGCCCTGTGATGGAGAAATGATTATTGTCTTGCACTTTCACC
  TCAAGAATGCCATCATGTTTGGGAAGAAGCGGCACACGGATGTGCAGTTCTACACAGAAGTGGGAGAGAT
  AACCACGGACTTGGGGAAACATCAGCATATGCATGACCGAGATGACCTCTATGCTGAGCAGATGGAACGA
  GAAATGAGGCACAAACTGAAAACAGCCTTTAAAAATTTCATTGAGAAAGTAGAGGCTCTAACTAAGGAGG
  AACTGGAATTTGAAGTGCCTTTTAGGGACTTGGGATTTAACGGAGCTCCCTATAGGAGTACCTGCCTCCT
  TCAGCCCACTAGTAGTGCGCTGGTAAATGCTACGGAATGGCCACCTTTTGTGGTGACATTGGATGAGGTA
  GAGCTGATCCACTTTGAGCGGGTCCAGTTTCACCTGAAGAACTTTGATATGGTAATCGTCTACAAGGACT
  ACAGCAAGAAAGTGACCATGATCAACGCCATTCCTGTAGCCTCTCTTGACCCCATCAAGGAATGGTTGAA
  TTCCTGCGACCTGAAATACACAGAAGGAGTACAGTCCCTCAACTGGACTAAAATCATGAAGACCATTGTT
  GATGACCCTGAGGGCTTCTTCGAACAAGGTGGCTGGTCTTTCCTGGAGCCTGAGGGTGAGGGGAGTGATG
  CTGAAGAAGGGGATTCAGAGTCTGAAATTGAAGATGAGACTTTTAATCCTTCAGAAGATGACTATGAAGA
  GGAAGAGGAGGACAGTGATGAAGATTATTCATCAGAAGCAGAAGAGTCAGACTATTCTAAGGAGTCATTG
  GGTAGTGAAGAAGAGAGTGGAAAGGATTGGGATGAACTGGAGGAAGAAGCCCGAAAAGCGGACCGAGAAA
  GTCGTTACGAGGAAGAAGAAGAACAAAGTCGAAGTATGAGCCGGAAGAGGAAGGCATCTGTGCACAGTTC
  GGGCCGTGGCTCTAACCGTGGTTCCAGACACAGCTCTGCACCCCCCAAGAAAAAGAGGAAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218797 representing NM_007192
  Red=Cloning site Green=Tags(s)
  
  MAVTLDKDAYYRRVKRLYSNWRKGEDEYANVDAIVVSVGVDEEIVYAKSTALQTWLFGYELTDTIMVFCD
  DKIIFMASKKKVEFLKQIANTKGNENANGAPAITLLIREKNESNKSSFDKMIEAIKESKNGKKIGVFSKD
  KFPGEFMKSWNDCLNKEGFDKIDISAVVAYTIAVKEDGELNLMKKAASITSEVFNKFFKERVMEIVDADE
  KVRHSKLAESVEKAIEEKKYLAGADPSTVEMCYPPIIQSGGNYNLKFSVVSDKNHMHFGAITCAMGIRFK
  SYCSNLVRTLMVDPSQEVQENYNFLLQLQEELLKELRHGVKICDVYNAVMDVVKKQKPELLNKITKNLGF
  GMGIEFREGSLVINSKNQYKLKKGMVFSINLGFSDLTNKEGKKPEEKTYALFIGDTVLVDEDGPATVLTS
  VKKKVKNVGIFLKNEDEEEEEEEKDEAEDLLGRGSRAALLTERTRNEMTAEEKRRAHQKELAAQLNEEAK
  RRLTEQKGEQQIQKARKSNVSYKNPSLMPKEPHIREMKIYIDKKYETVIMPVFGIATPFHIATIKNISMS
  VEGDYTYLRINFYCPGSALGRNEGNIFPNPEATFVKEITYRASNIKAPGEQTVPALNLQNAFRIIKEVQK
  RYKTREAEEKEKEGIVKQDSLVINLNRSNPKLKDLYIRPNIAQKRMQGSLEAHVNGFRFTSVRGDKVDIL
  YNNIKHALFQPCDGEMIIVLHFHLKNAIMFGKKRHTDVQFYTEVGEITTDLGKHQHMHDRDDLYAEQMER
  EMRHKLKTAFKNFIEKVEALTKEELEFEVPFRDLGFNGAPYRSTCLLQPTSSALVNATEWPPFVVTLDEV
  ELIHFERVQFHLKNFDMVIVYKDYSKKVTMINAIPVASLDPIKEWLNSCDLKYTEGVQSLNWTKIMKTIV
  DDPEGFFEQGGWSFLEPEGEGSDAEEGDSESEIEDETFNPSEDDYEEEEEDSDEDYSSEAEESDYSKESL
  GSEEESGKDWDELEEEARKADRESRYEEEEEQSRSMSRKRKASVHSSGRGSNRGSRHSSAPPKKKRK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_007192
• ORF Size: 3141 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_007192.1, NM_007192.2, NM_007192.3, NP_009123.1
• RefSeq Size: 4696
• RefSeq ORF: 3144
• Locus ID: 11198
• Domains: Peptidase_M24
• Protein Families: Druggable Genome, Transcription Factors
• MW: 119.7 kDa
• Gene Summary: Transcription of protein-coding genes can be reconstituted on naked DNA with only the general transcription factors and RNA polymerase II. However, this minimal system cannot transcribe DNA packaged into chromatin, indicating that accessory factors may facilitate access to DNA. One such factor, FACT (facilitates chromatin transcription), interacts specifically with histones H2A/H2B to effect nucleosome disassembly and transcription elongation. FACT is composed of an 80 kDa subunit and a 140 kDa subunit; this gene encodes the 140 kDa subunit. [provided by RefSeq, Feb 2009]