BCL9L (NM_182557) Human Tagged ORF Clone

CAT#: RC218806

BCL9L (Myc-DDK-tagged)-Human B-cell CLL/lymphoma 9-like (BCL9L)

Product Images

Western blot validation of overexpression lysate (Cat# LY405481) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218806 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: BCL9L
• Synonyms: B9L; BCL9-2; DLNB11
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218806 representing NM_182557
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGGATCCTGGCTAACAAGACAAGGTTACCCCACCCCAGGAGGAGAGAAGCTCCAGGGAGCCCGCCGC
  TGTCCCCCCGCGGTCATTGCCCCCCTGCCCCAGCCAAGCCAATGCACCCAGAAAATAAATTGACCAATCA
  TGGCAAGACAGGGAATGGCGGGGCCCAATCTCAGCACCAGAATGTGAACCAAGGACCCACCTGCAACGTG
  GGCTCGAAGGGCGTGGGGGCGGGGAACCATGGGGCCAAGGCCAACCAGATCTCGCCTAGCAACTCAAGTC
  TGAAGAACCCCCAGGCAGGGGTGCCCCCTTTCAGCTCGCTCAAGGGCAAGGTGAAGAGGGACCGGAGTGT
  GTCTGTGGACTCTGGAGAGCAGCGAGAGGCTGGGACCCCATCCCTGGATTCAGAGGCCAAAGAGGTGGCG
  CCGCGGAGTAAGCGGCGCTGTGTGCTGGAGCGGAAGCAGCCGTACAGTGGGGACGAATGGTGCTCTGGAC
  CGGACAGTGAGGAGGACGACAAGCCCATTGGGGCCACCCACAATTGTAATGTAGCAGACCCAGCCATGGC
  GGCCCCACAGCTGGGTCCCGGCCAAACCACCCAACTGCCCCTCAGCGAGAGCAGCGTGCCAGGCGCCCCG
  CACGGCCCTCCTCCTGGCCTTCGGCCTGATGCCCCTGGGGGCGGGGGCGGGGGCGGGGGCGTCCCCGGAA
  AGCCTCCCTCGCAGTTCGTATATGTCTTCACCACCCACCTGGCCAACACGGCTGCAGAGGCAGTGCTGCA
  GGGCCGGGCCGACTCCATCCTCGCCTACCACCAGCAGAACGTGCCCCGGGCCAAGCTTGACCAGGCCCCT
  AAAGTGCCCCCCACCCCAGAACCGCTACCCCTGAGCACGCCGTCAGCAGGCACCCCGCAGTCCCAGCCAC
  CTCCACTGCCGCCGCCGCCACCCCCGGCCCCTGGCAGTGCCCCGCCTGCTCTGCCCCCAGAGGGGCCTCC
  TGAGGACAGCAGTCAGGACCTGGCCCCCAACTCGGTGGGAGCTGCCAGCACAGGTGGTGGGACTGGGGGC
  ACCCACCCTAACACCCCGACGGCTACCACCGCCAACAACCCTCTGCCTCCTGGAGGAGACCCCAGCAGTG
  CCCCCGGCCCTGCCCTGCTGGGGGAGGCAGCCGCCCCTGGAAATGGGCAGCGCAGCCTGGTGGGCTCAGA
  GGGCTTGTCCAAAGAGCAGCTGGAGCATCGGGAACGGTCCCTCCAGACGCTGCGAGACATTGAGCGACTG
  CTGCTCCGCAGCGGAGAGACTGAGCCCTTCCTCAAGGGGCCCCCAGGAGGAGCGGGTGAGGGGGGCCCAC
  CAGCACAAGCCCCCCCTCCCCCCCAGCAGCCACCCACGGCCCCTCCCAGCGGGCTGAAGAAATATGAGGA
  ACCCTTGCAGTCCATGATTTCACAGACACAGAGCCTAGGGGGCCCCCCGCTGGAGCATGAAGTGCCTGGG
  CACCCCCCGGGTGGGGACATGGGGCAGCAGATGAACATGATGATACAGAGGCTGGGCCAGGACAGCCTCA
  CGCCTGAGCAGGTGGCCTGGCGCAAGCTGCAGGAGGAGTACTACGAAGAGAAACGGCGGAAAGAGGAACA
  GATTGGGCTGCATGGGAGCCGTCCTCTGCAGGACATGATGGGCATGGGGGGCATGATGGTGAGGGGGCCC
  CCGCCTCCTTACCACAGCAAGCCTGGGGATCAGTGGCCACCTGGAATGGGTGCGCAGCTGCGGGGGCCCA
  TGGATGTTCAAGATCCCATGCAGCTCCGGGGCGGACCTCCCTTTCCTGGGCCCCGTTTCCCAGGCAACCA
  GATACAACGGGTGCCTGGGTTTGGGGGCATGCAGAGTATGCCCATGGAGGTGCCCATGAATGCCATGCAG
  AGGCCCGTGAGACCAGGCATGGGCTGGACCGAAGACTTGCCCCCTATGGGGGGACCCAGCAATTTTGCCC
  AGAACACCATGCCCTACCCAGGTGGGCAGGGTGAGGCGGAGCGATTCATGACTCCCCGGGTCCGTGAGGA
  GCTGCTGCGGCACCAGCTGCTGGAGAAGCGGTCGATGGGCATGCAGCGCCCCCTGGGCATGGCAGGCAGT
  GGCATGGGACAGAGCATGGAGATGGAGCGGATGATGCAGGCGCACCGACAGATGGATCCTGCCATGTTTC
  CCGGGCAGATGGCTGGTGGTGAGGGCCTGGCGGGCACTCCCATGGGCATGGAGTTTGGTGGAGGCCGGGG
  CCTCCTGAGCCCTCCCATGGGGCAGTCTGGGCTGAGGGAGGTGGACCCACCCATGGGGCCAGGCAACCTC
  AACATGAACATGAATGTCAACATGAACATGAACATGAACCTGAACGTGCAGATGACCCCGCAGCAGCAGA
  TGCTGATGTCGCAGAAGATGCGGGGCCCTGGGGACTTGATGGGGCCCCAGGGCCTCAGTCCTGAGGAGAT
  GGCCCGGGTTCGGGCCCAGAACAGCAGTGGCGTGATGGGCGGCCCGCAGAAGATGCTGATGCCTTCACAG
  TTTCCCAACCAGGGCCAGCAGGGATTCTCTGGAGGCCAGGGACCCTACCAAGCCATGTCCCAGGACATGG
  GCAATACCCAAGACATGTTCAGCCCTGATCAGAGCTCAATGCCCATGAGCAACGTGGGCACCACCCGGCT
  CAGCCACATGCCTCTGCCCCCTGCGTCCAATCCTCCTGGGACCGTGCATTCAGCCCCAAACCGGGGGCTA
  GGCAGGCGGCCTTCGGACCTCACCATCAGTATTAATCAGATGGGCTCACCGGGCATGGGGCACTTGAAGT
  CGCCCACCCTTAGCCAGGTGCACTCACCCCTGGTCACCTCGCCCTCTGCCAACCTCAAGTCACCCCAGAC
  TCCCTCACAGATGGTGCCCTTGCCTTCTGCCAACCCGCCAGGACCTCTCAAGTCGCCCCAGGTCCTCGGC
  TCCTCCCTCAGTGTCCGTTCACCCACTGGCTCGCCCAGCAGGCTCAAGTCTCCTTCCATGGCGGTGCCTT
  CTCCAGGCTGGGTTGCCTCACCTAAGACGGCCATGCCCAGCCCGGGGGTCTCCCAGAACAAGCAGCCGCC
  TCTCAACATGAACTCTTCCACCACCCTGAGCAACATGGAACAGGGTACCCTCCCGCCTAGCGGCCCCCGG
  AGCAGCTCCTCAGCACCTCCCGCCAACCCTCCCAGCGGCCTCATGAACCCCAGCCTACCATTCACTTCCT
  CCCCAGACCCCACACCTTCCCAGAACCCCCTGTCACTGATGATGACCCAGATGTCCAAGTACGCCATGCC
  CAGCTCCACCCCGCTCTACCACAATGCCATCAAGACCATCGCCACCTCAGACGACGAGCTGCTGCCCGAC
  CGGCCCCTGCTGCCCCCCCCACCACCACCGCAGGGCTCCGGGCCAGGGATCAGCAACAGCCAGCCCAGCC
  AGATGCACCTGAACTCAGCCGCTGCCCAGAGCCCTATGGGCATGAACCTGCCAGGCCAGCAGCCCCTGTC
  CCATGAGCCCCCGCCCGCCATGCTGCCCTCCCCCACCCCTCTGGGCTCCAACATTCCACTGCATCCCAAC
  GCACAGGGGACAGGGGGGCCCCCTCAAAACTCCATGATGATGGCCCCAGGGGGCCCCGACTCCCTGAATG
  CCCCCTGTGGCCCAGTGCCCAGCTCCTCCCAGATGATGCCCTTCCCCCCTCGGCTGCAGCAGCCCCATGG
  TGCCATGGCCCCCACTGGGGGTGGGGGCGGGGGGCCTGGCCTGCAGCAGCACTACCCGTCAGGCATGGCC
  CTGCCTCCCGAGGACCTGCCCAACCAGCCGCCAGGCCCCATGCCTCCCCAGCAGCACCTGATGGGCAAAG
  CCATGGCTGGGCGCATGGGCGACGCATACCCACCGGGTGTGCTCCCTGGGGTGGCATCAGTGCTGAACGA
  CCCCGAGCTGAGCGAGGTGATCCGGCCCACCCCAACGGGGATCCCCGAGTTCGACTTGTCGAGGATCATC
  CCCTCTGAGAAGCCAAGCAGCACCCTCCAGTACTTCCCCAAGAGCGAGAACCAGCCCCCCAAGGCTCAGC
  CCCCTAATCTGCATCTCATGAACCTGCAGAACATGATGGCGGAGCAGACTCCCTCTCGGCCTCCCAACCT
  CCCAGGCCAGCAGGGCGTCCAGCGGGGGCTCAACATGTCCATGTGCCACCCCGGACAGATGTCCTTGCTG
  GGCAGGACAGGCGTGCCCCCACAGCAGGGGATGGTGCCCCATGGCCTGCACCAGGGGGTCATGTCCCCTC
  CACAAGGCCTCATGACCCAGCAGAATTTCATGCTGATGAAGCAGCGGGGCGTGGGGGGCGAGGTCTACAG
  CCAGCCGCCCCACATGCTCTCCCCGCAGGGCTCCCTCATGGGCCCCCCGCCCCAGCAGAACCTCATGGTG
  TCCCACCCCCTTCGGCAGCGCAGTGTGTCCCTGGACAGCCAGATGGGCTACCTCCCGGCACCAGGCGGCA
  TGGCCAACCTGCCCTTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218806 representing NM_182557
  Red=Cloning site Green=Tags(s)
  
  MRILANKTRLPHPRRREAPGSPPLSPRGHCPPAPAKPMHPENKLTNHGKTGNGGAQSQHQNVNQGPTCNV
  GSKGVGAGNHGAKANQISPSNSSLKNPQAGVPPFSSLKGKVKRDRSVSVDSGEQREAGTPSLDSEAKEVA
  PRSKRRCVLERKQPYSGDEWCSGPDSEEDDKPIGATHNCNVADPAMAAPQLGPGQTTQLPLSESSVPGAP
  HGPPPGLRPDAPGGGGGGGGVPGKPPSQFVYVFTTHLANTAAEAVLQGRADSILAYHQQNVPRAKLDQAP
  KVPPTPEPLPLSTPSAGTPQSQPPPLPPPPPPAPGSAPPALPPEGPPEDSSQDLAPNSVGAASTGGGTGG
  THPNTPTATTANNPLPPGGDPSSAPGPALLGEAAAPGNGQRSLVGSEGLSKEQLEHRERSLQTLRDIERL
  LLRSGETEPFLKGPPGGAGEGGPPAQAPPPPQQPPTAPPSGLKKYEEPLQSMISQTQSLGGPPLEHEVPG
  HPPGGDMGQQMNMMIQRLGQDSLTPEQVAWRKLQEEYYEEKRRKEEQIGLHGSRPLQDMMGMGGMMVRGP
  PPPYHSKPGDQWPPGMGAQLRGPMDVQDPMQLRGGPPFPGPRFPGNQIQRVPGFGGMQSMPMEVPMNAMQ
  RPVRPGMGWTEDLPPMGGPSNFAQNTMPYPGGQGEAERFMTPRVREELLRHQLLEKRSMGMQRPLGMAGS
  GMGQSMEMERMMQAHRQMDPAMFPGQMAGGEGLAGTPMGMEFGGGRGLLSPPMGQSGLREVDPPMGPGNL
  NMNMNVNMNMNMNLNVQMTPQQQMLMSQKMRGPGDLMGPQGLSPEEMARVRAQNSSGVMGGPQKMLMPSQ
  FPNQGQQGFSGGQGPYQAMSQDMGNTQDMFSPDQSSMPMSNVGTTRLSHMPLPPASNPPGTVHSAPNRGL
  GRRPSDLTISINQMGSPGMGHLKSPTLSQVHSPLVTSPSANLKSPQTPSQMVPLPSANPPGPLKSPQVLG
  SSLSVRSPTGSPSRLKSPSMAVPSPGWVASPKTAMPSPGVSQNKQPPLNMNSSTTLSNMEQGTLPPSGPR
  SSSSAPPANPPSGLMNPSLPFTSSPDPTPSQNPLSLMMTQMSKYAMPSSTPLYHNAIKTIATSDDELLPD
  RPLLPPPPPPQGSGPGISNSQPSQMHLNSAAAQSPMGMNLPGQQPLSHEPPPAMLPSPTPLGSNIPLHPN
  AQGTGGPPQNSMMMAPGGPDSLNAPCGPVPSSSQMMPFPPRLQQPHGAMAPTGGGGGGPGLQQHYPSGMA
  LPPEDLPNQPPGPMPPQQHLMGKAMAGRMGDAYPPGVLPGVASVLNDPELSEVIRPTPTGIPEFDLSRII
  PSEKPSSTLQYFPKSENQPPKAQPPNLHLMNLQNMMAEQTPSRPPNLPGQQGVQRGLNMSMCHPGQMSLL
  GRTGVPPQQGMVPHGLHQGVMSPPQGLMTQQNFMLMKQRGVGGEVYSQPPHMLSPQGSLMGPPPQQNLMV
  SHPLRQRSVSLDSQMGYLPAPGGMANLPF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_182557
• ORF Size: 4497 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_182557.1, NM_182557.2, NP_872363.1
• RefSeq Size: 7539 bp
• RefSeq ORF: 4500 bp
• Locus ID: 283149
• MW: 156.9 kDa