STT3B (NM_178862) Human Tagged ORF Clone

CAT#: RC218829

STT3B (Myc-DDK-tagged)-Human STT3, subunit of the oligosaccharyltransferase complex, homolog B (S. cerevisiae) (STT3B). Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: STT3B
• Synonyms: CDG1X; SIMP; STT3-B
• Vector: pCMV6-AC-Myc-DDK
• E. coli Selection: Ampicillin (100 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218829 ORF sequence, codon optimized.
  Due to the complexity of NM_178862, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGAGCCCTCGGCCCCGGAGAGCAAGCACAAGTCGTCCCTCAACTCGTCCCCGTGGAGTGGCCTCA
  TGGCCCTGGGAAACAGCCGGCACGGCCACCACGGGCCCGGGGCCCAGTGCGCGCACAAGGCGGCGGGCGG
  CGCGGCGCCGCCGAAGCCGGCCCCGGCGGGGCTGTCCGGGGGGCTGTCGCAGCCGGCTGGGTGGCAGTCG
  CTTCTCTCCTTCACCATCCTCTTCCTGGCCTGGCTTGCCGGCTTCAGCTCGCGCCTCTTCGCCGTCATCC
  GCTTCGAAAGCATCATCCACGAGTTCGACCCGTGGTTTAACTATAGATCAACACATCATCTTGCATCTCA
  TGGGTTCTATGAATTTTTAAATTGGTTTGATGAAAGAGCATGGTATCCACTAGGAAGAATAGTAGGTGGT
  ACTGTTTACCCAGGGTTGATGATAACCGCTGGCCTTATTCATTGGATTTTAAATACATTGAACATAACTG
  TTCACATAAGAGACGTATGTGTGTTCCTTGCACCAACTTTTAGCGGCCTTACATCTATATCTACTTTCCT
  GCTTACAAGAGAACTTTGGAACCAAGGAGCAGGACTTTTAGCTGCTTGTTTTATTGCTATTGTACCAGGC
  TACATATCTCGGTCAGTAGCTGGATCCTTTGATAATGAAGGCATTGCTATTTTTGCACTTCAGTTCACAT
  ACTATTTATGGGTAAAATCTGTAAAAACTGGGTCAGTTTTTTGGACAATGTGCTGCTGCTTATCCTATTT
  CTATATGGTCTCTGCTTGGGGTGGTTATGTATTTATCATCAATCTTATTCCACTGCATGTATTTGTGTTG
  TTACTGATGCAGAGATACAGCAAAAGAGTCTACATAGCATATAGCACTTTCTACATTGTGGGTTTAATAT
  TATCAATGCAGATACCTTTTGTGGGATTCCAGCCAATCAGAACAAGTGAACACATGGCAGCTGCAGGTGT
  CTTTGCATTGCTGCAAGCTTATGCTTTCTTGCAGTATCTGAGAGACCGATTAACAAAACAAGAGTTCCAG
  ACCCTTTTCTTTTTGGGTGTATCACTAGCTGCAGGTGCTGTGTTCCTTAGTGTCATCTATTTGACTTATA
  CAGGTTACATTGCACCATGGAGTGGCAGGTTTTATTCATTGTGGGATACTGGGTATGCAAAAATACACAT
  TCCAATTATTGCATCAGTGTCTGAGCATCAACCTACGACTTGGGTGTCTTTCTTCTTTGATCTACATATT
  CTCGTTTGTACTTTTCCCGCAGGATTGTGGTTCTGTATTAAAAACATTAACGATGAGCGCGTATTTGTGG
  CACTGTACGCCATTAGCGCAGTCTATTTTGCTGGTGTGATGGTCCGCCTCATGCTGACATTGACGCCGGT
  CGTTTGCATGCTCTCAGCTATTGCCTTCTCTAATGTGTTTGAACATTATCTCGGAGATGACATGAAAAGA
  GAAAACCCCCCAGTTGAGGACTCTTCCGACGAGGATGACAAAAGAAACCAGGGTAACCTTTACGACAAGG
  CCGGCAAAGTTCGCAAGCATGCTACAGAGCAAGAAAAAACAGAGGAGGGGCTGGGGCCAAACATTAAATC
  AATAGTAACAATGCTGATGCTCATGCTGCTTATGATGTTCGCGGTGCACTGTACATGGGTGACATCTAAT
  GCTTATTCTTCTCCTTCAGTAGTCTTGGCATCCTATAACCATGATGGAACACGGAACATTCTTGATGACT
  TCCGCGAGGCTTACTTCTGGTTGCGGCAAAATACGGACGAACACGCTCGGGTGATGAGCTGGTGGGATTA
  TGGATATCAAATCGCGGGCATGGCTAATAGGACTACTCTTGTTGATAATAATACTTGGAACAACTCACAC
  ATTGCACTTGTCGGTAAGGCCATGAGTTCCAACGAGACGGCTGCATACAAAATCATGCGAACACTGGATG
  TTGATTACGTGCTGGTAATTTTTGGCGGAGTGATAGGATATTCTGGCGATGACATAAACAAGTTTCTGTG
  GATGGTAAGGATTGCCGAGGGGGAGCATCCCAAAGATATACGAGAAAGCGATTACTTTACCCCACAAGGA
  GAATTCCGCGTGGACAAAGCCGGCTCCCCTACTCTCTTGAATTGCCTGATGTACAAAATGAGCTATTATC
  GATTTGGCGAAATGCAGCTCGATTTCAGAACACCCCCAGGCTTTGATAGAACCCGGAATGCAGAGATAGG
  TAACAAAGATATTAAATTCAAGCACCTGGAAGAAGCGTTTACCTCTGAACACTGGTTGGTCCGAATTTAT
  AAAGTCAAAGCTCCCGATAATCGCGAAACTCTTGACCATAAACCACGGGTGACTAATATATTCCCAAAGC
  AGAAATACCTTTCCAAGAAAACAACAAAGAGGAAGAGAGGTTACATAAAAAATAAGCTCGTTTTCAAAAA
  GGGTAAGAAGATCAGTAAGAAAACAGTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218829 representing NM_178862
  Red=Cloning site Green=Tags(s)
  
  MAEPSAPESKHKSSLNSSPWSGLMALGNSRHGHHGPGAQCAHKAAGGAAPPKPAPAGLSGGLSQPAGWQS
  LLSFTILFLAWLAGFSSRLFAVIRFESIIHEFDPWFNYRSTHHLASHGFYEFLNWFDERAWYPLGRIVGG
  TVYPGLMITAGLIHWILNTLNITVHIRDVCVFLAPTFSGLTSISTFLLTRELWNQGAGLLAACFIAIVPG
  YISRSVAGSFDNEGIAIFALQFTYYLWVKSVKTGSVFWTMCCCLSYFYMVSAWGGYVFIINLIPLHVFVL
  LLMQRYSKRVYIAYSTFYIVGLILSMQIPFVGFQPIRTSEHMAAAGVFALLQAYAFLQYLRDRLTKQEFQ
  TLFFLGVSLAAGAVFLSVIYLTYTGYIAPWSGRFYSLWDTGYAKIHIPIIASVSEHQPTTWVSFFFDLHI
  LVCTFPAGLWFCIKNINDERVFVALYAISAVYFAGVMVRLMLTLTPVVCMLSAIAFSNVFEHYLGDDMKR
  ENPPVEDSSDEDDKRNQGNLYDKAGKVRKHATEQEKTEEGLGPNIKSIVTMLMLMLLMMFAVHCTWVTSN
  AYSSPSVVLASYNHDGTRNILDDFREAYFWLRQNTDEHARVMSWWDYGYQIAGMANRTTLVDNNTWNNSH
  IALVGKAMSSNETAAYKIMRTLDVDYVLVIFGGVIGYSGDDINKFLWMVRIAEGEHPKDIRESDYFTPQG
  EFRVDKAGSPTLLNCLMYKMSYYRFGEMQLDFRTPPGFDRTRNAEIGNKDIKFKHLEEAFTSEHWLVRIY
  KVKAPDNRETLDHKPRVTNIFPKQKYLSKKTTKRKRGYIKNKLVFKKGKKISKKTV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene
• ACCN: NM_178862
• ORF Size: 2478 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_178862.1, NM_178862.2, NP_849193.1
• RefSeq Size: 4553
• RefSeq ORF: 2481
• Locus ID: 201595
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, N-Glycan biosynthesis
• MW: 93.7 kDa
• Gene Summary: The protein encoded by this gene is a catalytic subunit of a protein complex that transfers oligosaccharides onto asparagine residues. Defects in this gene are a cause of congenital disorder of glycosylation Ix (CDG1X). [provided by RefSeq, Jun 2014]