Thromboxane A2 receptor (TBXA2R) (NM_001060) Human Tagged ORF Clone

CAT#: RC218915

TBXA2R (Myc-DDK-tagged)-Human thromboxane A2 receptor (TBXA2R), transcript variant a


  "NM_001060" in other vectors (6)

Reconstitution Protocol

USD 420.00

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Size
    • 10 ug

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Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Symbol TBXA2R
Synonyms BDPLT13; TXA2-R
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC218915 representing NM_001060
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGTGGCCCAACGGCAGTTCCCTGGGGCCCTGTTTCCGGCCCACAAACATTACCCTGGAGGAGAGACGGC
TGATCGCCTCGCCCTGGTTCGCCGCCTCCTTCTGCGTGGTGGGCCTGGCCTCCAACCTGCTGGCCCTGAG
CGTGCTGGCGGGCGCGCGGCAGGGGGGTTCGCACACGCGCTCCTCCTTCCTCACCTTCCTCTGCGGCCTC
GTCCTCACCGACTTCCTGGGGCTGCTGGTGACCGGTACCATCGTGGTGTCCCAGCACGCCGCGCTCTTCG
AGTGGCACTCCGTGGACCCTGGCTGCCGTCTCTGTCGCTTCATGGGCGTCGTCATGATCTTCTTCGGCCT
GTCCCCGCTGCTGCTGGGGGCCGCCATGGCCTCAGAGCGCTACCTGGGTATCACCCGGCCCTTCTCGCGC
CCGGCGGTCGCCTCGCAGCGCCGCGCCTGGGCCACCGTGGGGCTGGTGTGGGCGGCCGCGCTGGCGCTGG
GCCTGCTGCCCCTGCTGGGCGTGGGTCGCTACACCGTGCAATACCCGGGGTCCTGGTGCTTCCTGACGCT
GGGCGCCGAGTCCGGGGACGTGGCCTTCGGGCTGCTCTTCTCCATGCTGGGCGGCCTCTCGGTCGGGCTG
TCCTTCCTGCTGAACACGGTCAGCGTGGCCACCCTGTGCCACGTCTACCACGGGCAGGAGGCGGCCCAGC
AGCGTCCCCGGGACTCCGAGGTGGAGATGATGGCTCAGCTCCTGGGGATCATGGTGGTGGCCAGCGTGTG
TTGGCTGCCCCTTCTGGTCTTCATTGCCCAGACAGTGCTGCGAAACCCGCCTGCCATGAGCCCCGCCGGG
CAGCTGTCCCGCACCACGGAGAAGGAGCTGCTCATCTACTTGCGCGTGGCCACCTGGAACCAGATCCTGG
ACCCCTGGGTGTATATCCTGTTCCGCCGCGCCGTGCTCCGGCGTCTCCAGCCTCGCCTCAGCACCCGGCC
CAGGTCGCTGTCCCTCCAGCCCCAGCTCACGCAGCGCTCCGGGCTGCAG


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC218915 representing NM_001060
Red=Cloning site Green=Tags(s)

MWPNGSSLGPCFRPTNITLEERRLIASPWFAASFCVVGLASNLLALSVLAGARQGGSHTRSSFLTFLCGL
VLTDFLGLLVTGTIVVSQHAALFEWHSVDPGCRLCRFMGVVMIFFGLSPLLLGAAMASERYLGITRPFSR
PAVASQRRAWATVGLVWAAALALGLLPLLGVGRYTVQYPGSWCFLTLGAESGDVAFGLLFSMLGGLSVGL
SFLLNTVSVATLCHVYHGQEAAQQRPRDSEVEMMAQLLGIMVVASVCWLPLLVFIAQTVLRNPPAMSPAG
QLSRTTEKELLIYLRVATWNQILDPWVYILFRRAVLRRLQPRLSTRPRSLSLQPQLTQRSGLQ

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001060
ORF Size 1029 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001060.1, NM_001060.2, NM_001060.3, NM_001060.4, NM_001060.5, NP_001051.1
RefSeq Size 1414 bp
RefSeq ORF 1032 bp
Locus ID 6915
Cytogenetics 19p13.3
Domains 7tm_1
Protein Families Druggable Genome, GPCR, Transmembrane
Protein Pathways Calcium signaling pathway, Neuroactive ligand-receptor interaction
MW 37.3 kDa
Gene Summary 'This gene encodes a member of the G protein-coupled receptor family. The protein interacts with thromboxane A2 to induce platelet aggregation and regulate hemostasis. A mutation in this gene results in a bleeding disorder. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Oct 2009]'

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