CaMKK (CAMKK1) (NM_172206) Human Tagged ORF Clone

CAT#: RC218931

CAMKK1 (Myc-DDK-tagged)-Human calcium/calmodulin-dependent protein kinase kinase 1, alpha (CAMKK1), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY406737) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218931 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CAMKK1 protein (Cat# TP318931). The protein was produced from HEK293T cells transfected with CAMKK1 cDNA clone (Cat# RC218931) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CAMKK1
• Synonyms: CAMKKA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218931 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGGGGGGTCCAGCTGTCTGCTGCCAGGATCCTCGGGCAGAGCTGGTAGAACGGGTGGCAGCCATCG
  ATGTGACTCACTTGGAGGAGGCAGATGGTGGCCCAGAGCCTACTAGAAACGGTGTGGACCCCCCACCACG
  GGCCAGAGCTGCCTCTGTGATCCCTGGCAGTACTTCAAGACTGCTCCCAGCCCGGCCTAGCCTCTCAGCC
  AGGAAGCTTTCCCTACAGGAGCGGCCAGCAGGAAGCTATCTGGAGGCGCAGGCTGGGCCTTATGCCACGG
  GGCCTGCCAGCCACATCTCCCCCCGGGCCTGGCGGAGGCCCACCATCGAGTCCCACCACGTGGCCATCTC
  AGATGCAGAGGACTGCGTGCAGCTGAACCAGTACAAGCTGCAGAGTGAGATTGGCAAGGGTGCCTACGGT
  GTGGTGAGGCTGGCCTACAACGAAAGTGAAGACAGACACTATGCAATGAAAGTCCTTTCCAAAAAGAAGT
  TACTGAAGCAGTATGGCTTTCCACGTCGCCCTCCCCCGAGAGGGTCCCAGGCTGCCCAGGGAGGACCAGC
  CAAGCAGCTGCTGCCCCTGGAGCGGGTGTACCAGGAGATTGCCATCCTGAAGAAGCTGGACCACGTGAAT
  GTGGTCAAACTGATCGAGGTCCTGGATGACCCAGCTGAGGACAACCTCTATTTGGTGTTTGACCTCCTGA
  GAAAGGGGCCCGTCATGGAAGTGCCCTGTGACAAGCCCTTCTCGGAGGAGCAAGCTCGCCTCTACCTGCG
  GGACGTCATCCTGGGCCTCGAGTACTTGCACTGCCAGAAGATCGTCCACAGGGACATCAAGCCATCCAAC
  CTGCTCCTGGGGGATGATGGGCACGTGAAGATCGCCGACTTTGGCGTCAGCAACCAGTTTGAGGGGAACG
  ACGCTCAGCTGTCCAGCACGGCGGGAACCCCAGCATTCATGGCCCCCGAGGCCATTTCTGATTCCGGCCA
  GAGCTTCAGTGGGAAGGCCTTGGATGTATGGGCCACTGGCGTCACGTTGTACTGCTTTGTCTATGGGAAG
  TGCCCATTCATCGACGATTTCATCCTGGCCCTCCACAGGAAGATCAAGAATGAGCCCGTGGTGTTTCCTG
  AGGAGCCAGAAATCAGCGAGGAGCTCAAGGACCTGATCCTGAAGATGTTAGACAAGAATCCCGAGACGAG
  AATTGGGGTGCCAGACATCAAGTTGCACCCTTGGGTGACCAAGAACGGGGAGGAGCCCCTTCCTTCGGAG
  GAGGAGCACTGCAGCGTGGTGGAGGTGACAGAGGAGGAGGTTAAGAACTCAGTCAGGCTCATCCCCAGCT
  GGACCACGGTGATCCTGGTGAAGTCCATGCTGAGGAAGCGTTCCTTTGGGAACCCGTTTGAGCCCCAAGC
  ACGGAGGGAAGAGCGATCCATGTCTGCTCCAGGAAACCTACTGGTGAAAGAAGGGTTTGGTGAAGGGGGC
  AAGAGCCCAGAGCTCCCCGGCGTCCAGGAAGACGAGGCTGCATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218931 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MEGGPAVCCQDPRAELVERVAAIDVTHLEEADGGPEPTRNGVDPPPRARAASVIPGSTSRLLPARPSLSA
  RKLSLQERPAGSYLEAQAGPYATGPASHISPRAWRRPTIESHHVAISDAEDCVQLNQYKLQSEIGKGAYG
  VVRLAYNESEDRHYAMKVLSKKKLLKQYGFPRRPPPRGSQAAQGGPAKQLLPLERVYQEIAILKKLDHVN
  VVKLIEVLDDPAEDNLYLVFDLLRKGPVMEVPCDKPFSEEQARLYLRDVILGLEYLHCQKIVHRDIKPSN
  LLLGDDGHVKIADFGVSNQFEGNDAQLSSTAGTPAFMAPEAISDSGQSFSGKALDVWATGVTLYCFVYGK
  CPFIDDFILALHRKIKNEPVVFPEEPEISEELKDLILKMLDKNPETRIGVPDIKLHPWVTKNGEEPLPSE
  EEHCSVVEVTEEEVKNSVRLIPSWTTVILVKSMLRKRSFGNPFEPQARREERSMSAPGNLLVKEGFGEGG
  KSPELPGVQEDEAAS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172206
• ORF Size: 1515 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq Size: 3529 bp
• RefSeq ORF: 1599 bp
• Locus ID: 84254
• Protein Families: Druggable Genome, Protein Kinase
• Protein Pathways: Adipocytokine signaling pathway
• MW: 55.7 kDa
• Gene Summary: The product of this gene belongs to the Serine/Threonine protein kinase family, and to the Ca(2+)/calmodulin-dependent protein kinase subfamily. This protein plays a role in the calcium/calmodulin-dependent (CaM) kinase cascade. Three transcript variants encoding two distinct isoforms have been identified for this gene. [provided by RefSeq, Jul 2008]