ITGA8 (NM_003638) Human Tagged ORF Clone

CAT#: RC218936

ITGA8 (Myc-DDK-tagged)-Human integrin, alpha 8 (ITGA8)

Product Images

Western blot validation of overexpression lysate (Cat# LY418532) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC218936 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified ITGA8 protein (Cat# TP318936). The protein was produced from HEK293T cells transfected with ITGA8 cDNA clone (Cat# RC218936) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ITGA8
• Synonyms: integrin, alpha 8; OTTHUMP00000019207
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC218936 representing NM_003638
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGCCCGGGGCCAGCCGCGGTCCCCGGGGAAGCCAGGCGCCGCTGATCGCGCCCCTCTGCTGCGCCG
  CGGCCGCGCTGGGGATGTTGCTGTGGTCCCCCGCCTGTCAGGCGTTCAACCTGGACGTGGAAAAGCTCAC
  AGTGTACAGCGGCCCCAAGGGCAGCTACTTCGGCTACGCCGTGGACTTCCACATACCCGACGCCCGCACA
  GCGAGTGTCTTGGTGGGGGCGCCCAAAGCCAACACCAGCCAGCCCGATATCGTGGAAGGGGGAGCCGTCT
  ATTACTGTCCTTGGCCCGCGGAGGGGTCTGCGCAGTGCAGGCAGATACCGTTTGACACCACCAACAACAG
  AAAGATCAGAGTTAATGGAACCAAAGAACCTATCGAGTTCAAATCCAATCAGTGGTTTGGAGCAACAGTG
  AAAGCTCACAAAGGAAAAGTTGTGGCCTGTGCTCCTTTATATCACTGGAGAACTCTTAAACCGACACCAG
  AAAAGGACCCAGTTGGCACCTGCTATGTAGCAATTCAGAACTTCAGCGCCTATGCCGAGTTCTCTCCTTG
  CCGGAACAGCAATGCTGATCCGGAAGGCCAGGGTTACTGCCAAGCAGGATTTAGTCTGGATTTTTATAAG
  AATGGAGACCTTATTGTGGGAGGACCTGGGAGTTTCTACTGGCAAGGACAAGTGATCACTGCCAGTGTTG
  CAGATATCATTGCAAATTACTCATTCAAGGATATCCTCAGGAAACTGGCAGGAGAAAAGCAGACGGAAGT
  GGCTCCAGCTTCCTATGATGACAGTTACCTTGGATACTCAGTTGCTGCTGGGGAGTTTACTGGGGATTCT
  CAGCAAGAATTGGTTGCTGGAATTCCAAGAGGAGCACAGAATTTTGGATATGTTTCCATCATTAACTCTA
  CGGATATGACGTTTATTCAGAATTTCACGGGAGAACAGATGGCATCTTATTTTGGATATACCGTTGTCGT
  ATCAGATGTTAACAGTGATGGACTGGATGATGTCCTGGTTGGGGCACCTCTCTTTATGGAACGTGAATTT
  GAGAGCAACCCCAGAGAAGTAGGGCAAATCTACCTGTATTTGCAAGTGAGCTCTCTCCTCTTCAGAGACC
  CCCAGATCCTCACTGGCACCGAGACGTTTGGGAGATTCGGTAGTGCTATGGCACACTTAGGAGACCTGAA
  CCAAGATGGATACAATGACATTGCCATCGGAGTGCCTTTTGCAGGCAAGGATCAAAGAGGCAAAGTGCTC
  ATTTATAATGGGAACAAAGATGGCTTAAACACCAAGCCTTCCCAAGTTCTGCAAGGAGTGTGGGCCTCAC
  ATGCTGTCCCTTCCGGATTTGGCTTTACTTTAAGAGGAGATTCAGACATAGACAAGAATGATTACCCAGA
  TTTGATTGTGGGTGCATTTGGAACAGGAAAAGTCGCTGTTTACAGAGCAAGACCGGTTGTGACTGTAGAT
  GCCCAGCTTCTGCTGCACCCAATGATTATCAATCTTGAAAATAAAACTTGCCAGGTTCCAGACTCTATGA
  CATCTGCTGCCTGCTTTTCTTTAAGAGTATGTGCATCTGTCACAGGCCAGAGCATTGCAAACACAATAGT
  CTTGATGGCAGAGGTGCAATTAGATTCCCTGAAACAGAAAGGAGCTATTAAACGGACGCTCTTCCTTGAT
  AACCATCAGGCTCATCGCGTCTTCCCTCTTGTGATAAAAAGGCAGAAATCCCACCAGTGCCAGGATTTCA
  TCGTTTACCTTCGAGATGAAACTGAATTCCGAGATAAATTATCTCCAATCAACATTAGTTTGAATTACAG
  TTTGGACGAATCCACCTTTAAAGAAGGCCTGGAAGTGAAACCAATATTGAACTACTACAGAGAAAACATT
  GTTAGTGAACAGGCTCACATTCTGGTGGACTGTGGAGAAGACAATCTGTGTGTTCCTGACTTGAAGCTGT
  CGGCTAGACCAGATAAGCATCAGGTAATCATTGGAGATGAAAATCACCTTATGCTCATAATAAATGCAAG
  AAATGAAGGGGAAGGAGCATATGAAGCTGAACTCTTTGTAATGATACCAGAAGAGGCAGATTATGTTGGA
  ATCGAACGCAACAACAAGGGATTTCGACCACTGAGCTGTGAGTACAAGATGGAAAATGTAACCAGGATGG
  TGGTGTGTGACCTTGGGAACCCTATGGTGTCTGGAACAAATTATTCCCTGGGCCTCCGATTTGCAGTTCC
  ACGTCTTGAGAAAACAAACATGAGCATTAACTTCGATCTCCAAATCAGAAGTTCCAACAAGGACAATCCA
  GACAGCAATTTTGTGAGCCTGCAAATCAACATCACTGCTGTAGCGCAGGTGGAAATAAGAGGAGTGTCAC
  ACCCTCCGCAGATTGTTCTGCCCATTCATAACTGGGAACCAGAAGAGGAGCCCCACAAAGAGGAGGAGGT
  TGGACCATTGGTGGAACATATTTATGAGCTGCACAATATTGGACCAAGTACCATCAGTGACACCATCCTG
  GAGGTGGGCTGGCCTTTCTCTGCCCGGGATGAATTTCTTCTCTATATTTTCCATATTCAAACTCTGGGAC
  CTCTGCAGTGCCAACCAAATCCTAATATCAATCCACAGGATATAAAGCCTGCTGCCTCCCCAGAGGACAC
  CCCTGAGCTCAGCGCCTTTTTGCGAAACTCTACTATTCCTCATCTTGTCAGGAAGAGGGATGTACATGTG
  GTCGAATTCCACAGACAGAGCCCTGCAAAAATACTGAATTGTACAAATATCGAGTGTTTACAAATCTCCT
  GTGCAGTGGGACGACTCGAAGGAGGAGAAAGCGCAGTCCTGAAAGTCAGGTCACGATTATGGGCCCACAC
  CTTCCTCCAGAGAAAAAATGATCCCTATGCTCTTGCATCCCTGGTGTCCTTTGAAGTTAAGAAGATGCCT
  TATACAGATCAGCCAGCAAAACTCCCAGAAGGAAGCATAGCAATTAAGACATCAGTTATTTGGGCAACTC
  CGAATGTTTCCTTCTCAATCCCATTATGGGTAATAATACTAGCAATACTTCTTGGATTGTTGGTTCTCGC
  CATTTTAACCTTAGCTTTATGGAAGTGTGGATTCTTTGACAGAGCCAGACCTCCTCAGGAGGACATGACC
  GACAGGGAACAGCTGACAAATGACAAGACCCCTGAGGCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC218936 representing NM_003638
  Red=Cloning site Green=Tags(s)
  
  MSPGASRGPRGSQAPLIAPLCCAAAALGMLLWSPACQAFNLDVEKLTVYSGPKGSYFGYAVDFHIPDART
  ASVLVGAPKANTSQPDIVEGGAVYYCPWPAEGSAQCRQIPFDTTNNRKIRVNGTKEPIEFKSNQWFGATV
  KAHKGKVVACAPLYHWRTLKPTPEKDPVGTCYVAIQNFSAYAEFSPCRNSNADPEGQGYCQAGFSLDFYK
  NGDLIVGGPGSFYWQGQVITASVADIIANYSFKDILRKLAGEKQTEVAPASYDDSYLGYSVAAGEFTGDS
  QQELVAGIPRGAQNFGYVSIINSTDMTFIQNFTGEQMASYFGYTVVVSDVNSDGLDDVLVGAPLFMEREF
  ESNPREVGQIYLYLQVSSLLFRDPQILTGTETFGRFGSAMAHLGDLNQDGYNDIAIGVPFAGKDQRGKVL
  IYNGNKDGLNTKPSQVLQGVWASHAVPSGFGFTLRGDSDIDKNDYPDLIVGAFGTGKVAVYRARPVVTVD
  AQLLLHPMIINLENKTCQVPDSMTSAACFSLRVCASVTGQSIANTIVLMAEVQLDSLKQKGAIKRTLFLD
  NHQAHRVFPLVIKRQKSHQCQDFIVYLRDETEFRDKLSPINISLNYSLDESTFKEGLEVKPILNYYRENI
  VSEQAHILVDCGEDNLCVPDLKLSARPDKHQVIIGDENHLMLIINARNEGEGAYEAELFVMIPEEADYVG
  IERNNKGFRPLSCEYKMENVTRMVVCDLGNPMVSGTNYSLGLRFAVPRLEKTNMSINFDLQIRSSNKDNP
  DSNFVSLQINITAVAQVEIRGVSHPPQIVLPIHNWEPEEEPHKEEEVGPLVEHIYELHNIGPSTISDTIL
  EVGWPFSARDEFLLYIFHIQTLGPLQCQPNPNINPQDIKPAASPEDTPELSAFLRNSTIPHLVRKRDVHV
  VEFHRQSPAKILNCTNIECLQISCAVGRLEGGESAVLKVRSRLWAHTFLQRKNDPYALASLVSFEVKKMP
  YTDQPAKLPEGSIAIKTSVIWATPNVSFSIPLWVIILAILLGLLVLAILTLALWKCGFFDRARPPQEDMT
  DREQLTNDKTPEA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003638
• ORF Size: 3189 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003638.1, NM_003638.2, NP_003629.1, NP_003629.2
• RefSeq Size: 3261
• RefSeq ORF: 3192
• Locus ID: 8516
• Protein Families: Druggable Genome, Transmembrane
• Protein Pathways: Arrhythmogenic right ventricular cardiomyopathy (ARVC), Cell adhesion molecules (CAMs), Dilated cardiomyopathy, ECM-receptor interaction, Focal adhesion, Hypertrophic cardiomyopathy (HCM), Regulation of actin cytoskeleton
• MW: 117.3 kDa
• Gene Summary: Integrins are heterodimeric transmembrane receptor proteins that mediate numerous cellular processes including cell adhesion, cytoskeletal rearrangement, and activation of cell signaling pathways. Integrins are composed of alpha and beta subunits. This gene encodes the alpha 8 subunit of the heterodimeric integrin alpha8beta1 protein. The encoded protein is a single-pass type 1 membrane protein that contains multiple FG-GAP repeats. This repeat is predicted to fold into a beta propeller structure. This gene regulates the recruitment of mesenchymal cells into epithelial structures, mediates cell-cell interactions, and regulates neurite outgrowth of sensory and motor neurons. The integrin alpha8beta1 protein thus plays an important role in wound-healing and organogenesis. Mutations in this gene have been associated with renal hypodysplasia/aplasia-1 (RHDA1) and with several animal models of chronic kidney disease. Alternate splicing results in multiple transcript variants encoding distinct isoforms. [provided by RefSeq, Apr 2014]