SPINT1 (NM_181642) Human Tagged ORF Clone
CAT#: RC218993
SPINT1 (Myc-DDK-tagged)-Human serine peptidase inhibitor, Kunitz type 1 (SPINT1), transcript variant 1
"NM_181642" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | SPINT1 |
Synonyms | HAI; HAI1; MANSC2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC218993 representing NM_181642
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCTGCGAGGACGATGGCCCGCGCCCGCCTCGCCCCGGCCGGCATCCCTGCCGTCGCCTTGTGGC TTCTGTGCACGCTCGGCCTCCAGGGCACCCAGGCCGGGCCACCGCCCGCGCCCCCTGGGCTGCCCGCGGG AGCCGACTGCCTGAACAGCTTTACCGCCGGGGTGCCTGGCTTCGTGCTGGACACCAACGCCTCGGTCAGC AACGGAGCTACCTTCCTGGAGTCCCCCACCGTGCGCCGGGGCTGGGACTGCGTGCGCGCCTGCTGCACCA CCCAGAACTGCAACTTGGCGCTAGTGGAGCTGCAGCCCGACCGCGGGGAGGACGCCATCGCCGCCTGCTT CCTCATCAACTGCCTCTACGAGCAGAACTTCGTGTGCAAGTTCGCGCCCAGGGAGGGCTTCATCAACTAC CTCACGAGGGAAGTGTACCGCTCCTACCGCCAGCTGCGGACCCAGGGCTTTGGAGGGTCTGGGATCCCCA AGGCCTGGGCAGGCATAGACTTGAAGGTACAACCCCAGGAACCCCTGGTGCTGAAGGATGTGGAAAACAC AGATTGGCGCCTACTGCGGGGTGACACGGATGTCAGGGTAGAGAGGAAAGACCCAAACCAGGTGGAACTG TGGGGACTCAAGGAAGGCACCTACCTGTTCCAGCTGACAGTGACTAGCTCAGACCACCCAGAGGACACGG CCAACGTCACAGTCACTGTGCTGTCCACCAAGCAGACAGAAGACTACTGCCTCGCATCCAACAAGGTGGG TCGCTGCCGGGGCTCTTTCCCACGCTGGTACTATGACCCCACGGAGCAGATCTGCAAGAGTTTCGTTTAT GGAGGCTGCTTGGGCAACAAGAACAACTACCTTCGGGAAGAAGAGTGCATTCTAGCCTGTCGGGGTGTGC AAGGTGGGCCTTTGAGAGGCAGCTCTGGGGCTCAGGCGACTTTCCCCCAGGGCCCCTCCATGGAAAGGCG CCATCCAGTGTGCTCTGGCACCTGTCAGCCCACCCAGTTCCGCTGCAGCAATGGCTGCTGCATCGACAGT TTCCTGGAGTGTGACGACACCCCCAACTGCCCCGACGCCTCCGACGAGGCTGCCTGTGAAAAATACACGA GTGGCTTTGACGAGCTCCAGCGCATCCATTTCCCCAGTGACAAAGGGCACTGCGTGGACCTGCCAGACAC AGGACTCTGCAAGGAGAGCATCCCGCGCTGGTACTACAACCCCTTCAGCGAACACTGCGCCCGCTTTACC TATGGTGGTTGTTATGGCAACAAGAACAACTTTGAGGAAGAGCAGCAGTGCCTCGAGTCTTGTCGCGGCA TCTCCAAGAAGGATGTGTTTGGCCTGAGGCGGGAAATCCCCATTCCCAGCACAGGCTCTGTGGAGATGGC TGTCGCAGTGTTCCTGGTCATCTGCATTGTGGTGGTGGTAGCCATCTTGGGTTACTGCTTCTTCAAGAAC CAGAGAAAGGACTTCCACGGACACCACCACCACCCACCACCCACCCCTGCCAGCTCCACTGTCTCCACTA CCGAGGACACGGAGCACCTGGTCTATAACCACACCACGCGGCCCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218993 representing NM_181642
Red=Cloning site Green=Tags(s) MAPARTMARARLAPAGIPAVALWLLCTLGLQGTQAGPPPAPPGLPAGADCLNSFTAGVPGFVLDTNASVS NGATFLESPTVRRGWDCVRACCTTQNCNLALVELQPDRGEDAIAACFLINCLYEQNFVCKFAPREGFINY LTREVYRSYRQLRTQGFGGSGIPKAWAGIDLKVQPQEPLVLKDVENTDWRLLRGDTDVRVERKDPNQVEL WGLKEGTYLFQLTVTSSDHPEDTANVTVTVLSTKQTEDYCLASNKVGRCRGSFPRWYYDPTEQICKSFVY GGCLGNKNNYLREEECILACRGVQGGPLRGSSGAQATFPQGPSMERRHPVCSGTCQPTQFRCSNGCCIDS FLECDDTPNCPDASDEAACEKYTSGFDELQRIHFPSDKGHCVDLPDTGLCKESIPRWYYNPFSEHCARFT YGGCYGNKNNFEEEQQCLESCRGISKKDVFGLRREIPIPSTGSVEMAVAVFLVICIVVVVAILGYCFFKN QRKDFHGHHHHPPPTPASSTVSTTEDTEHLVYNHTTRPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_181642 |
ORF Size | 1587 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_181642.1, NM_181642.2, NP_857593.1 |
RefSeq Size | 2484 bp |
RefSeq ORF | 1590 bp |
Locus ID | 6692 |
Cytogenetics | 15q15.1 |
Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
MW | 54.8 kDa |
Gene Summary | 'The protein encoded by this gene is a member of the Kunitz family of serine protease inhibitors. The protein is a potent inhibitor specific for HGF activator and is thought to be involved in the regulation of the proteolytic activation of HGF in injured tissues. Alternative splicing results in multiple variants encoding different isoforms. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC310294 | SPINT1 (untagged)-Human serine peptidase inhibitor, Kunitz type 1 (SPINT1), transcript variant 1 |
USD 900.00 |
|
RG218993 | SPINT1 (GFP-tagged) - Human serine peptidase inhibitor, Kunitz type 1 (SPINT1), transcript variant 1 |
USD 530.00 |
|
RC218993L1 | Lenti-ORF clone of SPINT1 (Myc-DDK-tagged)-Human serine peptidase inhibitor, Kunitz type 1 (SPINT1), transcript variant 1 |
USD 840.00 |
|
RC218993L2 | Lenti-ORF clone of SPINT1 (mGFP-tagged)-Human serine peptidase inhibitor, Kunitz type 1 (SPINT1), transcript variant 1 |
USD 680.00 |
|
RC218993L3 | Lenti-ORF clone of SPINT1 (Myc-DDK-tagged)-Human serine peptidase inhibitor, Kunitz type 1 (SPINT1), transcript variant 1 |
USD 680.00 |
|
RC218993L4 | Lenti-ORF clone of SPINT1 (mGFP-tagged)-Human serine peptidase inhibitor, Kunitz type 1 (SPINT1), transcript variant 1 |
USD 680.00 |
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