TCF19 (NM_007109) Human Tagged ORF Clone

CAT#: RC219029

TCF19 (Myc-DDK-tagged)-Human transcription factor 19 (TCF19), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TCF19
• Synonyms: SC1; TCF-19
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219029 representing NM_007109
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCTGCCCTGCTTCCAACTGCTGCGCATAGGGGGCGGCAGGGGCGGTGATCTCTACACCTTCCACCCCC
  CCGCCGGGGCTGGCTGCACCTATCGCTTGGGCCACAGGGCCGACCTGTGTGATGTGGCCCTGCGGCCCCA
  GCAGGAGCCTGGCCTCATCTCTGGGATCCACGCCGAACTGCATGCCGAGCCCCGGGGTGATGACTGGAGG
  GTCAGCCTGGAAGACCACAGCAGCCAAGGTACTTTGGTCAATAATGTCCGACTCCCAAGAGGTCACAGGC
  TGGAATTGAGTGATGGAGACCTCCTGACCTTTGGCCCTGAAGGGCCCCCAGGAACCAGCCCCTCGGAGTT
  CTACTTCATGTTCCAACAAGTACGAGTCAAGCCTCAGGACTTTGCTGCCATTACCATCCCACGGTCTAGG
  GGAGAAGCCCGGGTTGGGGCTGGTTTCCGGCCTATGCTGCCCTCCCAGGGGGCTCCACAGCGGCCTCTCA
  GCACCTTCTCCCCTGCCCCCAAGGCCACACTGATCCTAAACTCCATAGGCAGCCTCAGCAAGCTCCGGCC
  CCAGCCCCTCACCTTCTCCCCTAGTTGGGGTGGACCAAAGAGCCTGCCTGTTCCCGCCCCACCTGGGGAA
  ATGGGGACCACGCCTTCTGCTCCACCACAACGCAATCGGAGGAAATCTGTTCACCGAGTGTTGGCGGAAC
  TGGATGATGAGAGTGAGCCTCCTGAGAACCCGCCACCGGTCCTTATGGAGCCCAGGAAGAAACTCCGTGT
  AGACAAAGCCCCACTGACTCCCACTGGAAATCGACGTGGCCGTCCTCGGAAGTACCCAGTGAGCGCTCCC
  ATGGCTCCCCCTGCAGTTGGGGGCGGGGAGCCCTGTGCAGCTCCTTGTTGCTGCCTGCCCCAGGAAGAGA
  CAGTGGCCTGGGTTCAGTGTGATGGCTGTGACGTCTGGTTCCATGTGGCCTGTGTTGGCTGCAGCATCCA
  GGCTGCCAGGGAGGCCGACTTCCGATGCCCAGGGTGCCGGGCTGGCATTCAGACC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219029 representing NM_007109
  Red=Cloning site Green=Tags(s)
  
  MLPCFQLLRIGGGRGGDLYTFHPPAGAGCTYRLGHRADLCDVALRPQQEPGLISGIHAELHAEPRGDDWR
  VSLEDHSSQGTLVNNVRLPRGHRLELSDGDLLTFGPEGPPGTSPSEFYFMFQQVRVKPQDFAAITIPRSR
  GEARVGAGFRPMLPSQGAPQRPLSTFSPAPKATLILNSIGSLSKLRPQPLTFSPSWGGPKSLPVPAPPGE
  MGTTPSAPPQRNRRKSVHRVLAELDDESEPPENPPPVLMEPRKKLRVDKAPLTPTGNRRGRPRKYPVSAP
  MAPPAVGGGEPCAAPCCCLPQEETVAWVQCDGCDVWFHVACVGCSIQAAREADFRCPGCRAGIQT
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007109
• ORF Size: 1035 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007109.2, NP_009040.2
• RefSeq Size: 3306 bp
• RefSeq ORF: 1038 bp
• Locus ID: 6941
• Cytogenetics: 6p21.33
• Domains: FHA
• Protein Families: Druggable Genome, Transcription Factors
• MW: 37.2 kDa
• Gene Summary: 'This gene encodes a protein that contains a PHD-type zinc finger domain and likely functions as a transcription factor. The encoded protein plays a role proliferation and apoptosis of pancreatic beta cells. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Jan 2016]'