GLUR3 (GRIA3) (NM_007325) Human Tagged ORF Clone

CAT#: RC219063

GRIA3 (Myc-DDK-tagged)-Human glutamate receptor, ionotrophic, AMPA 3 (GRIA3), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY416052) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219063 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GRIA3
• Synonyms: GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC; MRX94
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219063 representing NM_007325
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCAGGCAGAAGAAAATGGGGCAAAGCGTGCTCCGGGCGGTCTTCTTTTTAGTCCTGGGGCTTTTGG
  GTCATTCTCACGGAGGATTCCCCAACACCATCAGCATAGGTGGACTTTTCATGAGAAACACAGTGCAGGA
  GCACAGCGCTTTCCGCTTTGCCGTGCAGTTATACAACACCAACCAGAACACCACCGAGAAGCCCTTCCAT
  TTGAATTACCACGTAGATCACTTGGATTCCTCCAATAGTTTTTCCGTGACAAATGCTTTCTGCTCCCAGT
  TCTCGAGAGGGGTGTATGCCATCTTTGGATTCTATGACCAGATGTCAATGAACACCCTGACCTCCTTCTG
  TGGGGCCCTGCACACATCCTTTGTTACGCCTAGCTTCCCCACTGACGCAGATGTGCAGTTTGTCATCCAG
  ATGCGCCCAGCCTTGAAGGGCGCTATTCTGAGTCTTCTGGGTCATTACAAGTGGGAGAAGTTTGTGTACC
  TCTATGACACAGAACGAGGATTTTCCATCCTCCAAGCGATTATGGAAGCAGCAGTGCAAAACAACTGGCA
  AGTAACAGCAAGGTCTGTGGGAAACATAAAGGACGTCCAAGAATTCAGGCGCATCATTGAAGAAATGGAC
  AGGAGGCAGGAAAAGCGATACTTGATTGACTGCGAAGTCGAAAGGATTAACACAATTTTGGAACAGGTTG
  TGATCCTAGGGAAACACTCAAGAGGTTATCACTACATGCTCGCTAACCTGGGTTTTACTGATATTTTACT
  GGAAAGAGTCATGCATGGGGGAGCCAACATTACAGGTTTCCAGATTGTCAACAATGAAAACCCTATGGTT
  CAGCAGTTCATACAGCGCTGGGTGAGGCTGGATGAAAGGGAATTCCCTGAAGCCAAGAATGCACCACTAA
  AGTATACATCTGCATTGACACACGACGCAATACTGGTCATAGCAGAAGCTTTCCGCTACCTGAGGAGGCA
  GCGAGTAGATGTGTCCCGGAGAGGAAGTGCTGGAGACTGCTTAGCAAATCCTGCTGTGCCCTGGAGTCAA
  GGAATTGATATTGAGAGAGCTCTGAAAATGGTGCAAGTACAAGGAATGACTGGAAATATTCAATTTGACA
  CTTATGGACGTAGGACAAATTATACCATCGATGTGTATGAAATGAAAGTCAGTGGCTCTCGAAAAGCTGG
  CTACTGGAATGAGTATGAAAGGTTTGTGCCTTTCTCAGATCAGCAAATCAGCAATGACAGTGCATCCTCA
  GAGAATCGGACCATAGTAGTGACTACCATTCTGGAATCACCATATGTAATGTACAAGAAGAACCATGAGC
  AACTGGAAGGAAATGAACGATATGAAGGCTATTGTGTAGACCTAGCCTATGAAATAGCCAAACATGTAAG
  GATCAAATACAAATTGTCCATCGTTGGTGACGGGAAATATGGTGCAAGGGATCCAGAGACTAAAATATGG
  AACGGCATGGTTGGGGAACTTGTCTATGGGAGAGCTGATATAGCTGTTGCTCCACTCACTATAACATTGG
  TCCGTGAAGAAGTCATAGATTTTTCAAAGCCATTCATGAGCCTGGGCATCTCCATCATGATAAAGAAGCC
  TCAGAAATCAAAACCAGGCGTATTCTCATTTCTGGATCCCCTGGCTTATGAAATCTGGATGTGCATTGTC
  TTTGCTTACATTGGAGTCAGCGTAGTTCTTTTCCTAGTCAGCAGGTTCAGTCCTTATGAATGGCACTTGG
  AAGACAACAATGAAGAACCTCGTGACCCACAAAGTCCTCCTGATCCTCCAAATGAATTTGGAATATTTAA
  CAGTCTTTGGTTTTCCTTGGGTGCCTTTATGCAGCAAGGATGTGATATTTCTCCAAGATCACTCTCCGGG
  CGCATTGTTGGAGGGGTTTGGTGGTTCTTCACCCTGATCATAATTTCTTCCTATACTGCCAATCTCGCTG
  CTTTCCTGACTGTGGAGAGGATGGTTTCTCCCATAGAGAGTGCTGAAGACTTAGCTAAACAGACTGAAAT
  TGCATATGGGACCCTGGACTCCGGTTCAACAAAAGAATTTTTCAGAAGATCCAAAATTGCTGTGTACGAG
  AAAATGTGGTCTTACATGAAATCAGCGGAGCCATCTGTGTTTACCAAAACAACAGCAGACGGAGTGGCCC
  GAGTGCGAAAGTCCAAGGGAAAGTTCGCCTTCCTGCTGGAGTCAACCATGAATGAGTACATTGAGCAGAG
  AAAACCATGTGATACGATGAAAGTTGGTGGAAATCTGGATTCCAAAGGCTATGGTGTGGCAACCCCTAAA
  GGCTCAGCATTAAGAACGCCTGTAAACCTTGCAGTATTGAAACTCAGTGAACAAGGCATCTTAGACAAGC
  TGAAAAACAAATGGTGGTACGATAAGGGGGAATGTGGAGCCAAGGACTCCGGGAGTAAGGACAAGACCAG
  CGCTCTGAGCCTGAGCAATGTGGCAGGCGTTTTCTATATACTTGTCGGAGGTCTGGGGCTGGCCATGATG
  GTGGCTTTGATAGAATTCTGTTACAAATCACGGGCAGAGTCCAAACGCATGAAACTCACAAAGAACACCC
  AAAACTTTAAGCCTGCTCCTGCCACCAACACTCAGAATTATGCTACATACAGAGAAGGCTACAACGTGTA
  TGGAACAGAGAGTGTTAAGATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219063 representing NM_007325
  Red=Cloning site Green=Tags(s)
  
  MARQKKMGQSVLRAVFFLVLGLLGHSHGGFPNTISIGGLFMRNTVQEHSAFRFAVQLYNTNQNTTEKPFH
  LNYHVDHLDSSNSFSVTNAFCSQFSRGVYAIFGFYDQMSMNTLTSFCGALHTSFVTPSFPTDADVQFVIQ
  MRPALKGAILSLLGHYKWEKFVYLYDTERGFSILQAIMEAAVQNNWQVTARSVGNIKDVQEFRRIIEEMD
  RRQEKRYLIDCEVERINTILEQVVILGKHSRGYHYMLANLGFTDILLERVMHGGANITGFQIVNNENPMV
  QQFIQRWVRLDEREFPEAKNAPLKYTSALTHDAILVIAEAFRYLRRQRVDVSRRGSAGDCLANPAVPWSQ
  GIDIERALKMVQVQGMTGNIQFDTYGRRTNYTIDVYEMKVSGSRKAGYWNEYERFVPFSDQQISNDSASS
  ENRTIVVTTILESPYVMYKKNHEQLEGNERYEGYCVDLAYEIAKHVRIKYKLSIVGDGKYGARDPETKIW
  NGMVGELVYGRADIAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIV
  FAYIGVSVVLFLVSRFSPYEWHLEDNNEEPRDPQSPPDPPNEFGIFNSLWFSLGAFMQQGCDISPRSLSG
  RIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDSGSTKEFFRRSKIAVYE
  KMWSYMKSAEPSVFTKTTADGVARVRKSKGKFAFLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGVATPK
  GSALRTPVNLAVLKLSEQGILDKLKNKWWYDKGECGAKDSGSKDKTSALSLSNVAGVFYILVGGLGLAMM
  VALIEFCYKSRAESKRMKLTKNTQNFKPAPATNTQNYATYREGYNVYGTESVKI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007325
• ORF Size: 2682 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007325.1, NM_007325.2, NM_007325.3, NM_007325.4, NP_015564.2
• RefSeq Size: 3148 bp
• RefSeq ORF: 2685 bp
• Locus ID: 2892
• Cytogenetics: Xq25
• Protein Families: Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane
• Protein Pathways: Long-term depression, Neuroactive ligand-receptor interaction
• MW: 101.23 kDa
• Gene Summary: 'Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing at this locus results in different isoforms, which may vary in their signal transduction properties. [provided by RefSeq, Jul 2008]'