GLUR3 (GRIA3) (NM_007325) Human Tagged ORF Clone
CAT#: RC219063
GRIA3 (Myc-DDK-tagged)-Human glutamate receptor, ionotrophic, AMPA 3 (GRIA3), transcript variant 1
"NM_007325" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | GRIA3 |
Synonyms | GluA3; GLUR-C; GLUR-K3; GLUR3; GLURC; MRX94 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC219063 representing NM_007325
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCAGGCAGAAGAAAATGGGGCAAAGCGTGCTCCGGGCGGTCTTCTTTTTAGTCCTGGGGCTTTTGG GTCATTCTCACGGAGGATTCCCCAACACCATCAGCATAGGTGGACTTTTCATGAGAAACACAGTGCAGGA GCACAGCGCTTTCCGCTTTGCCGTGCAGTTATACAACACCAACCAGAACACCACCGAGAAGCCCTTCCAT TTGAATTACCACGTAGATCACTTGGATTCCTCCAATAGTTTTTCCGTGACAAATGCTTTCTGCTCCCAGT TCTCGAGAGGGGTGTATGCCATCTTTGGATTCTATGACCAGATGTCAATGAACACCCTGACCTCCTTCTG TGGGGCCCTGCACACATCCTTTGTTACGCCTAGCTTCCCCACTGACGCAGATGTGCAGTTTGTCATCCAG ATGCGCCCAGCCTTGAAGGGCGCTATTCTGAGTCTTCTGGGTCATTACAAGTGGGAGAAGTTTGTGTACC TCTATGACACAGAACGAGGATTTTCCATCCTCCAAGCGATTATGGAAGCAGCAGTGCAAAACAACTGGCA AGTAACAGCAAGGTCTGTGGGAAACATAAAGGACGTCCAAGAATTCAGGCGCATCATTGAAGAAATGGAC AGGAGGCAGGAAAAGCGATACTTGATTGACTGCGAAGTCGAAAGGATTAACACAATTTTGGAACAGGTTG TGATCCTAGGGAAACACTCAAGAGGTTATCACTACATGCTCGCTAACCTGGGTTTTACTGATATTTTACT GGAAAGAGTCATGCATGGGGGAGCCAACATTACAGGTTTCCAGATTGTCAACAATGAAAACCCTATGGTT CAGCAGTTCATACAGCGCTGGGTGAGGCTGGATGAAAGGGAATTCCCTGAAGCCAAGAATGCACCACTAA AGTATACATCTGCATTGACACACGACGCAATACTGGTCATAGCAGAAGCTTTCCGCTACCTGAGGAGGCA GCGAGTAGATGTGTCCCGGAGAGGAAGTGCTGGAGACTGCTTAGCAAATCCTGCTGTGCCCTGGAGTCAA GGAATTGATATTGAGAGAGCTCTGAAAATGGTGCAAGTACAAGGAATGACTGGAAATATTCAATTTGACA CTTATGGACGTAGGACAAATTATACCATCGATGTGTATGAAATGAAAGTCAGTGGCTCTCGAAAAGCTGG CTACTGGAATGAGTATGAAAGGTTTGTGCCTTTCTCAGATCAGCAAATCAGCAATGACAGTGCATCCTCA GAGAATCGGACCATAGTAGTGACTACCATTCTGGAATCACCATATGTAATGTACAAGAAGAACCATGAGC AACTGGAAGGAAATGAACGATATGAAGGCTATTGTGTAGACCTAGCCTATGAAATAGCCAAACATGTAAG GATCAAATACAAATTGTCCATCGTTGGTGACGGGAAATATGGTGCAAGGGATCCAGAGACTAAAATATGG AACGGCATGGTTGGGGAACTTGTCTATGGGAGAGCTGATATAGCTGTTGCTCCACTCACTATAACATTGG TCCGTGAAGAAGTCATAGATTTTTCAAAGCCATTCATGAGCCTGGGCATCTCCATCATGATAAAGAAGCC TCAGAAATCAAAACCAGGCGTATTCTCATTTCTGGATCCCCTGGCTTATGAAATCTGGATGTGCATTGTC TTTGCTTACATTGGAGTCAGCGTAGTTCTTTTCCTAGTCAGCAGGTTCAGTCCTTATGAATGGCACTTGG AAGACAACAATGAAGAACCTCGTGACCCACAAAGTCCTCCTGATCCTCCAAATGAATTTGGAATATTTAA CAGTCTTTGGTTTTCCTTGGGTGCCTTTATGCAGCAAGGATGTGATATTTCTCCAAGATCACTCTCCGGG CGCATTGTTGGAGGGGTTTGGTGGTTCTTCACCCTGATCATAATTTCTTCCTATACTGCCAATCTCGCTG CTTTCCTGACTGTGGAGAGGATGGTTTCTCCCATAGAGAGTGCTGAAGACTTAGCTAAACAGACTGAAAT TGCATATGGGACCCTGGACTCCGGTTCAACAAAAGAATTTTTCAGAAGATCCAAAATTGCTGTGTACGAG AAAATGTGGTCTTACATGAAATCAGCGGAGCCATCTGTGTTTACCAAAACAACAGCAGACGGAGTGGCCC GAGTGCGAAAGTCCAAGGGAAAGTTCGCCTTCCTGCTGGAGTCAACCATGAATGAGTACATTGAGCAGAG AAAACCATGTGATACGATGAAAGTTGGTGGAAATCTGGATTCCAAAGGCTATGGTGTGGCAACCCCTAAA GGCTCAGCATTAAGAACGCCTGTAAACCTTGCAGTATTGAAACTCAGTGAACAAGGCATCTTAGACAAGC TGAAAAACAAATGGTGGTACGATAAGGGGGAATGTGGAGCCAAGGACTCCGGGAGTAAGGACAAGACCAG CGCTCTGAGCCTGAGCAATGTGGCAGGCGTTTTCTATATACTTGTCGGAGGTCTGGGGCTGGCCATGATG GTGGCTTTGATAGAATTCTGTTACAAATCACGGGCAGAGTCCAAACGCATGAAACTCACAAAGAACACCC AAAACTTTAAGCCTGCTCCTGCCACCAACACTCAGAATTATGCTACATACAGAGAAGGCTACAACGTGTA TGGAACAGAGAGTGTTAAGATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219063 representing NM_007325
Red=Cloning site Green=Tags(s) MARQKKMGQSVLRAVFFLVLGLLGHSHGGFPNTISIGGLFMRNTVQEHSAFRFAVQLYNTNQNTTEKPFH LNYHVDHLDSSNSFSVTNAFCSQFSRGVYAIFGFYDQMSMNTLTSFCGALHTSFVTPSFPTDADVQFVIQ MRPALKGAILSLLGHYKWEKFVYLYDTERGFSILQAIMEAAVQNNWQVTARSVGNIKDVQEFRRIIEEMD RRQEKRYLIDCEVERINTILEQVVILGKHSRGYHYMLANLGFTDILLERVMHGGANITGFQIVNNENPMV QQFIQRWVRLDEREFPEAKNAPLKYTSALTHDAILVIAEAFRYLRRQRVDVSRRGSAGDCLANPAVPWSQ GIDIERALKMVQVQGMTGNIQFDTYGRRTNYTIDVYEMKVSGSRKAGYWNEYERFVPFSDQQISNDSASS ENRTIVVTTILESPYVMYKKNHEQLEGNERYEGYCVDLAYEIAKHVRIKYKLSIVGDGKYGARDPETKIW NGMVGELVYGRADIAVAPLTITLVREEVIDFSKPFMSLGISIMIKKPQKSKPGVFSFLDPLAYEIWMCIV FAYIGVSVVLFLVSRFSPYEWHLEDNNEEPRDPQSPPDPPNEFGIFNSLWFSLGAFMQQGCDISPRSLSG RIVGGVWWFFTLIIISSYTANLAAFLTVERMVSPIESAEDLAKQTEIAYGTLDSGSTKEFFRRSKIAVYE KMWSYMKSAEPSVFTKTTADGVARVRKSKGKFAFLLESTMNEYIEQRKPCDTMKVGGNLDSKGYGVATPK GSALRTPVNLAVLKLSEQGILDKLKNKWWYDKGECGAKDSGSKDKTSALSLSNVAGVFYILVGGLGLAMM VALIEFCYKSRAESKRMKLTKNTQNFKPAPATNTQNYATYREGYNVYGTESVKI TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_007325 |
ORF Size | 2682 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_007325.1, NM_007325.2, NM_007325.3, NM_007325.4, NP_015564.2 |
RefSeq Size | 3148 bp |
RefSeq ORF | 2685 bp |
Locus ID | 2892 |
Cytogenetics | Xq25 |
Protein Families | Druggable Genome, Ion Channels: Glutamate Receptors, Transmembrane |
Protein Pathways | Long-term depression, Neuroactive ligand-receptor interaction |
MW | 101.23 kDa |
Gene Summary | 'Glutamate receptors are the predominant excitatory neurotransmitter receptors in the mammalian brain and are activated in a variety of normal neurophysiologic processes. These receptors are heteromeric protein complexes composed of multiple subunits, arranged to form ligand-gated ion channels. The classification of glutamate receptors is based on their activation by different pharmacologic agonists. The subunit encoded by this gene belongs to a family of AMPA (alpha-amino-3-hydroxy-5-methyl-4-isoxazole propionate)-sensitive glutamate receptors, and is subject to RNA editing (AGA->GGA; R->G). Alternative splicing at this locus results in different isoforms, which may vary in their signal transduction properties. [provided by RefSeq, Jul 2008]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC127844 | GRIA3 (untagged)-Human glutamate receptor, ionotrophic, AMPA 3 (GRIA3), transcript variant 1 |
USD 1,810.00 |
|
RG219063 | GRIA3 (GFP-tagged) - Human glutamate receptor, ionotrophic, AMPA 3 (GRIA3), transcript variant 1 |
USD 830.00 |
|
RC219063L1 | Lenti ORF clone of Human glutamate receptor, ionotrophic, AMPA 3 (GRIA3), transcript variant 1, Myc-DDK-tagged |
USD 1,176.00 |
|
RC219063L2 | Lenti ORF clone of Human glutamate receptor, ionotrophic, AMPA 3 (GRIA3), transcript variant 1, mGFP tagged |
USD 950.00 |
|
RC219063L3 | Lenti ORF clone of Human glutamate receptor, ionotrophic, AMPA 3 (GRIA3), transcript variant 1, Myc-DDK-tagged |
USD 950.00 |
|
RC219063L4 | Lenti ORF clone of Human glutamate receptor, ionotrophic, AMPA 3 (GRIA3), transcript variant 1, mGFP tagged |
USD 950.00 |
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