SNX18 (NM_052870) Human Tagged ORF Clone

CAT#: RC219150

SNX18 (Myc-DDK-tagged)-Human sorting nexin 18 (SNX18), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY403272) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219150 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SNX18
• Synonyms: SH3PX2; SH3PXD3B; SNAG1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219150 representing NM_052870
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGCTGCGCGCCCGGGCGCTGTACGACTTCAGGTCGGAGAACCCAGGAGAGATCTCGCTGCGAGAGC
  ACGAGGTGCTGAGCCTGTGCAGCGAGCAGGACATCGAGGGCTGGCTCGAGGGGGTCAACAGCCGCGGCGA
  CCGCGGCCTCTTCCCGGCCTCCTATGTGCAGGTGATCCGCGCCCCCGAGCCTGGCCCGGCGGGAGACGGC
  GGCCCGGGCGCCCCGGCCCGCTACGCCAATGTGCCCCCCGGGGGCTTCGAGCCCCTGCCCGTCGCGCCCC
  CCGCCTCCTTCAAGCCGCCGCCTGACGCCTTCCAGGCGCTGCTGCAGCCACAGCAGGCGCCGCCTCCGAG
  CACCTTCCAGCCGCCCGGCGCGGGCTTCCCGTACGGCGGGGGCGCCCTGCAGCCGTCGCCTCAGCAGCTC
  TACGGCGGCTACCAGGCCAGCCAAGGCAGCGATGATGACTGGGACGACGAGTGGGACGACAGCTCCACGG
  TGGCGGACGAGCCGGGCGCTCTGGGCAGCGGAGCATACCCGGACCTCGACGGCTCGTCTTCGGCGGGTGT
  GGGCGCAGCCGGCCGCTACCGCCTGTCCACGCGCTCCGACCTGTCCCTGGGCTCCCGCGGCGGCTCGGTC
  CCCCCGCAGCACCACCCGTCGGGGCCCAAGAGCTCGGCCACCGTGAGCCGCAACCTCAATCGCTTCTCCA
  CCTTCGTCAAGTCCGGCGGGGAGGCCTTCGTGCTGGGGGAGGCGTCAGGCTTCGTGAAGGACGGGGACAA
  GCTGTGCGTGGTGCTGGGGCCCTATGGCCCCGAGTGGCAGGAGAACCCCTACCCGTTCCAGTGCACCATC
  GACGACCCCACCAAGCAGACCAAGTTCAAGGGCATGAAGAGCTACATCTCCTACAAGCTGGTGCCCACGC
  ACACGCAGGTGCCGGTGCATCGGCGCTACAAGCACTTCGACTGGCTGTACGCGCGCCTGGCGGAGAAGTT
  CCCGGTCATCTCCGTGCCCCACCTGCCCGAGAAGCAGGCCACCGGCCGCTTCGAGGAGGACTTCATCTCT
  AAGCGCAGGAAGGGCCTGATCTGGTGGATGAACCACATGGCCAGCCACCCAGTGCTGGCGCAGTGCGACG
  TCTTCCAGCACTTCCTGACGTGCCCCAGCAGCACCGACGAGAAAGCCTGGAAGCAGGGCAAGAGGAAGGC
  CGAGAAGGACGAGATGGTGGGCGCCAACTTCTTCCTGACCCTTAGCACGCCCCCCGCCGCTGCCCTTGAC
  CTGCAGGAGGTGGAGAGCAAGATCGACGGCTTCAAGTGCTTCACCAAGAAGATGGACGACAGCGCGCTGC
  AGCTCAACCACACGGCCAACGAGTTCGCGCGCAAGCAGGTGACCGGCTTCAAAAAGGAGTATCAGAAGGT
  GGGCCAGTCCTTCCGCGGCCTCAGCCAGGCCTTTGAGCTGGACCAGCAGGCCTTCTCGGTGGGCCTGAAC
  CAGGCTATCGCCTTCACCGGAGATGCCTATGACGCCATTGGCGAGCTCTTCGCGGAGCAGCCCAGGCAGG
  ACCTGGATCCCGTCATGGACCTATTAGCGCTGTATCAGGGGCATCTGGCTAACTTCCCGGACATCATCCA
  CGTTCAGAAAGGTAAAGCCTGGCCCTTAGAGCAGGTGATATGGAGTGTATTGTGCAGGCTGAAAGGGGCG
  ACTTTGACAGCAGTACCACTGTGGGTTTCAGAATCATATTCTACAGGTGAGGAAGCGAGCAGAGACGTGG
  ACGCCTGGGTCTTTTCCCTAGAGTGTACGTTGGATTGCTCGACAGGCAGCTTCCTCCTCGAGTATCTTGC
  ATTAGGGAATGAGTACTCTTTCTCGAAGGTTCAAAGAGTACCTTTGATGACAGTGCTATCATTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219150 representing NM_052870
  Red=Cloning site Green=Tags(s)
  
  MALRARALYDFRSENPGEISLREHEVLSLCSEQDIEGWLEGVNSRGDRGLFPASYVQVIRAPEPGPAGDG
  GPGAPARYANVPPGGFEPLPVAPPASFKPPPDAFQALLQPQQAPPPSTFQPPGAGFPYGGGALQPSPQQL
  YGGYQASQGSDDDWDDEWDDSSTVADEPGALGSGAYPDLDGSSSAGVGAAGRYRLSTRSDLSLGSRGGSV
  PPQHHPSGPKSSATVSRNLNRFSTFVKSGGEAFVLGEASGFVKDGDKLCVVLGPYGPEWQENPYPFQCTI
  DDPTKQTKFKGMKSYISYKLVPTHTQVPVHRRYKHFDWLYARLAEKFPVISVPHLPEKQATGRFEEDFIS
  KRRKGLIWWMNHMASHPVLAQCDVFQHFLTCPSSTDEKAWKQGKRKAEKDEMVGANFFLTLSTPPAAALD
  LQEVESKIDGFKCFTKKMDDSALQLNHTANEFARKQVTGFKKEYQKVGQSFRGLSQAFELDQQAFSVGLN
  QAIAFTGDAYDAIGELFAEQPRQDLDPVMDLLALYQGHLANFPDIIHVQKGKAWPLEQVIWSVLCRLKGA
  TLTAVPLWVSESYSTGEEASRDVDAWVFSLECTLDCSTGSFLLEYLALGNEYSFSKVQRVPLMTVLSF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052870
• ORF Size: 1884 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_052870.1, NM_052870.2, NP_443102.1, NP_443102.2
• RefSeq Size: 1887
• RefSeq ORF: 1887
• Locus ID: 112574
• Domains: SH3, PX
• MW: 68.7 kDa
• Gene Summary: This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein does not contain a coiled coil region, like some family members, but contains a SH3 domain. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]