ZIC4 (NM_032153) Human Tagged ORF Clone

CAT#: RC219301

ZIC4 (Myc-DDK-tagged)-Human Zic family member 4 (ZIC4), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY410308) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219301 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZIC4
• Synonyms: FLJ42609; FLJ45833
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219301 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGATACAAGACATCCTTGGTGATGAGGAAACGATTACGGCTTTACCGAAACACTCTTAAAGAGTCAA
  GTAGCAGCTCTGGACACCATGGCCCCCAGCTCACCGCCGCCTCCAGCCCCTCGGTGTTCCCGGGCCTCCA
  CGAGGAGCCTCCCCAGGCCTCCCCCAGCCGTCCTTTGAATGGACTCCTGCGTCTGGGGCTCCCTGGAGAC
  ATGTACGCGCGGCCGGAGCCCTTCCCGCCAGGGCCTGCGGCCCGCAGCGACGCCCTGGCAGCTGCCGCAG
  CCCTGCATGGCTACGGGGGCATGAACCTGACGGTGAACCTCGCTGCGCCCCACGGTCCTGGCGCTTTCTT
  CCGCTACATGCGCCAGCCCATCAAACAGGAGCTCATCTGCAAGTGGCTGGCGGCCGACGGCACCGCGACC
  CCGAGCCTCTGCTCCAAAACTTTCAGCACCATGCACGAGCTGGTCACGCACGTCACCGTGGAGCACGTCG
  GCGGCCCGGAACAGGCCAACCACATTTGCTTCTGGGAGGAGTGTCCGCGCCAGGGAAAGCCCTTCAAAGC
  CAAATACAAACTTGTAAATCACATCCGCGTGCACACGGGCGAGAAGCCCTTCCCTTGTCCTTTCCCGGGG
  TGTGGGAAGGTCTTTGCTAGATCAGAAAATCTCAAAATACACAAACGAACTCACACAGGCGAGAAGCCCT
  TCAGATGCGAGTTCGAGGGCTGCGAGCGGCGCTTCGCCAACAGCAGCGACCGTAAGAAGCATTCGCACGT
  GCACACTAGCGACAAGCCATACACGTGCAAGGTGCGGGGCTGCGACAAGTGCTACACGCACCCCAGCTCG
  CTGCGTAAGCACATGAAGGTGCACGGGCGCTCGCCGCCGCCCAGCTCTGGCTACGATTCGGCTACACCGT
  CTGCCCTCGTGTCGCCCTCGTCGGACTGCGGCCACAAGTCCCAGGTGGCCTCCTCGGCGGCGGTGGCGGC
  GCGTACCGCCGACTTGAGCGAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219301 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MRYKTSLVMRKRLRLYRNTLKESSSSSGHHGPQLTAASSPSVFPGLHEEPPQASPSRPLNGLLRLGLPGD
  MYARPEPFPPGPAARSDALAAAAALHGYGGMNLTVNLAAPHGPGAFFRYMRQPIKQELICKWLAADGTAT
  PSLCSKTFSTMHELVTHVTVEHVGGPEQANHICFWEECPRQGKPFKAKYKLVNHIRVHTGEKPFPCPFPG
  CGKVFARSENLKIHKRTHTGEKPFRCEFEGCERRFANSSDRKKHSHVHTSDKPYTCKVRGCDKCYTHPSS
  LRKHMKVHGRSPPPSSGYDSATPSALVSPSSDCGHKSQVASSAAVAARTADLSE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032153
• ORF Size: 1002 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032153.2, NM_032153.3, NM_032153.4, NM_032153.5, NP_115529.2
• RefSeq Size: 4278 bp
• RefSeq ORF: 1005 bp
• Locus ID: 84107
• Domains: zf-C2H2
• MW: 36.6 kDa
• Gene Summary: This gene encodes a member of the ZIC family of C2H2-type zinc finger proteins. Members of this family are important during development, and have been associated with X-linked visceral heterotaxy and holoprosencephaly type 5. This gene is closely linked to the gene encoding zinc finger protein of the cerebellum 1, a related family member on chromosome 3. Heterozygous deletion of these linked genes is involved in Dandy-Walker malformation, which is a congenital cerebellar malformation. Multiple transcript variants have been identified for this gene. [provided by RefSeq, Dec 2009]