RPP40 (NM_006638) Human Tagged ORF Clone

CAT#: RC219319

RPP40 (Myc-DDK-tagged)-Human ribonuclease P/MRP 40kDa subunit (RPP40)

Product Images

Western blot validation of overexpression lysate (Cat# LY416512) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219319 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified RPP40 protein (Cat# TP319319). The protein was produced from HEK293T cells transfected with RPP40 cDNA clone (Cat# RC219319) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RPP40
• Synonyms: bA428J1.3; RNASEP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219319 representing NM_006638
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCACGCTGCGCCGGCTTCGGGAGGCGCCGCGGCACTTACTGGTTTGCGAGAAATCCAACTTCGGCA
  ACCACAAGTCGCGCCACCGGCATCTTGTGCAGACGCACTACTATAACTACAGGGTTTCATTTCTCATTCC
  TGAATGTGGGATACTATCGGAAGAACTGAAAAACCTGGTCATGAACACTGGACCCTATTACTTTGTGAAG
  AATTTACCTCTTCATGAATTAATTACACCTGAATTCATCAGTACCTTTATAAAGAAAGGTTCTTGCTATG
  CACTAACATACAATACACATATTGATGAAGATAATACTGTTGCCCTGCTACCAAATGGGAAATTAATTTT
  GTCACTGGATAAAGACACTTATGAAGAAACTGGACTTCAGGGTCATCCATCTCAGTTTTCTGGCAGAAAA
  ATTATGAAATTTATTGTTTCCATTGATTTGATGGAATTATCCTTAAACTTGGATTCTAAGAAGTATGAAA
  GAATATCTTGGTCTTTCAAAGAAAAGAAGCCATTGAAATTTGATTTTCTTTTGGCTTGGCATAAAACAGG
  TTCAGAAGAATCGACAATGATGTCATATTTTTCCAAGTACCAAATTCAGGAGCATCAGCCAAAAGTAGCA
  CTGAGCACGTTGAGAGATCTCCAGTGCCCAGTGCTGCAGAGCAGCGAGCTGGAGGGAACGCCAGAGGTGT
  CCTGCCGGGCTCTGGAGCTCTTCGACTGGCTCGGCGCCGTCTTCAGTAATGTCGACCTAAATAATGAGCC
  TAATAATTTCATATCAACCTATTGCTGTCCTGAGCCAAGCACAGTGGTGGCAAAAGCTTATTTGTGTACA
  ATCACTGGCTTCATACTTCCAGAGAAGATCTGTCTCCTATTGGAACATCTCTGTCACTACTTTGATGAAC
  CGAAGTTAGCTCCATGGGTTACACTGTCCGTTCAAGGCTTTGCAGACAGCCCTGTTTCTTGGGAAAAAAA
  TGAACATGGTTTTCGAAAAGGAGGAGAACATTTATATAACTTTGTGATTTTTAATAATCAGGACTATTGG
  CTTCAGATGGCTGTTGGGGCAAATGATCACTGTCCACCA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219319 representing NM_006638
  Red=Cloning site Green=Tags(s)
  
  MATLRRLREAPRHLLVCEKSNFGNHKSRHRHLVQTHYYNYRVSFLIPECGILSEELKNLVMNTGPYYFVK
  NLPLHELITPEFISTFIKKGSCYALTYNTHIDEDNTVALLPNGKLILSLDKDTYEETGLQGHPSQFSGRK
  IMKFIVSIDLMELSLNLDSKKYERISWSFKEKKPLKFDFLLAWHKTGSEESTMMSYFSKYQIQEHQPKVA
  LSTLRDLQCPVLQSSELEGTPEVSCRALELFDWLGAVFSNVDLNNEPNNFISTYCCPEPSTVVAKAYLCT
  ITGFILPEKICLLLEHLCHYFDEPKLAPWVTLSVQGFADSPVSWEKNEHGFRKGGEHLYNFVIFNNQDYW
  LQMAVGANDHCPP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006638
• ORF Size: 1089 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006638.2, NM_006638.3, NP_006629.2
• RefSeq Size: 1174 bp
• RefSeq ORF: 1092 bp
• Locus ID: 10799
• Protein Families: Stem cell - Pluripotency
• MW: 41.7 kDa
• Gene Summary: Component of ribonuclease P, a protein complex that generates mature tRNA molecules by cleaving their 5'-ends. [UniProtKB/Swiss-Prot Function]