CDC14A (NM_003672) Human Tagged ORF Clone

CAT#: RC219324

CDC14A (Myc-DDK-tagged)-Human CDC14 cell division cycle 14 homolog A (S. cerevisiae) (CDC14A), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY418511) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219324 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CDC14A
• Synonyms: cdc14; DFNB32; DFNB35; DFNB105; hCDC14
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219324 representing NM_003672
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCGGAGTCAGGGGAACTAATCGGGGCTTGTGAGTTCATGAAAGATCGGTTATATTTTGCTACTT
  TAAGGAATAGACCAAAAAGCACAGTAAATACCCACTATTTCTCCATCGATGAGGAGCTGGTCTATGAAAA
  TTTCTATGCAGATTTTGGACCGCTGAACTTGGCAATGGTGTACAGATATTGCTGCAAACTAAACAAGAAA
  CTAAAATCATACAGTTTGTCAAGAAAGAAAATAGTGCACTACACCTGTTTTGACCAACGGAAAAGAGCAA
  ATGCAGCATTTTTGATAGGTGCCTATGCAGTAATCTATTTAAAGAAGACACCAGAAGAAGCCTACAGAGC
  ACTCCTGTCTGGCTCAAACCCCCCCTATCTTCCATTCAGGGATGCTTCCTTTGGAAATTGCACTTACAAT
  CTCACCATTCTCGACTGTTTGCAGGGAATCAGAAAGGGATTACAACATGGATTTTTTGACTTTGAGACAT
  TTGATGTGGATGAATATGAACATTATGAGCGAGTTGAAAATGGTGACTTCAACTGGATTGTTCCAGGAAA
  ATTTTTAGCATTTAGTGGACCACATCCTAAAAGCAAAATTGAGAATGGTTATCCTCTTCACGCCCCTGAA
  GCCTACTTTCCTTATTTCAAAAAGCATAATGTGACTGCAGTTGTGAGGCTAAACAAAAAGATTTATGAGG
  CAAAGCGCTTCACAGACGCTGGCTTCGAGCACTATGACCTCTTCTTCATAGATGGCAGCACACCCAGTGA
  CAACATCGTGCGAAGGTTCCTGAACATCTGTGAGAACACCGAAGGGGCCATCGCCGTTCACTGCAAAGCT
  GGTCTTGGAAGAACAGGGACATTGATAGCCTGTTATGTAATGAAACACTACAGGTTTACACATGCTGAAA
  TAATTGCTTGGATTAGAATATGCCGGCCAGGCTCTATTATAGGACCCCAGCAGCACTTCCTGGAAGAAAA
  ACAAGCATCGTTGTGGGTCCAAGGAGACATTTTCCGATCCAAACTGAAAAATCGACCATCCAGTGAAGGA
  AGTATTAATAAAATTCTTTCTGGCCTAGATGATATGTCTATTGGTGGAAATCTTTCAAAAACACAAAACA
  TGGAACGATTTGGAGAGGATAACTTAGAAGATGATGATGTGGAAATGAAAAATGGTATAACCCAGGGAGA
  CAAACTACGTGCCTTAAAAAGTCAGAGACAGCCACGTACCTCACCATCCTGTGCATTTAGGTCAGATGAT
  ACAAAAGGACATCCAAGAGCAGTGTCCCAGCCTTTCAGATTAAGTTCATCCCTGCAAGGATCTGCAGTTA
  CTTTGAAGACATCAAAAATGGCACTGTCCCCTTCAGCAACGGCCAAGAGGATCAACAGAACTTCTTTGTC
  TTCGGGTGCCACTGTAAGAAGCTTTTCCATAAACTCCCGGCTAGCCAGTTCTCTAGGGAACTTGAATGCT
  GCAACAGATGATCCAGAGAACAAAAAGACCTCCTCATCCTCTAAGGCAGGCTTCACAGCCAGCCCGTTTA
  CCAACCTCTTGAATGGCAGCTCCCAGCCAACTACCAGAAATTACCCTGAGCTCAACAATAATCAGTACAA
  CAGAAGCAGCAACAGCAACGGGGGCAACCTGAACAGCCCCCCAGGCCCCCACAGCGCCAAGACAGAGGAG
  CACACCACCATCCTCCGACCCTCCTACACCGGGCTTTCTTCTTCTTCAGCGAGATTCCTGAGCCGTTCTA
  TCCCTTCCCTTCAGTCTGAATATGTTCATTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219324 representing NM_003672
  Red=Cloning site Green=Tags(s)
  
  MAAESGELIGACEFMKDRLYFATLRNRPKSTVNTHYFSIDEELVYENFYADFGPLNLAMVYRYCCKLNKK
  LKSYSLSRKKIVHYTCFDQRKRANAAFLIGAYAVIYLKKTPEEAYRALLSGSNPPYLPFRDASFGNCTYN
  LTILDCLQGIRKGLQHGFFDFETFDVDEYEHYERVENGDFNWIVPGKFLAFSGPHPKSKIENGYPLHAPE
  AYFPYFKKHNVTAVVRLNKKIYEAKRFTDAGFEHYDLFFIDGSTPSDNIVRRFLNICENTEGAIAVHCKA
  GLGRTGTLIACYVMKHYRFTHAEIIAWIRICRPGSIIGPQQHFLEEKQASLWVQGDIFRSKLKNRPSSEG
  SINKILSGLDDMSIGGNLSKTQNMERFGEDNLEDDDVEMKNGITQGDKLRALKSQRQPRTSPSCAFRSDD
  TKGHPRAVSQPFRLSSSLQGSAVTLKTSKMALSPSATAKRINRTSLSSGATVRSFSINSRLASSLGNLNA
  ATDDPENKKTSSSSKAGFTASPFTNLLNGSSQPTTRNYPELNNNQYNRSSNSNGGNLNSPPGPHSAKTEE
  HTTILRPSYTGLSSSSARFLSRSIPSLQSEYVHY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003672
• ORF Size: 1782 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003672.2, NM_003672.3, NP_003663.2
• RefSeq Size: 4262
• RefSeq ORF: 1785
• Locus ID: 8556
• Domains: Y_phosphatase, DSPc, PTPc_motif
• Protein Families: Druggable Genome, Phosphatase
• Protein Pathways: Cell cycle
• MW: 66.4 kDa
• Gene Summary: The protein encoded by this gene is a member of the dual specificity protein tyrosine phosphatase family. It is highly similar to Saccharomyces cerevisiae Cdc14, a protein tyrosine phosphatase involved in the exit of cell mitosis and initiation of DNA replication, suggesting a role in cell cycle control. This protein has been shown to interact with, and dephosphorylate tumor suppressor protein p53, and is thought to regulate the function of p53. Alternative splicing of this gene results in several transcript variants encoding distinct isoforms. [provided by RefSeq, Jul 2008]