TAF15 (NM_003487) Human Tagged ORF Clone

CAT#: RC219341

TAF15 (Myc-DDK-tagged)-Human TAF15 RNA polymerase II, TATA box binding protein (TBP)-associated factor, 68kDa (TAF15), transcript variant 2. Note: ORF is codon optimized

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TAF15
• Synonyms: Npl3; RBP56; TAF2N; TAFII68
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219341 ORF sequence, codon optimized.
  Due to the complexity of NM_003487, the ORF clone is codon optimized for mammalian Expression.
  The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical.
  
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCTGACAGCGGATCCTACGGGCAAAGCGGGGGAGAACAACAAAGCTATTCCACTTATGGCAACCCCG
  GGTCACAGGGCTACGGCCAGGCTTCACAGTCATATAGCGGATATGGTCAGACAACGGACTCCTCATACGG
  CCAGAACTATAGCGGCTACTCTAGCTATGGCCAGTCTTACAGTCAGTCTTACGGGGGATACGAAAATCAG
  AAGCAGAGTAGCTACAGTCAGCAGCCGTACAACAACCAGGGACAGCAGCAGAACATGGAGAGCAGCGGGT
  CACAGGGAGGGCGGGCCCCTTCATATGACCAGCCCGATTATGGCCAGCAGGACAGCTATGATCAACAGAG
  CGGATACGATCAGCACCAGGGATCCTACGACGAGCAGAGCAATTACGATCAGCAGCACGACAGCTACAGC
  CAGAACCAGCAGAGCTATCACTCCCAACGCGAGAACTACTCCCACCACACTCAGGACGACCGGAGGGACG
  TTTCCCGCTACGGGGAAGACAACAGGGGCTATGGAGGCTCACAAGGAGGAGGTCGGGGCCGGGGAGGATA
  CGATAAGGATGGGCGAGGACCCATGACAGGCTCCTCTGGCGGGGACCGCGGCGGCTTCAAAAACTTCGGA
  GGGCACAGAGATTACGGACCACGAACTGATGCCGACTCAGAGAGTGATAACAGTGACAACAATACCATCT
  TTGTTCAAGGACTGGGGGAAGGCGTGTCCACAGATCAGGTTGGAGAGTTTTTCAAGCAAATCGGGATCAT
  TAAGACGAATAAGAAAACAGGGAAGCCAATGATCAATTTGTATACTGATAAGGACACCGGCAAGCCCAAA
  GGGGAGGCTACAGTATCATTTGATGACCCTCCATCCGCCAAGGCCGCCATCGATTGGTTCGACGGAAAGG
  AATTTCACGGCAACATCATTAAAGTTTCTTTTGCGACCAGGCGGCCTGAGTTTATGCGGGGTGGCGGATC
  TGGCGGGGGCCGAAGAGGGAGAGGAGGTTACCGGGGCCGCGGCGGCTTCCAGGGTCGAGGGGGCGATCCG
  AAAAGCGGCGACTGGGTCTGCCCCAACCCATCCTGCGGGAACATGAATTTTGCACGCCGGAACAGTTGCA
  ATCAGTGTAACGAACCCAGACCAGAAGATTCTAGGCCTAGCGGTGGTGACTTCCGAGGTCGCGGCTACGG
  AGGCGAGCGGGGGTATCGCGGCAGGGGCGGACGAGGGGGAGATCGCGGGGGCTATGGTGGCGACAGATCT
  GGAGGAGGCTACGGAGGCGATAGGAGCTCCGGAGGCGGCTACTCCGGCGACCGGTCAGGGGGCGGCTATG
  GCGGCGATCGATCTGGTGGAGGGTATGGCGGAGACAGAGGTGGAGGATATGGTGGGGATCGGGGTGGCGG
  ATATGGAGGAGATAGAGGCGGAGGCTATGGTGGGGACCGCGGCGGGTACGGTGGCGACCGAGGTGGCGGT
  TATGGAGGTGACCGCGGCGGATACGGTGGGGATCGGGGTGGTTACGGGGGGGATAGGGGAGGCTACGGCG
  GTGACAGAGGGGGCTACGGGGGGGATCGCAGCCGGGGAGGTTACGGCGGGGACAGAGGCGGGGGATCCGG
  GTACGGCGGTGATCGGTCCGGAGGATATGGCGGGGACCGATCTGGAGGGGGCTACGGCGGCGACAGAGGA
  GGCGGCTATGGGGGCGACAGGGGCGGATACGGAGGCAAAATGGGGGGGCGGAACGATTACCGGAACGATC
  AGAGGAACCGACCCTAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219341 representing NM_003487
  Red=Cloning site Green=Tags(s)
  
  MSDSGSYGQSGGEQQSYSTYGNPGSQGYGQASQSYSGYGQTTDSSYGQNYSGYSSYGQSYSQSYGGYENQ
  KQSSYSQQPYNNQGQQQNMESSGSQGGRAPSYDQPDYGQQDSYDQQSGYDQHQGSYDEQSNYDQQHDSYS
  QNQQSYHSQRENYSHHTQDDRRDVSRYGEDNRGYGGSQGGGRGRGGYDKDGRGPMTGSSGGDRGGFKNFG
  GHRDYGPRTDADSESDNSDNNTIFVQGLGEGVSTDQVGEFFKQIGIIKTNKKTGKPMINLYTDKDTGKPK
  GEATVSFDDPPSAKAAIDWFDGKEFHGNIIKVSFATRRPEFMRGGGSGGGRRGRGGYRGRGGFQGRGGDP
  KSGDWVCPNPSCGNMNFARRNSCNQCNEPRPEDSRPSGGDFRGRGYGGERGYRGRGGRGGDRGGYGGDRS
  GGGYGGDRSSGGGYSGDRSGGGYGGDRSGGGYGGDRGGGYGGDRGGGYGGDRGGGYGGDRGGYGGDRGGG
  YGGDRGGYGGDRGGYGGDRGGYGGDRGGYGGDRSRGGYGGDRGGGSGYGGDRSGGYGGDRSGGGYGGDRG
  GGYGGDRGGYGGKMGGRNDYRNDQRNRPY
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003487
• ORF Size: 1767 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_003487.1, NM_003487.2, NM_003487.3, NP_003478.1
• RefSeq Size: 2208
• RefSeq ORF: 1770
• Locus ID: 8148
• Protein Families: Druggable Genome
• MW: 61.6 kDa
• Gene Summary: This gene encodes a member of the TET family of RNA-binding proteins. The encoded protein plays a role in RNA polymerase II gene transcription as a component of a distinct subset of multi-subunit transcription initiation factor TFIID complexes. Translocations involving this gene play a role in acute leukemia and extraskeletal myxoid chondrosarcoma, and mutations in this gene may play a role in amyotrophic lateral sclerosis. Alternatively spliced transcript variants encoding multiple isoforms have been observed for this gene. [provided by RefSeq, May 2012]