CENPN (NM_001100625) Human Tagged ORF Clone

CAT#: RC219420

CENPN (Myc-DDK-tagged)-Human centromere protein N (CENPN), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY420287) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219420 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CENPN
• Synonyms: BM039; C16orf60; CENP-N; ICEN32
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219420 representing NM_001100625
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGAGACTGTTGCTGAGTTCATCAAGAGGACCATCTTGAAAATCCCCATGAATGAACTGACAACAA
  TCCTGAAGGCCTGGGATTTTTTGTCTGAAAATCAACTGCAGACTGTAAATTTCCGACAGAGAAAGGAATC
  TGTAGTTCAGCACTTGATCCATCTGTGTGAGGAAAAGCGTGCAAGTATCAGTGATGCTGCCCTGTTAGAC
  ATCATTTATATGCAATTTCATCAGCACCAGAAAGTTTGGGATGTTTTTCAGATGAGTAAAGGACCAGGTG
  AAGATGTTGACCTTTTTGATATGAAACAATTTAAAAATTCGTTCAAGAAAATTCTTCAGAGAGCATTAAA
  AAATGTGACAGTCAGCTTCAGAGAAACTGAGGAGAATGCAGTCTGGATTCGAATTGCCTGGGGAACACAG
  TACACAAAGCCAAACCAGTACAAACCTACCTACGTGGTGTACTACTCCCAGACTCCGTACGCCTTCACGT
  CCTCCTCCATGCTGAGGCGCAATACACCGCTTCTGGGTCAGGCGCTGACAATTGCTAGCAAACACCATCA
  GATTGTGAAAATGGACCTGAGAAGTCGGTATCTGGACTCTCTTAAGGCTATTGTTTTTAAACAGTATAAT
  CAGACCTTTGAAACTCACAACTCTACGACACCTCTACAGGAAAGAAGCCTTGGACTAGATATAAATATGG
  ATTCAAGGATCATTCATGAAAACATAGTAGAAAAAGAGAGAGTCCAACGAATAACTCAAGAAACATTTGG
  AGATTATCCTCAACCACAACTAGAATTTGCACAATATAAGCTTGAAACGAAATTCAAAAGTGGTTTAAAT
  GGGAGCATCTTGGCTGAGAGGGAAGAACCCCTCCGATGCCTAATAAAGTTCTCTAGCCCACATCTTCTGG
  AAGCATTGAAATCCTTAGCACCAGCGGCACTTGTTTGCAGGATCCAAAAGCTGCTCTGCTATTCTGGAAG
  CCACAGTCAAGGGACCCAGGACCCGAGCAGCTGGCAGAAGGACTTGTACCTTCTGTTTGTCCCATTGTAT
  CCAAGATGT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219420 representing NM_001100625
  Red=Cloning site Green=Tags(s)
  
  MDETVAEFIKRTILKIPMNELTTILKAWDFLSENQLQTVNFRQRKESVVQHLIHLCEEKRASISDAALLD
  IIYMQFHQHQKVWDVFQMSKGPGEDVDLFDMKQFKNSFKKILQRALKNVTVSFRETEENAVWIRIAWGTQ
  YTKPNQYKPTYVVYYSQTPYAFTSSSMLRRNTPLLGQALTIASKHHQIVKMDLRSRYLDSLKAIVFKQYN
  QTFETHNSTTPLQERSLGLDINMDSRIIHENIVEKERVQRITQETFGDYPQPQLEFAQYKLETKFKSGLN
  GSILAEREEPLRCLIKFSSPHLLEALKSLAPAALVCRIQKLLCYSGSHSQGTQDPSSWQKDLYLLFVPLY
  PRC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001100625
• ORF Size: 1059 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001100625.1, NM_001100625.2, NP_001094095.1, NP_001094095.2
• RefSeq Size: 2265
• RefSeq ORF: 1062
• Locus ID: 55839
• Protein Families: Druggable Genome
• MW: 41 kDa
• Gene Summary: The protein encoded by this gene forms part of the nucleosome-associated complex and is important for kinetochore assembly. It is bound to kinetochores during S phase and G2 and recruits other proteins to the centromere. Pseudogenes of this gene are located on chromosome 2. Alternative splicing results in multiple transcript variants that encode different protein isoforms. [provided by RefSeq, Jul 2012]