Kv4.3 (KCND3) (NM_172198) Human Tagged ORF Clone

CAT#: RC219447

KCND3 (Myc-DDK-tagged)-Human potassium voltage-gated channel, Shal-related subfamily, member 3 (KCND3), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY401549) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222757 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: KCND3
• Synonyms: BRGDA9; KCND3L; KCND3S; KSHIVB; KV4.3; SCA19; SCA22
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219447 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGGCCGGAGTTGCGGCCTGGCTGCCTTTTGCCCGGGCTGCGGCCATCGGGTGGATGCCGGTGGCCA
  ACTGCCCCATGCCCCTGGCCCCGGCCGACAAGAACAAGCGGCAGGATGAGCTGATTGTCCTCAACGTGAG
  TGGGCGGAGGTTCCAGACCTGGAGGACCACGCTGGAGCGCTACCCGGACACCCTGCTGGGCAGCACGGAG
  AAGGAGTTCTTCTTCAACGAGGACACCAAGGAGTACTTCTTCGACCGGGACCCCGAGGTGTTCCGCTGCG
  TGCTCAACTTCTACCGCACGGGGAAGCTGCACTACCCGCGCTACGAGTGCATCTCTGCCTACGACGACGA
  GCTGGCCTTCTACGGCATCCTCCCGGAGATCATCGGGGACTGCTGCTACGAGGAGTACAAGGACCGCAAG
  AGGGAGAACGCCGAGCGGCTCATGGACGACAACGACTCGGAGAACAACCAGGAGTCCATGCCCTCGCTCA
  GCTTCCGCCAGACCATGTGGCGGGCCTTCGAGAACCCCCACACCAGCACGCTGGCCCTGGTCTTCTACTA
  CGTGACTGGCTTCTTCATCGCTGTCTCGGTCATCACCAACGTGGTGGAGACGGTGCCGTGCGGCACGGTC
  CCGGGCAGCAAGGAGCTGCCGTGCGGGGAGCGCTACTCGGTGGCCTTCTTCTGCCTGGACACGGCGTGCG
  TCATGATCTTCACCGTGGAGTACCTCCTGCGGCTCTTCGCGGCTCCCAGCCGCTACCGCTTCATCCGCAG
  CGTCATGAGCATCATCGACGTGGTGGCCATCATGCCCTACTACATCGGTCTGGTCATGACCAACAACGAG
  GACGTGTCCGGCGCCTTCGTCACGCTCCGGGTCTTCCGCGTCTTCAGGATCTTCAAGTTTTCCCGCCACT
  CCCAGGGCCTGCGGATCCTGGGCTACACACTGAAGAGCTGTGCCTCCGAACTGGGCTTTCTTCTCTTCTC
  CCTCACCATGGCCATCATCATCTTTGCCACTGTGATGTTTTATGCCGAGAAGGGCTCCTCGGCCAGCAAG
  TTCACAAGCATCCCTGCCTCGTTTTGGTACACCATTGTCACCATGACCACACTGGGATACGGAGACATGG
  TGCCTAAGACGATTGCAGGGAAGATCTTCGGCTCCATCTGCTCCTTGAGTGGCGTCCTGGTCATTGCCCT
  GCCAGTCCCTGTGATTGTTTCCAACTTTAGCCGGATTTACCACCAGAATCAGAGAGCTGATAAACGCAGG
  GCACAAAAGAAGGCCCGCCTTGCCAGGATCCGTGTGGCCAAAACAGGCAGTTCGAATGCATACCTGCACA
  GCAAGCGCAACGGGCTCCTCAACGAGGCGCTGGAGCTGACGGGCACCCCAGAAGAGGAGCACATGGGCAA
  GACCACCTCACTCATCGAGAGCCAGCATCATCACCTGCTGCACTGCCTGGAAAAAACCACTAACCACGAG
  TTTATTGATGAGCAGATGTTTGAGCAGAACTGCATGGAGAGTTCAATGCAGAACTACCCATCCACAAGAA
  GTCCCTCACTGTCCAGCCACCCAGGCCTCACTACCACCTGCTGCTCCCGTCGTAGTAAGAAGACCACACA
  CCTGCCCAATTCTAACCTGCCAGCTACTCGCCTGCGCAGCATGCAAGAGCTCAGCACGATCCACATCCAG
  GGCAGTGAGCAGCCCTCCCTCACAACCAGTCGCTCCAGCCTTAATTTGAAAGCAGACGACGGACTGAGAC
  CAAACTGCAAAACATCCCAGATCACCACAGCCATCATCAGCATCCCCACTCCCCCAGCGCTAACCCCAGA
  GGGGGAAAGTCGGCCACCCCCTGCCAGCCCAGGCCCCAACACGAACATTCCTTCCATAGCCAGCAATGTT
  GTCAAGGTCTCCGCCTTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219447 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MAAGVAAWLPFARAAAIGWMPVANCPMPLAPADKNKRQDELIVLNVSGRRFQTWRTTLERYPDTLLGSTE
  KEFFFNEDTKEYFFDRDPEVFRCVLNFYRTGKLHYPRYECISAYDDELAFYGILPEIIGDCCYEEYKDRK
  RENAERLMDDNDSENNQESMPSLSFRQTMWRAFENPHTSTLALVFYYVTGFFIAVSVITNVVETVPCGTV
  PGSKELPCGERYSVAFFCLDTACVMIFTVEYLLRLFAAPSRYRFIRSVMSIIDVVAIMPYYIGLVMTNNE
  DVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLLFSLTMAIIIFATVMFYAEKGSSASK
  FTSIPASFWYTIVTMTTLGYGDMVPKTIAGKIFGSICSLSGVLVIALPVPVIVSNFSRIYHQNQRADKRR
  AQKKARLARIRVAKTGSSNAYLHSKRNGLLNEALELTGTPEEEHMGKTTSLIESQHHHLLHCLEKTTNHE
  FIDEQMFEQNCMESSMQNYPSTRSPSLSSHPGLTTTCCSRRSKKTTHLPNSNLPATRLRSMQELSTIHIQ
  GSEQPSLTTSRSSLNLKADDGLRPNCKTSQITTAIISIPTPPALTPEGESRPPPASPGPNTNIPSIASNV
  VKVSAL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_172198
• ORF Size: 1908 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_172198.1, NM_172198.2, NP_751948.1
• RefSeq Size: 2636 bp
• RefSeq ORF: 1911 bp
• Locus ID: 3752
• Cytogenetics: 1p13.2
• Protein Families: Druggable Genome, Ion Channels: Potassium, Transmembrane
• MW: 71.4 kDa
• Gene Summary: 'Voltage-gated potassium (Kv) channels represent the most complex class of voltage-gated ion channels from both functional and structural standpoints. Their diverse functions include regulating neurotransmitter release, heart rate, insulin secretion, neuronal excitability, epithelial electrolyte transport, smooth muscle contraction, and cell volume. Four sequence-related potassium channel genes - shaker, shaw, shab, and shal - have been identified in Drosophila, and each has been shown to have human homolog(s). This gene encodes a member of the potassium channel, voltage-gated, shal-related subfamily, members of which form voltage-activated A-type potassium ion channels and are prominent in the repolarization phase of the action potential. This member includes two isoforms with different sizes, which are encoded by alternatively spliced transcript variants of this gene. [provided by RefSeq, Jul 2008]'