DGKI (NM_004717) Human Tagged ORF Clone

CAT#: RC219587

DGKI (Myc-DDK-tagged)-Human diacylglycerol kinase, iota (DGKI)

Product Images

Western blot validation of overexpression lysate (Cat# LY417800) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219587 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified DGKI protein (Cat# TP319587). The protein was produced from HEK293T cells transfected with DGKI cDNA clone (Cat# RC219587) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: DGKI
• Synonyms: DGK-IOTA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219587 representing NM_004717
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGATGCTGCGGGAAGGGGCTGCCATTTGCTGCCCCTGCCAGCGGCGCGCGGACCTGCCCGCGCTCCTG
  CAGCCGCCGCCGCCGCCGCCGCCAGCCCGCCCGGCCCCTGCAGCGGCGCCGCCTGCGCTCCCTCCGCGGC
  CGCCGGAGCGGGCGCCATGAACCCCAGCTCCTCGGCGGGAGAGGAGAAAGGGGCGACGGGCGGCAGCAGC
  AGCAGCGGAAGCGGCGCCGGGAGCTGCTGCCTGGGCGCCGAGGGCGGCGCGGACCCGCGGGGCGCAGGGT
  CAGCCGCGGCGGCGGGGGCCGCTGCCCTGGACGAGCCCGCGGCCGCCGGCCAGAAGGAGAAGGACGAAGC
  GCTGGAGGAGAAGCTGAGGAACTTAACTTTCCGGAAGCAGGTCTCGTACAGGAAAGCAATCTCCCGGGCA
  GGCCTCCAGCATCTGGCTCCTGCACATCCCCTCAGCCTTCCTGTGGCAAATGGTCCAGCCAAGGAGCCCA
  GAGCGACTTTGGACTGGAGTGAGAATGCCGTGAATGGAGAACACCTGTGGCTGGAGACCAACGTCTCGGG
  AGACCTCTGCTACCTTGGAGAGGAGAACTGCCAAGTCAGATTTGCAAAATCAGCTCTCAGGAGGAAGTGT
  GCAGTCTGTAAAATCGTCGTCCACACCGCCTGCATTGAGCAGCTAGAAAAGATTAATTTCAGATGTAAAC
  CAACATTTCGAGAAGGAGGCTCAAGGTCACCAAGAGAAAATTTTGTACGTCATCACTGGGTGCACAGGCG
  TCGGCAGGAGGGGAAATGTAAGCAGTGTGGTAAGGGCTTCCAGCAAAAGTTCTCCTTCCACAGTAAAGAG
  ATTGTGGCTATCAGCTGTTCCTGGTGCAAGCAGGCGTTTCACAATAAGGTGACCTGCTTCATGCTGCATC
  ACATTGAAGAACCCTGCTCCCTGGGGGCTCATGCTGCTGTTATTGTCCCGCCCACTTGGATCATTAAGGT
  GAAGAAACCTCAGAACTCCCTGAAGGCTTCAAATCGGAAGAAGAAGAGAACAAGCTTTAAAAGAAAAGCC
  AGTAAAAGAGGGATGGAACAGGAAAACAAAGGTCGTCCTTTTGTGATAAAACCCATCTCTTCTCCTCTCA
  TGAAACCCTTGCTTGTATTTGTGAATCCCAAGAGTGGAGGCAACCAGGGAACCAAAGTCCTGCAGATGTT
  CATGTGGTACCTGAATCCACGGCAAGTCTTTGATCTTTCTCAGGAAGGGCCAAAAGATGCGCTTGAATTG
  TATAGGAAAGTACCAAATCTGCGAATTCTGGCCTGTGGTGGGGATGGAACGGTGGGCTGGATCCTTTCCA
  TCCTGGATGAACTGCAGCTGAGCCCTCAGCCTCCTGTGGGGGTCCTTCCTCTGGGGACTGGGAATGACCT
  GGCTCGAACTCTCAACTGGGGAGGGGGCTACACTGATGAACCTGTTTCTAAGATCCTGTGTCAAGTGGAA
  GATGGGACAGTTGTACAGCTAGATCGCTGGAACCTCCATGTGGAAAGAAACCCCGACTTGCCTCCAGAAG
  AACTTGAAGATGGCGTATGTAAGCTCCCTCTGAATGTTTTCAATAACTACTTCAGCCTTGGATTTGATGC
  CCATGTCACACTGGAGTTCCATGAATCCAGAGAAGCAAATCCAGAGAAATTCAACAGTCGTTTTCGAAAT
  AAAATGTTCTATGCAGGGGCAGCTTTTTCTGACTTCCTACAGAGAAGTTCTAGAGATCTATCCAAACATG
  TTAAAGTTGTTTGTGATGGAACAGATCTCACCCCAAAGATTCAGGAACTGAAGTTCCAGTGTATAGTATT
  TTTAAATATACCCAGATATTGTGCTGGCACAATGCCCTGGGGAAACCCAGGTGATCACCATGATTTCGAA
  CCTCAGCGTCATGATGATGGTTATATTGAAGTCATTGGATTTACCATGGCCTCTTTGGCAGCCCTGCAAG
  TTGGGGGCCATGGAGAGAGGCTACACCAGTGTCGAGAAGTCATGCTTCTAACTTACAAATCCATCCCCAT
  GCAAGTGGATGGGGAGCCCTGTAGGTTGGCCCCAGCTATGATTCGGATCTCCCTGAGGAATCAGGCCAAC
  ATGGTACAGAAGAGCAAGAGGAGAACATCCATGCCTTTACTCAATGATCCCCAGTCTGTCCCAGATCGTC
  TGAGGATCCGGGTGAACAAAATCAGTTTACAAGACTATGAAGGATTCCACTATGACAAGGAGAAACTCCG
  AGAAGCTTCTATTTCAGACTGGTTAAGAACCATTGCTGGGGAACTAGTGCAGTCATTTGGAGCGATACCT
  CTGGGTATTCTAGTTGTGCGTGGAGACTGTGATTTGGAGACTTGCCGTATGTACATAGACCGCCTACAGG
  AGGACCTACAGTCAGTTTCTTCTGGCTCCCAGAGAGTTCATTACCAGGACCATGAAACCTCCTTCCCCAG
  GGCTCTCTCAGCACAGAGGCTCTCTCCTCGGTGGTGCTTCCTAGATGACAGATCTCAGGAACATTTGCAC
  TTTGTGATGGAGATTTCCCAAGATGAGATTTTTATTCTGGACCCAGATATGGTGGTGTCACAGCCGGCGG
  GGACACCTCCGGGCATGCCTGACCTGGTGGTGGAACAAGCCTCGGGGATCTCAGACTGGTGGAATCCTGC
  CCTGCGGAAACGCATGCTGAGTGACAGTGGGCTGGGGATGATAGCTCCCTATTATGAGGACTCAGATCTG
  AAAGATCTCAGCCACTCCCGCGTGCTACAGTCACCAGTCTCTTCAGAAGATCATGCAATTTTGCAGGCAG
  TAATAGCTGGTGATCTTATGAAGCTAATAGAAAGCTATAAAAATGGAGGCAGTCTGCTAATTCAGGGACC
  AGACCACTGTTCACTCCTTCACTACGCAGCTAAAACCGGCAACGGGGAGATTGTGAAATATATCCTTGAC
  CACGGACCTTCCGAGTTATTGGATATGGCAGACAGTGAAACGGGTGAGACTGCACTGCACAAGGCTGCCT
  GCCAGCGGAACCGGGCTGTGTGCCAGCTTCTGGTGGATGCAGGAGCATCTCTGAGAAAGACGGACTCCAA
  GGGTAAGACACCTCAAGAAAGAGCACAGCAGGCTGGGGACCCAGACTTGGCTGCTTACCTAGAAAGCCGT
  CAGAACTATAAGGTCATTGGCCATGAGGACCTGGAAACTGCTGTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219587 representing NM_004717
  Red=Cloning site Green=Tags(s)
  
  MDAAGRGCHLLPLPAARGPARAPAAAAAAAASPPGPCSGAACAPSAAAGAGAMNPSSSAGEEKGATGGSS
  SSGSGAGSCCLGAEGGADPRGAGSAAAAGAAALDEPAAAGQKEKDEALEEKLRNLTFRKQVSYRKAISRA
  GLQHLAPAHPLSLPVANGPAKEPRATLDWSENAVNGEHLWLETNVSGDLCYLGEENCQVRFAKSALRRKC
  AVCKIVVHTACIEQLEKINFRCKPTFREGGSRSPRENFVRHHWVHRRRQEGKCKQCGKGFQQKFSFHSKE
  IVAISCSWCKQAFHNKVTCFMLHHIEEPCSLGAHAAVIVPPTWIIKVKKPQNSLKASNRKKKRTSFKRKA
  SKRGMEQENKGRPFVIKPISSPLMKPLLVFVNPKSGGNQGTKVLQMFMWYLNPRQVFDLSQEGPKDALEL
  YRKVPNLRILACGGDGTVGWILSILDELQLSPQPPVGVLPLGTGNDLARTLNWGGGYTDEPVSKILCQVE
  DGTVVQLDRWNLHVERNPDLPPEELEDGVCKLPLNVFNNYFSLGFDAHVTLEFHESREANPEKFNSRFRN
  KMFYAGAAFSDFLQRSSRDLSKHVKVVCDGTDLTPKIQELKFQCIVFLNIPRYCAGTMPWGNPGDHHDFE
  PQRHDDGYIEVIGFTMASLAALQVGGHGERLHQCREVMLLTYKSIPMQVDGEPCRLAPAMIRISLRNQAN
  MVQKSKRRTSMPLLNDPQSVPDRLRIRVNKISLQDYEGFHYDKEKLREASISDWLRTIAGELVQSFGAIP
  LGILVVRGDCDLETCRMYIDRLQEDLQSVSSGSQRVHYQDHETSFPRALSAQRLSPRWCFLDDRSQEHLH
  FVMEISQDEIFILDPDMVVSQPAGTPPGMPDLVVEQASGISDWWNPALRKRMLSDSGLGMIAPYYEDSDL
  KDLSHSRVLQSPVSSEDHAILQAVIAGDLMKLIESYKNGGSLLIQGPDHCSLLHYAAKTGNGEIVKYILD
  HGPSELLDMADSETGETALHKAACQRNRAVCQLLVDAGASLRKTDSKGKTPQERAQQAGDPDLAAYLESR
  QNYKVIGHEDLETAV
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004717
• ORF Size: 3195 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004717.1, NM_004717.2, NM_004717.3, NP_004708.1
• RefSeq Size: 4780
• RefSeq ORF: 3198
• Locus ID: 9162
• Domains: DAGKa, DAGKc, ANK, DAG_PE-bind
• Protein Families: Druggable Genome
• Protein Pathways: Glycerolipid metabolism, Glycerophospholipid metabolism, Metabolic pathways, Phosphatidylinositol signaling system
• MW: 116.8 kDa
• Gene Summary: This gene is a member of the type IV diacylglycerol kinase subfamily. Diacylglycerol kinases regulate the intracellular concentration of diacylglycerol through its phosphorylation, producing phosphatidic acid. The specific role of the enzyme encoded by this gene is undetermined, however, it may play a crucial role in the production of phosphatidic acid in the retina or in recessive forms of retinal degeneration. [provided by RefSeq, Jul 2008]