OLAH (NM_018324) Human Tagged ORF Clone

CAT#: RC219627

OLAH (Myc-DDK-tagged)-Human oleoyl-ACP hydrolase (OLAH), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY413121) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219627 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OLAH
• Synonyms: AURA1; SAST; TE2; THEDC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219627 representing NM_018324
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGAGAGGAGACCAACCTAAGAGAACCAGGAATGAAAACATTTTCAACTGCTTATACAAAAACCCTG
  AGGCAACTTTTAAGCTGATTTGCTTTCCCTGGATGGGAGGTGGCTCCACTCATTTTGCCAAATGGGGCCA
  AGATACTCATGATTTGCTGGAAGAGACAGCATCTCACCATGTTGCCAAGGCTGGTCTCAAACTCCGGCGC
  TCAAGTGATCCTCCTGCTTCAGCCTACCCATGTGCTGGCGTGAGCCACCGTAGGCGTGAGCCACCGTGCC
  TGGCCAAAATTCTTGGTCTATTCTGGATTCTAATTTTTTTTATGCACTCCTTAAGGCTTCCTGGAAGAGA
  AAGCAGAGTTGAAGAACCTCTTGAAAATGACATCTCCCAGTTAGTTGATGAAGTTGTTTGTGCTCTGCAG
  CCAGTCATCCAGGATAAACCATTTGCATTTTTTGGCCACAGTATGGGATCCTACATTGCTTTTAGGACTG
  CACTAGGTCTAAAAGAAAACAATCAACCAGAACCATTGCATTTATTTTTGTCAAGTGCAACTCCTGTACA
  TTCAAAGGCCTGGCATCGCATTCCCAAAGATGATGAATTGTCAGAAGAACAAATAAGTCATTACCTTATG
  GAATTTGGAGGCACCCCCAAGCATTTTGCTGAAGCCAAGGAATTTGTGAAACAATGTAGTCCCATCATAA
  GGGCAGATCTGAACATTGTTAGAAGTTGCACCTCTAACGTACCATCTAAGGCTGTTCTTTCCTGTGACTT
  GACATGTTTTGTTGGATCTGAAGACATAGCAAAGGACATGGAAGCCTGGAAAGATGTAACCAGTGGAAAT
  GCTAAAATTTACCAGCTTCCAGGGGGTCACTTTTATCTTCTGGATCCTGCGAACGAGAAATTAATCAAGA
  ACTACATAATCAAGTGTCTAGAAGTATCATCGATATCCAATTTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219627 representing NM_018324
  Red=Cloning site Green=Tags(s)
  
  MERGDQPKRTRNENIFNCLYKNPEATFKLICFPWMGGGSTHFAKWGQDTHDLLEETASHHVAKAGLKLRR
  SSDPPASAYPCAGVSHRRREPPCLAKILGLFWILIFFMHSLRLPGRESRVEEPLENDISQLVDEVVCALQ
  PVIQDKPFAFFGHSMGSYIAFRTALGLKENNQPEPLHLFLSSATPVHSKAWHRIPKDDELSEEQISHYLM
  EFGGTPKHFAEAKEFVKQCSPIIRADLNIVRSCTSNVPSKAVLSCDLTCFVGSEDIAKDMEAWKDVTSGN
  AKIYQLPGGHFYLLDPANEKLIKNYIIKCLEVSSISNF
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018324
• ORF Size: 954 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018324.1, NM_018324.2, NP_060794.1
• RefSeq Size: 1765
• RefSeq ORF: 957
• Locus ID: 55301
• Protein Pathways: Fatty acid biosynthesis, Metabolic pathways
• MW: 35.6 kDa