Mesothelin (MSLN) (NM_013404) Human Tagged Lenti ORF Clone
CAT#: RC219757L1
- LentiORF®
Lenti ORF clone of Human mesothelin (MSLN), transcript variant 2, Myc-DDK-tagged
"NM_013404" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | Mesothelin |
Synonyms | MPF; SMRP |
Vector | pLenti-C-Myc-DDK |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | None |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC219757).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene |
ACCN | NM_013404 |
ORF Size | 1890 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_013404.3 |
RefSeq Size | 2076 bp |
RefSeq ORF | 1893 bp |
Locus ID | 10232 |
UniProt ID | Q13421 |
Cytogenetics | 16p13.3 |
Protein Families | Druggable Genome, Secreted Protein, Transmembrane |
MW | 68.99 kDa |
Gene Summary | This gene encodes a preproprotein that is proteolytically processed to generate two protein products, megakaryocyte potentiating factor and mesothelin. Megakaryocyte potentiating factor functions as a cytokine that can stimulate colony formation of bone marrow megakaryocytes. Mesothelin is a glycosylphosphatidylinositol-anchored cell-surface protein that may function as a cell adhesion protein. This protein is overexpressed in epithelial mesotheliomas, ovarian cancers and in specific squamous cell carcinomas. Alternative splicing results in multiple transcript variants, at least one of which encodes an isoform that is proteolytically processed. [provided by RefSeq, Feb 2016] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC304006 | MSLN (untagged)-Human mesothelin (MSLN), transcript variant 2 |
USD 1,070.00 |
|
RC219757 | MSLN (Myc-DDK-tagged)-Human mesothelin (MSLN), transcript variant 2 |
USD 98.00 |
|
RG219757 | MSLN (GFP-tagged) - Human mesothelin (MSLN), transcript variant 2 |
USD 460.00 |
|
RC219757L2 | Lenti ORF clone of Human mesothelin (MSLN), transcript variant 2, mGFP tagged |
USD 620.00 |
|
RC219757L3 | Lenti ORF clone of Human mesothelin (MSLN), transcript variant 2, Myc-DDK-tagged |
USD 620.00 |
|
RC219757L4 | Lenti ORF clone of Human mesothelin (MSLN), transcript variant 2, mGFP tagged |
USD 620.00 |
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