CNO6L (CNOT6L) (NM_144571) Human Tagged ORF Clone

CAT#: RC219766

CNOT6L (Myc-DDK-tagged)-Human CCR4-NOT transcription complex, subunit 6-like (CNOT6L)

Product Images

Western blot validation of overexpression lysate (Cat# LY408280) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219766 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CNOT6L protein (Cat# TP319766). The protein was produced from HEK293T cells transfected with CNOT6L cDNA clone (Cat# RC219766) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CNOT6L
• Synonyms: CCR4b
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219766 representing NM_144571
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGACTAATAGGGATGCCAAAGGAAAAATATGATCCTCCAGATCCTCGCAGAATTTATACCATCATGT
  CAGCAGAGGAGGTAGCCAATGGGAAAAAATCTCACTGGGCAGAATTAGAAATCTCGGGTAGAGTGCGGAG
  CCTAAGTACATCACTTTGGTCATTGACACACTTGACAGCGCTGCACCTAAATGACAATTACCTTAGTCGC
  ATTCCACCTGATATTGCCAAGCTTCATAATCTGGTTTACCTGGATCTGTCATCCAATAAACTCAGAAGTT
  TACCAGCAGAACTAGGAAACATGGTGTCTCTCAGGGAATTGCTTTTAAATAACAATCTGTTACGGGTTTT
  GCCTTATGAACTTGGTCGGCTCTTCCAGCTACAAACTCTAGGTTTGAAAGGCAATCCTTTATCACAGGAT
  ATTCTCAACTTATACCAGGACCCAGATGGAACCCGAAAGCTACTGAACTTCATGCTTGACAATCTCGCAG
  TTCATCCAGAGCAGCTTCCTCCGAGGCCATGGATTACATTAAAAGAACGAGACCAAATTCTGCCGTCAGC
  ATCATTCACGGTTATGTGTTACAATGTGTTATGTGATAAATACGCTACCCGGCAGCTATATGGCTATTGC
  CCATCCTGGGCATTAAACTGGGAATACAGGAAAAAGGGAATTATGGAAGAAATTGTTAACTGTGACGCAG
  ATATCATTAGTCTTCAGGAAGTGGAAACAGAGCAATACTTCACTCTCTTTCTGCCAGCATTGAAGGAGCG
  TGGATATGATGGATTTTTTTCTCCAAAGTCACGTGCCAAAATCATGTCTGAGCAGGAGAGAAAGCATGTA
  GATGGTTGTGCAATATTCTTCAAAACAGAAAAATTTACATTGGTGCAGAAGCATACAGTGGAATTTAACC
  AAGTGGCGATGGCTAATTCAGATGGATCCGAAGCTATGCTGAACAGAGTGATGACAAAAGATAACATTGG
  TGTCGCTGTGGTATTAGAGGTCCACAAAGAACTATTTGGAGCAGGTATGAAGCCTATTCATGCTGCAGAC
  AAACAGCTGCTTATAGTGGCAAATGCCCACATGCATTGGGACCCAGAGTATTCTGATGTGAAGCTCATCC
  AGACCATGATGTTTGTCTCAGAGGTTAAAAACATTCTGGAGAAAGCCTCTAGTAGGCCTGGAAGCCCAAC
  TGCAGATCCTAATTCCATCCCGCTGGTGCTATGTGCAGATCTTAACTCATTGCCAGATTCAGGTGTTGTG
  GAATACTTAAGCAATGGAGGAGTAGCTGACAACCATAAAGACTTCAAGGAACTAAGGTACAATGAGTGTC
  TTATGAACTTCAGCTGCAATGGAAAGAATGGAAGCTCAGAAGGGAGAATCACACATGGCTTCCAACTTAA
  GAGCGCCTATGAAAATAACTTGATGCCTTACACCAATTACACCTTTGATTTCAAAGGCGTGATTGACTAC
  ATTTTCTATTCCAAGACTCATATGAACGTGCTTGGTGTCCTGGGGCCTTTAGATCCTCAATGGCTGGTTG
  AGAACAACATCACTGGGTGTCCACACCCTCACATCCCTTCAGACCACTTCTCACTGTTAACACAACTTGA
  ACTCCACCCTCCACTCCTGCCTCTTGTCAATGGTGTTCACTTGCCTAATCGGAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219766 representing NM_144571
  Red=Cloning site Green=Tags(s)
  
  MRLIGMPKEKYDPPDPRRIYTIMSAEEVANGKKSHWAELEISGRVRSLSTSLWSLTHLTALHLNDNYLSR
  IPPDIAKLHNLVYLDLSSNKLRSLPAELGNMVSLRELLLNNNLLRVLPYELGRLFQLQTLGLKGNPLSQD
  ILNLYQDPDGTRKLLNFMLDNLAVHPEQLPPRPWITLKERDQILPSASFTVMCYNVLCDKYATRQLYGYC
  PSWALNWEYRKKGIMEEIVNCDADIISLQEVETEQYFTLFLPALKERGYDGFFSPKSRAKIMSEQERKHV
  DGCAIFFKTEKFTLVQKHTVEFNQVAMANSDGSEAMLNRVMTKDNIGVAVVLEVHKELFGAGMKPIHAAD
  KQLLIVANAHMHWDPEYSDVKLIQTMMFVSEVKNILEKASSRPGSPTADPNSIPLVLCADLNSLPDSGVV
  EYLSNGGVADNHKDFKELRYNECLMNFSCNGKNGSSEGRITHGFQLKSAYENNLMPYTNYTFDFKGVIDY
  IFYSKTHMNVLGVLGPLDPQWLVENNITGCPHPHIPSDHFSLLTQLELHPPLLPLVNGVHLPNRR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144571
• ORF Size: 1665 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144571.2, NP_653172.2
• RefSeq Size: 8794
• RefSeq ORF: 1668
• Locus ID: 246175
• Domains: LRR, LRR_TYP, Exo_endo_phos, LRR_PS
• Protein Pathways: RNA degradation
• MW: 62.8 kDa