EYA1 (NM_000503) Human Tagged ORF Clone

CAT#: RC219782

EYA1 (Myc-DDK-tagged)-Human eyes absent homolog 1 (Drosophila) (EYA1), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY406816) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221561 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EYA1
• Synonyms: BOP; BOR; BOS1; OFC1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219782 representing NM_000503
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAAATGCAGGATCTAACCAGCCCGCATAGCCGTCTGAGTGGTAGTAGTGAATCCCCCAGTGGCCCCA
  AACTCGGTAACTCTCATATAAATAGTAATTCCATGACTCCCAATGGCACCGAAGTTAAAACAGAGCCAAT
  GAGCAGCAGTGAAACAGCTTCAACGACAGCCGACGGGTCTTTAAACAATTTCTCAGGTTCAGCAATTGGG
  AGCAGTAGTTTCAGCCCACGACCAACTCACCAGTTCTCTCCACCACAGATTTACCCTTCCAACAGACCAT
  ACCCACATATTCTCCCTACCCCTTCCTCACAAACTATGGCTGCATATGGGCAAACACAGTTTACCACAGG
  AATGCAACAAGCTACAGCCTATGCCACGTACCCACAGCCAGGACAGCCGTACGGCATTTCCTCATATGGT
  GCATTGTGGGCAGGCATCAAGACTGAAGGTGGATTGTCACAGTCTCAGTCACCTGGACAGACAGGATTTC
  TCAGCTATGGCACAAGCTTCAGTACCCCTCAACCTGGACAGGCACCATACAGCTACCAGATGCAAGGTAG
  CAGTTTTACAACATCATCAGGAATATATACAGGAAATAATTCACTCACAAATTCCTCTGGATTTAATAGT
  TCACAGCAGGACTATCCGTCTTATCCCAGTTTTGGCCAGGGTCAGTACGCACAGTATTATAACAGCTCAC
  CGTATCCAGCACATTATATGACCAGCAGCAACACCAGCCCAACGACACCATCCACCAATGCCACTTACCA
  GCTTCAAGAACCGCCATCTGGCATCACCAGCCAAGCAGTTACAGATCCCACAGCAGAGTACAGCACAATC
  CACAGCCCATCAACACCCATTAAAGATTCAGATTCTGATCGATTGCGTCGAGGTTCAGATGGGAAATCAC
  GTGGACGGGGCCGAAGAAACAATAATCCTTCACCTCCCCCAGATTCTGATCTTGAGAGAGTGTTCATCTG
  GGACTTGGATGAGACAATCATTGTTTTCCACTCCTTGCTTACTGGGTCCTACGCCAACAGATATGGGAGG
  GATCCACCCACTTCAGTTTCCCTTGGACTGCGAATGGAAGAAATGATTTTCAACTTGGCAGACACACATT
  TATTTTTTAATGACTTAGAAGAATGTGACCAAGTCCATATAGATGATGTTTCTTCAGATGATAACGGACA
  GGACCTAAGCACATATAACTTTGGAACAGATGGCTTTCCTGCTGCAGCAACCAGTGCTAACTTATGTTTG
  GCAACTGGTGTACGGGGCGGTGTGGACTGGATGAGAAAGTTGGCCTTCCGCTACAGACGGGTAAAAGAGA
  TCTACAACACCTACAAAAATAATGTTGGAGGTCTGCTTGGTCCAGCTAAGAGGGAAGCCTGGCTGCAGTT
  GAGGGCCGAAATTGAAGCCCTGACCGACTCCTGGTTGACACTGGCCCTGAAAGCACTCTCGCTCATTCAC
  TCCCGGACAAACTGTGTGAATATTTTAGTAACAACTACTCAGCTCATCCCAGCATTGGCGAAAGTCCTGC
  TGTATGGGTTAGGAATTGTATTTCCAATAGAAAATATTTACAGTGCAACTAAAATAGGAAAAGAAAGCTG
  TTTTGAGAGAATAATTCAAAGGTTTGGAAGAAAAGTGGTGTATGTTGTTATAGGAGATGGTGTAGAAGAA
  GAACAAGGAGCAAAAAAGCACGCGATGCCCTTCTGGAGGATCTCCAGCCACTCGGACCTCATGGCCCTGC
  ACCATGCCTTGGAACTGGAGTACCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219782 representing NM_000503
  Red=Cloning site Green=Tags(s)
  
  MEMQDLTSPHSRLSGSSESPSGPKLGNSHINSNSMTPNGTEVKTEPMSSSETASTTADGSLNNFSGSAIG
  SSSFSPRPTHQFSPPQIYPSNRPYPHILPTPSSQTMAAYGQTQFTTGMQQATAYATYPQPGQPYGISSYG
  ALWAGIKTEGGLSQSQSPGQTGFLSYGTSFSTPQPGQAPYSYQMQGSSFTTSSGIYTGNNSLTNSSGFNS
  SQQDYPSYPSFGQGQYAQYYNSSPYPAHYMTSSNTSPTTPSTNATYQLQEPPSGITSQAVTDPTAEYSTI
  HSPSTPIKDSDSDRLRRGSDGKSRGRGRRNNNPSPPPDSDLERVFIWDLDETIIVFHSLLTGSYANRYGR
  DPPTSVSLGLRMEEMIFNLADTHLFFNDLEECDQVHIDDVSSDDNGQDLSTYNFGTDGFPAAATSANLCL
  ATGVRGGVDWMRKLAFRYRRVKEIYNTYKNNVGGLLGPAKREAWLQLRAEIEALTDSWLTLALKALSLIH
  SRTNCVNILVTTTQLIPALAKVLLYGLGIVFPIENIYSATKIGKESCFERIIQRFGRKVVYVVIGDGVEE
  EQGAKKHAMPFWRISSHSDLMALHHALELEYL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000503
• ORF Size: 1776 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000503.2, NM_000503.3, NM_000503.4, NM_000503.5, NP_000494.2
• RefSeq Size: 4326 bp
• RefSeq ORF: 1779 bp
• Locus ID: 2138
• Cytogenetics: 8q13.3
• Domains: Hydrolase
• Protein Families: Druggable Genome, Phosphatase, Transcription Factors
• MW: 64.4 kDa
• Gene Summary: 'This gene encodes a member of the eyes absent (EYA) family of proteins. The encoded protein may play a role in the developing kidney, branchial arches, eye, and ear. Mutations of this gene have been associated with branchiootorenal dysplasia syndrome, branchiootic syndrome, and sporadic cases of congenital cataracts and ocular anterior segment anomalies. A similar protein in mice can act as a transcriptional activator. Alternatively spliced transcript variants have been identified for this gene. [provided by RefSeq, Dec 2013]'