SMPD4 (NM_017951) Human Tagged ORF Clone

CAT#: RC219824

SMPD4 (Myc-DDK-tagged)-Human sphingomyelin phosphodiesterase 4, neutral membrane (neutral sphingomyelinase-3) (SMPD4), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY413435) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC219824 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SMPD4
• Synonyms: NET13; NSMASE-3; NSMASE3; SKNY
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC219824 representing NM_017951
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGACTTTCGGCGCCGTGGCGGAATGGCGGCTTCCATCTCTGAGGCGAGCGACGCTATGGATCCCAC
  AGTGGTTTGCTAAGAAGGCCATTTTCAACTCTCCACTGGAGGCTGCTATGGCGTTCCCTCACCTGCAGCA
  GCCCAGCTTTCTACTGGCTAGCCTGAAAGCTGACTCTATAAATAAGCCCTTTGCACAGCAGTGCCAAGAC
  TTGGTTAAAGTCATTGAGGACTTTCCAGCAAAGGAGCTGCACACCATCTTCCCATGGCTGGTAGAAAGCA
  TTTTTGGCAGCCTAGATGGTGTCCTCGTTGGCTGGAACCTCCGCTGCTTACAGGGGCGCGTGAATCCTGT
  GGAGTACAGCATCGTGATGGAATTTCTCGACCCTGGTGGCCCAATGATGAAGTTGGTTTATAAGCTTCAA
  GCTGAAGACTATAAGTTCGACTTTCCTGTCTCCTACTTGCCTGGTCCTGTGAAGGCGTCCATCCAGGAGT
  GCATCCTCCCTGACAGTCCTCTGTACCACAACAAGGTCCAGTTCACCCCTACTGGGGGCCTTGGTCTGAA
  CTTGGCCCTGAATCCGTTCGAGTATTACATATTCTTCTTTGCCTTGAGCCTCATCACTCAGAAGCCACTT
  CCTGTGTCCCTCCACGTCCGTACTTCAGACTGTGCCTATTTCATCCTGGTGGACAGGTACCTGTCATGGT
  TCCTGCCCACCGAAGGCAGTGTGCCCCCACCACTCTCCTCCAGCCCAGGGGGGACCAGCCCCTCACCACC
  TCCCAGGACACCAGCCATACCCTTTGCTTCCTATGGCCTCCACCACACTAGCCTCCTAAAGCGACACATC
  TCTCATCAGACGTCTGTGAATGCAGACCCCGCCTCCCACGAGATCTGGAGGTCAGAAACTCTGCTCCAGG
  TTTTTGTTGAAATGTGGCTTCATCACTATTCCTTGGAGATGTATCAAAAAATGCAGTCCCCTCATGCCAA
  GCTGGAGGTTCTGCACTACCGACTCAGTGTCTCCAGCGCCCTCTACAGCCCCGCCCAACCCAGCCTCCAG
  GCCCTCCACGCCTACCAAGAGTCGTTCACGCCTACTGAGGAGCATGTGTTGGTGGTGCGCCTGCTGCTGA
  AGCACCTGCACGCCTTTGCCAACAGCCTGAAGCCAGAGCAGGCCTCACCCTCCGCCCACTCCCACGCCAC
  CAGCCCCCTGGAGGAGTTCAAACGGGCTGCTGTCCCGAGGTTCGTCCAGCAGAAACTCTACCTCTTCTTG
  CAGCATTGCTTTGGCCACTGGCCCCTGGACGCATCGTTCAGAGCTGTCCTGGAGATGTGGCTGAGCTACC
  TGCAGCCGTGGCGGTACGCGCCTGACAAGCAGGCTCCGGGCAGCGACTCCCAGCCCCGGTGTGTGTCGGA
  GAAATGGGCACCCTTTGTCCAGGAGAACCTGCTGATGTACACCAAGTTGTTTGTGGGCTTTCTGAACCGC
  GCGCTCCGCACAGACCTGGTCAGCCCCAAGCACGCGCTCATGGTGTTCCGAGTGGCCAAAGTCTTTGCCC
  AGCCCAACCTGGCTGAGATGATTCAGAAAGGTGAGCAGCTATTCCTGGAGCCAGAGCTGGTCATCCCCCA
  CCGCCAGCACCGACTCTTCACGGCCCCCACATTCACTGGGAGCTTCCTGTCACCCTGGCCACCAGCGGTC
  ACTGATGCCTCCTTCAAGGTGAAGAGCCACGTCTACAGCCTGGAGGGCCAGGACTGCAAGTACACCCCGA
  TGTTTGGGCCCGAGGCCCGCACCCTGGTCCTGCGCCTCGCTCAGCTCATCACACAGGCCAAACACACAGC
  CAAGTCCATCTCCGACCAGTGTGCGGAGAGCCCGGCTGGCCACTCCTTCCTCTCATGGCTGGGCTTTAGC
  TCCATGGACACCAATGGCTCCTACACAGCCAACGACCTGGACGAGATGGGGCAAGACAGTGTCCGGAAGA
  CAGATGAATACCTGGAGAAGGCCCTGGAGTACCTGCGCCAGATATTCCGGCTCAGCGAAGCGCAGCTCAG
  GCAGTTCACACTCGCCTTGGGCACCACCCAGGATGAGAATGGAAAAAAGCAACTCCCCGACTGCATCGTG
  GGTGAGGACGGACTCATCCTTACGCCCCTGGGGCGGTACCAGATCATCAATGGGCTGCGAAGGTTTGAAA
  TTGAGTACCAGGGGGACCCGGAGCTGCAGCCCATCCGGAGCTATGAGATCGCCAGCTTGGTCCGCACACT
  CTTTAGGCTGTCGTCTGCCATCAACCACAGATTTGCAGGACAGATGGCGGCTCTGTGTTCCCGGGATGAC
  TTCCTCGGCAGCTTCTGTCGCTACCACCTCACAGAACCTGGGCTGGCCAGCAGGCACCTGCTGAGCCCTG
  TGGGGCGGAGGCAGGTGGCCGGCCACACCCGCGGCCCCAGGCTCAGCCTGCGCTTCCTGGGCAGTTACCG
  GACGCTGGTCTCGCTGCTGCTGGCCTTCTTCGTGGCCTCTCTGTTCTGCGTCGGGCCCCTCCCATGCACG
  CTGCTGCTCACCCTGGGCTATGTCCTCTACGCCTCTGCCATGACACTGCTGACCGAGCGGGGGAAGCTGC
  ACCAGCCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC219824 representing NM_017951
  Red=Cloning site Green=Tags(s)
  
  MTTFGAVAEWRLPSLRRATLWIPQWFAKKAIFNSPLEAAMAFPHLQQPSFLLASLKADSINKPFAQQCQD
  LVKVIEDFPAKELHTIFPWLVESIFGSLDGVLVGWNLRCLQGRVNPVEYSIVMEFLDPGGPMMKLVYKLQ
  AEDYKFDFPVSYLPGPVKASIQECILPDSPLYHNKVQFTPTGGLGLNLALNPFEYYIFFFALSLITQKPL
  PVSLHVRTSDCAYFILVDRYLSWFLPTEGSVPPPLSSSPGGTSPSPPPRTPAIPFASYGLHHTSLLKRHI
  SHQTSVNADPASHEIWRSETLLQVFVEMWLHHYSLEMYQKMQSPHAKLEVLHYRLSVSSALYSPAQPSLQ
  ALHAYQESFTPTEEHVLVVRLLLKHLHAFANSLKPEQASPSAHSHATSPLEEFKRAAVPRFVQQKLYLFL
  QHCFGHWPLDASFRAVLEMWLSYLQPWRYAPDKQAPGSDSQPRCVSEKWAPFVQENLLMYTKLFVGFLNR
  ALRTDLVSPKHALMVFRVAKVFAQPNLAEMIQKGEQLFLEPELVIPHRQHRLFTAPTFTGSFLSPWPPAV
  TDASFKVKSHVYSLEGQDCKYTPMFGPEARTLVLRLAQLITQAKHTAKSISDQCAESPAGHSFLSWLGFS
  SMDTNGSYTANDLDEMGQDSVRKTDEYLEKALEYLRQIFRLSEAQLRQFTLALGTTQDENGKKQLPDCIV
  GEDGLILTPLGRYQIINGLRRFEIEYQGDPELQPIRSYEIASLVRTLFRLSSAINHRFAGQMAALCSRDD
  FLGSFCRYHLTEPGLASRHLLSPVGRRQVAGHTRGPRLSLRFLGSYRTLVSLLLAFFVASLFCVGPLPCT
  LLLTLGYVLYASAMTLLTERGKLHQP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_017951
• ORF Size: 2598 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_017951.2, NM_017951.3, NM_017951.4, NP_060421.2
• RefSeq Size: 4269
• RefSeq ORF: 2601
• Locus ID: 55627
• Protein Families: Transmembrane
• Protein Pathways: Metabolic pathways, Sphingolipid metabolism
• MW: 97.6 kDa
• Gene Summary: The protein encoded by this gene is a sphingomyelinase that catalyzes the hydrolysis of membrane sphingomyelin to form phosphorylcholine and ceramide. This gene is activated by DNA damage, cellular stress, and tumor necrosis factor, but it is downregulated by wild-type p53. The encoded protein localizes to the endoplasmic reticulum and Golgi network. [provided by RefSeq, Mar 2017]