OR8G1 (NM_001002905) Human Tagged ORF Clone

CAT#: RC220020

OR8G1 (Myc-DDK-tagged)-Human olfactory receptor, family 8, subfamily G, member 1 (OR8G1)

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OR8G1
• Synonyms: HSTPCR25; OR8G1P; TPCR25
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220020 representing NM_001002905
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCAGGAGAAAATAATTCCTCAGTGACTGAGTTCATTCTGGCTGGGCTCTCAGAACAGCCAGAGCTCC
  AGCTGCCCCTCTTCCTCCTGTTCTTAGGAATCTATGTGGTCACAGTGGTGGGCAACCTGGGCATGACCAC
  ACTGATTTGGCTCAGTTCTCACCTGCACACCCCTATGTACTATTTCCTCAGCAGTCTGTCCTTCATTGAC
  TTCTGCCATTCCACTGTCATTACCCCTAAGATGCTGGTGAACTTTGTGACAGAGAAGAACATCATCTCCT
  ACCCTGAATGCATGACTCAGCTCTACTTCTTCCTCGTTTTTGCTATTGCAGAGTGTCACATGTTGGCTGC
  AATGGCGTATGACCGTTACATGGCCATCTGTAGCCCCTTGCTGTACAGTGTCATCATATCCAATAAGGCT
  TGCTTTTCTCTGATTTTAGGGGTGTATATAATAGGCCTGGTTTGTGCATCAGTTCATACAGGCTGTATGT
  TTAGGGTTCAATTCTGCAAATTTGATTTGATTAACCATTATTTCTGTGATCTTCTTCCCCTCCTAAAGCT
  CTCTTGCTCTAGTATCTATGTCAACAAACTACTTATTCTATGTGTTGGTGCATTTAACATCCTTGTCCCC
  AGCCTGACCATCCTTTGCTCTTACATCTTTATTATTGCCAGCATCCTCCACATTCGCTCCACTGAGGGCA
  GGTCCAAAGCCTTCAGCACTTGTAGCTCCCACATGTTGGCGGTTGTAATCTTTTTTGGATCTGCAGCATT
  CATGTACTTGCAGCCATCTTCAATCAGCTCCATGGACCAGGGGAAAGTATCCTCTGTGTTTTATACTATT
  ATTGTGCCCATGTTGAACCCTCTGATTTATAGCCTGAGGAATAAAGATGTCCATGTTTCCCTGAAGAAAA
  TGCTACAGAGAAGAACATTATTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220020 representing NM_001002905
  Red=Cloning site Green=Tags(s)
  
  MSGENNSSVTEFILAGLSEQPELQLPLFLLFLGIYVVTVVGNLGMTTLIWLSSHLHTPMYYFLSSLSFID
  FCHSTVITPKMLVNFVTEKNIISYPECMTQLYFFLVFAIAECHMLAAMAYDRYMAICSPLLYSVIISNKA
  CFSLILGVYIIGLVCASVHTGCMFRVQFCKFDLINHYFCDLLPLLKLSCSSIYVNKLLILCVGAFNILVP
  SLTILCSYIFIIASILHIRSTEGRSKAFSTCSSHMLAVVIFFGSAAFMYLQPSSISSMDQGKVSSVFYTI
  IVPMLNPLIYSLRNKDVHVSLKKMLQRRTLL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001002905
• ORF Size: 933 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001002905.1, NP_001002905.1
• RefSeq Size: 936 bp
• RefSeq ORF: 936 bp
• Locus ID: 26494
• Cytogenetics: 11q24.2
• Protein Families: Transmembrane
• Protein Pathways: Olfactory transduction
• MW: 34.7 kDa
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. This family member represents a polymorphic pseudogene, whereby some individuals have a functional allele that encodes a full-length protein, while others have a non-functional allele due to the presence of an early stop codon and a 3' end deletion. [provided by RefSeq, Feb 2014]