CCDC138 (NM_144978) Human Tagged ORF Clone

CAT#: RC220044

CCDC138 (Myc-DDK-tagged)-Human coiled-coil domain containing 138 (CCDC138)

Product Images

Western blot validation of overexpression lysate (Cat# LY408119) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220044 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CCDC138 protein (Cat# TP320044). The protein was produced from HEK293T cells transfected with CCDC138 cDNA clone (Cat# RC220044) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCDC138
• Synonyms: FLJ32745
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220044 representing NM_144978
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGAGCCGAGGGTCGTCAAGCCACCGGGGCAGGATTTAGTAGTGGAGAGTCTCAAAAGCCGCTACGGAC
  TCGGGGGCAGCTGCCCCGACGAGTATGATTTTTCAAATTTTTATCAGTCTAAGTATAAGAGAAGAACTCT
  AACCTCCCCAGGTGATTTGGATATCTACTCTGGAGATAAAGTTGGTTCATCGTTAAAATATTCTGATGAA
  AGCAAGCATTGTAGAACACCATTGGGCAGCTTATTCAAGCACGTAAATGTGAATTGCCTAGATGATGAAC
  TGGATTCTTTCCATGATTTGAAGAAACAGGAAACAGAAGAAGAGTTAATTGAAAATGATTATAGAGTTAG
  TACCTCGAAAATAACCAAGCAGTCTTTTAAAGAAATAGAAAAAGTTGCCTTGCCAACTAATACGACCTCA
  TCGAGACCTCGGACTGAGTGTTGTAGTGATGCAGGTGACTCTCCTTTGAAACCTGTCAGCTGTCCAAAAT
  CTAAAGCATCAGACAAGCGGAGTTTACTTCCACATCAGATCAGTCAGATATATGACGAATTATTTCAGAT
  ACATCTGAAATTGCAGTGTGAAACTGCAGCACAACAGAAATTTGCTGAAGAACTTCAAAAGCGAGAACGT
  TTTTTACTTGAAAGAGAACAACTGCTTTTCAGACATGAAAATGCCTTGAGTAAAATTAAAGGTGTTGAAG
  AAGAGGTTCTTACAAGATTTCAAATTATAAAAGAGCAGCATGATGCAGAAGTTGAACACTTAACCGAAGT
  TCTTAAGGAAAAGAATAAAGAAACCAAGAGACTGAGGTCCTCTTTTGATGCATTGAAAGAATTGAATGAT
  ACCTTAAAAAAACAGTTAAATGAAGCAAGTGAAGAAAACAGGAAGATAGACATTCAGGCTAAAAGAGTTC
  AAGCACGTTTAGATAATTTACAGAGGAAGTACGAGTTTATGACAATACAGAGATTGAAAGGAAGTTCCCA
  TGCTGTTCATGAAATGAAAAGTTTAAAACAAGAAAAAGCACCAGTTTCAAAAACTTACAAGGTACCACTT
  AATGGGCAAGTTTATGAACTTTTAACTGTCTTCATGGACTGGATTTCGGATCATCATCTTAGCAAAGTGA
  AACATGAAGAATCTGGAATGGATGGTAAAAAACCACAACTCAAATTTGCTTCCCAGAGAAATGATATTCA
  GGAGAAGTGTGTAAAGCTTTTGCCTCTAATGACAGAGCAGCTACAGTGGATGCCATTTGTGAATATCAAA
  CTTCACGAGCCTTTTGTAAAATTTATATATTGGTCCCTAAGGCAGCTAGATGCTGGAGCACAGCACTCGA
  CTATGACATCAACATTGAGGAGATTGGGTGAAGACATTTTTAAAGGAGTGGTAACTAAAGGAATTCAGGA
  TAATTCTCCACAGCATTCTGTGGAGAATAAACCAAAGACAGCTGCTTTCTTTAAGAGCTCCAATTTGCCA
  TTGAGATTTTTATCAACCTTAATTGTTCTCAAAACAGTCACTCAAGCTGATTACCTGGCTCAGGCATTTG
  ATTCTCTTTGTTTGGACTTGAAGACAGAAGAAGGAAAAACCTTGTTTTTGGAGTATCAGGCTGTTCCAGT
  AATATTAAGTCATCTAAGAATATCCAGTAAAGGACTCCTGTCTAATGTTATTGATAGTTTGCTCCAGATG
  ACGGTGGAATCTAAATCCTTGCAGCCTTTCCTGGAAGCCTGTAGCAACTCTTTATTTTTTCGTACTTGCT
  CTGTGCTGCTTCGAGCCCCTAAGCTTGATCTTCAAATACTAGAAAAACTCAGTATTATTTTACAGAAACT
  TTCCAAAATCAAGAGTAATAAGAAGCTCTTTGAACTTTTTACGATTCATCTGATGCTTCAAGAAATACAA
  AGGACAACAAACCCAGAGCATGCATTTCTCTGTATTAATCTAAATTCAACTCTGTTCAATCTGGGTTTAA
  CAAAATGTAACTCCCTGGTCTCCAGTGCAAGCCCT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220044 representing NM_144978
  Red=Cloning site Green=Tags(s)
  
  MEPRVVKPPGQDLVVESLKSRYGLGGSCPDEYDFSNFYQSKYKRRTLTSPGDLDIYSGDKVGSSLKYSDE
  SKHCRTPLGSLFKHVNVNCLDDELDSFHDLKKQETEEELIENDYRVSTSKITKQSFKEIEKVALPTNTTS
  SRPRTECCSDAGDSPLKPVSCPKSKASDKRSLLPHQISQIYDELFQIHLKLQCETAAQQKFAEELQKRER
  FLLEREQLLFRHENALSKIKGVEEEVLTRFQIIKEQHDAEVEHLTEVLKEKNKETKRLRSSFDALKELND
  TLKKQLNEASEENRKIDIQAKRVQARLDNLQRKYEFMTIQRLKGSSHAVHEMKSLKQEKAPVSKTYKVPL
  NGQVYELLTVFMDWISDHHLSKVKHEESGMDGKKPQLKFASQRNDIQEKCVKLLPLMTEQLQWMPFVNIK
  LHEPFVKFIYWSLRQLDAGAQHSTMTSTLRRLGEDIFKGVVTKGIQDNSPQHSVENKPKTAAFFKSSNLP
  LRFLSTLIVLKTVTQADYLAQAFDSLCLDLKTEEGKTLFLEYQAVPVILSHLRISSKGLLSNVIDSLLQM
  TVESKSLQPFLEACSNSLFFRTCSVLLRAPKLDLQILEKLSIILQKLSKIKSNKKLFELFTIHLMLQEIQ
  RTTNPEHAFLCINLNSTLFNLGLTKCNSLVSSASP
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_144978
• ORF Size: 1995 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_144978.1, NM_144978.2, NP_659415.1
• RefSeq Size: 2196
• RefSeq ORF: 1998
• Locus ID: 165055
• MW: 76 kDa