PRODH (NM_016335) Human Tagged ORF Clone
CAT#: RC220096
PRODH (Myc-DDK-tagged)-Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1
"NM_016335" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | PRODH |
Synonyms | HSPOX2; PIG6; POX; PRODH1; PRODH2; TP53I6 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC220096 representing NM_016335
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTCTGAGGCGCGCCCTGCCCGCGCTGCGCCCCTGCATTCCCCGCTTCGTCCCGCTGTCCACGGCGC CGGCCTCCCGCGAGCAGCCCGCAGCGGGCCCAGCGGCCGTGCCAGGAGGTGGGTCGGCCACGGCAGTGCG GCCGCCGGTGCCCGCCGTGGACTTCGGCAACGCGCAGGAGGCGTACCGCAGCCGGCGAACCTGGGAGCTG GCGCGGAGCCTGCTGGTGCTGCGCTTGTGCGCCTGGCCCGCGCTGCTGGCGCGCCACGAGCAGCTGCTGT ATGTTTCCAGGAAACTTCTAGGACAGAGGCTATTCAACAAGCTCATGAAGATGACCTTCTATGGGCATTT TGTAGCCGGGGAGGACCAGGAGTCCATCCAGCCCCTGCTTCGGCACTACAGGGCCTTCGGTGTCAGCGCC ATCCTGGACTATGGAGTGGAGGAGGACCTGAGCCCCGAGGAGGCAGAGCACAAGGAGATGGAGTCCTGCA CCTCAGCTGCGGAGAGGGATGGCAGTGGCACGAATAAGCGGGACAAGCAATACCAGGCCCACCGGGCCTT CGGGGACCGCAGGAATGGTGTCATCAGTGCCCGCACCTACTTCTACGCCAATGAGGCCAAGTGCGACAGC CACATGGAGACATTCTTGCGCTGCATCGAAGCCTCAGGTAGAGTCAGCGATGACGGCTTCATAGCCATTA AGCTCACAGCACTGGGGAGACCCCAGTTTCTGCTGCAGTTCTCAGAGGTGCTGGCCAAGTGGAGGTGCTT CTTTCACCAAATGGCTGTGGAGCAAGGGCAGGCGGGCCTGGCTGCCATGGACACCAAGCTGGAGGTGGCG GTGCTGCAGGAAAGTGTCGCAAAGTTGGGCATCGCATCCAGGGCTGAGATTGAGGACTGGTTCACGGCAG AGACCCTGGGAGTGTCTGGCACCATGGACCTGCTGGACTGGAGCAGCCTCATCGACAGCAGGACCAAGCT GTCCAAGCACTTGGTAGTCCCCAACGCACAGACAGGACAGCTGGAGCCCCTGCTGTCCCGGTTCACTGAG GAGGAGGAGCTACAGATGACCAGGATGCTACAGCGGATGGATGTCCTGGCCAAGAAAGCCACAGAGATGG GCGTGCGGCTGATGGTGGATGCCGAGCAGACCTACTTCCAGCCGGCCATCAGCCGCCTGACGCTGGAGAT GCAGCGGAAGTTCAATGTGGAGAAGCCGCTCATCTTCAACACATACCAGTGCTACCTCAAGGATGCCTAT GACAATGTGACCCTGGACGTGGAGCTGGCTCGCCGTGAGGGCTGGTGTTTTGGGGCCAAGCTGGTGCGGG GCGCATACCTGGCCCAGGAGCGAGCCCGTGCGGCAGAGATCGGCTATGAGGACCCCATCAACCCCACGTA CGAGGCCACCAACGCCATGTACCACAGGTGCCTGGACTACGTGTTGGAGGAGCTGAAGCACAACGCCAAG GCCAAGGTGATGGTGGCCTCCCACAATGAGGACACAGTGCGCTTTGCACTGCGCAGGATGGAGGAGCTGG GCCTGCATCCTGCTGACCACCGGGTGTACTTTGGACAGCTGCTAGGCATGTGTGACCAGATCAGCTTCCC GCTGGGCCAGGCTGGCTACCCCGTGTACAAGTACGTGCCCTATGGCCCCGTGATGGAGGTGCTGCCCTAC TTGTCCCGCCGTGCCCTGGAGAACAGCAGCCTCATGAAGGGCACCCATCGGGAGCGGCAGCTGCTGTGGC TGGAGCTCTTGAGGCGGCTCCGAACTGGCAACCTCTTCCATCGCCCTGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220096 representing NM_016335
Red=Cloning site Green=Tags(s) MALRRALPALRPCIPRFVPLSTAPASREQPAAGPAAVPGGGSATAVRPPVPAVDFGNAQEAYRSRRTWEL ARSLLVLRLCAWPALLARHEQLLYVSRKLLGQRLFNKLMKMTFYGHFVAGEDQESIQPLLRHYRAFGVSA ILDYGVEEDLSPEEAEHKEMESCTSAAERDGSGTNKRDKQYQAHRAFGDRRNGVISARTYFYANEAKCDS HMETFLRCIEASGRVSDDGFIAIKLTALGRPQFLLQFSEVLAKWRCFFHQMAVEQGQAGLAAMDTKLEVA VLQESVAKLGIASRAEIEDWFTAETLGVSGTMDLLDWSSLIDSRTKLSKHLVVPNAQTGQLEPLLSRFTE EEELQMTRMLQRMDVLAKKATEMGVRLMVDAEQTYFQPAISRLTLEMQRKFNVEKPLIFNTYQCYLKDAY DNVTLDVELARREGWCFGAKLVRGAYLAQERARAAEIGYEDPINPTYEATNAMYHRCLDYVLEELKHNAK AKVMVASHNEDTVRFALRRMEELGLHPADHRVYFGQLLGMCDQISFPLGQAGYPVYKYVPYGPVMEVLPY LSRRALENSSLMKGTHRERQLLWLELLRRLRTGNLFHRPA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_016335 |
ORF Size | 1800 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_016335.1, NM_016335.2, NM_016335.3, NM_016335.4, NP_057419.2, NP_057419.4 |
RefSeq Size | 2400 bp |
RefSeq ORF | 1803 bp |
Locus ID | 5625 |
Cytogenetics | 22q11.21 |
Protein Families | Druggable Genome |
Protein Pathways | Arginine and proline metabolism, Metabolic pathways |
MW | 67.8 kDa |
Gene Summary | 'This gene encodes a mitochondrial protein that catalyzes the first step in proline degradation. Mutations in this gene are associated with hyperprolinemia type 1 and susceptibility to schizophrenia 4 (SCZD4). This gene is located on chromosome 22q11.21, a region which has also been associated with the contiguous gene deletion syndromes, DiGeorge and CATCH22. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Aug 2010]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC304405 | PRODH (untagged)-Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 1,020.00 |
|
RG220096 | PRODH (GFP-tagged) - Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1 |
USD 530.00 |
|
RC220096L1 | Lenti ORF clone of Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
USD 680.00 |
|
RC220096L2 | Lenti ORF clone of Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
USD 680.00 |
|
RC220096L3 | Lenti ORF clone of Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
USD 840.00 |
|
RC220096L4 | Lenti ORF clone of Human proline dehydrogenase (oxidase) 1 (PRODH), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
USD 680.00 |
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