P2Y10 (P2RY10) (NM_198333) Human Tagged ORF Clone

CAT#: RC220149

P2RY10 (Myc-DDK-tagged)-Human purinergic receptor P2Y, G-protein coupled, 10 (P2RY10), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY405022) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220149 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: P2RY10
• Synonyms: LYPSR2; P2Y10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220149 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCTAACCTTGACAAATACACTGAAACATTCAAGATGGGTAGCAACAGTACCAGCACTGCTGAGATTT
  ACTGTAATGTCACTAATGTGAAATTTCAATACTCCCTCTATGCAACCACCTATATCCTCATATTCATTCC
  TGGTCTTCTGGCTAACAGTGCAGCCTTGTGGGTTCTGTGCCGCTTCATCAGCAAGAAAAATAAAGCCATC
  ATTTTCATGATCAACCTCTCTGTGGCTGACCTTGCTCATGTATTATCTTTACCCCTCCGGATTTACTATT
  ACATCAGCCACCACTGGCCTTTCCAGAGAGCCCTTTGCCTGCTCTGCTTCTACCTGAAGTATCTCAACAT
  GTATGCCAGCATTTGTTTCCTGACGTGCATCAGTCTTCAAAGGTGCTTTTTTCTCCTCAAGCCCTTCAGG
  GCCAGAGACTGGAAGCGTAGGTACGATGTGGGCATCAGTGCTGCCATCTGGATCGTTGTGGGGACTGCCT
  GTTTGCCATTTCCCATCCTGAGAAGCACAGACTTAAACAACAACAAGTCCTGCTTTGCTGATCTTGGATA
  CAAGCAAATGAATGCAGTTGCGTTGGTCGGGATGATTACAGTTGCTGAGCTTGCAGGATTTGTGATCCCA
  GTGATCATCATCGCATGGTGTACCTGGAAAACTACTATATCCTTGAGACAGCCACCAATGGCTTTCCAAG
  GGATCAGTGAGAGGCAGAAAGCACTGCGGATGGTGTTCATGTGTGCTGCAGTCTTCTTCATCTGCTTCAC
  TCCCTATCATATTAACTTTATTTTTTACACCATGGTAAAGGAAACCATCATTAGCAGTTGTCCCGTTGTC
  CGAATCGCACTGTATTTCCACCCTTTTTGCCTGTGCCTTGCAAGTCTCTGCTGCCTTTTGGATCCAATTC
  TTTATTACTTTATGGCTTCAGAGTTTCGTGACCAACTATCCCGCCATGGCAGTTCTGTGACCCGCTCCCG
  CCTCATGAGCAAGGAGAGTGGTTCATCAATGATTGGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220149 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MANLDKYTETFKMGSNSTSTAEIYCNVTNVKFQYSLYATTYILIFIPGLLANSAALWVLCRFISKKNKAI
  IFMINLSVADLAHVLSLPLRIYYYISHHWPFQRALCLLCFYLKYLNMYASICFLTCISLQRCFFLLKPFR
  ARDWKRRYDVGISAAIWIVVGTACLPFPILRSTDLNNNKSCFADLGYKQMNAVALVGMITVAELAGFVIP
  VIIIAWCTWKTTISLRQPPMAFQGISERQKALRMVFMCAAVFFICFTPYHINFIFYTMVKETIISSCPVV
  RIALYFHPFCLCLASLCCLLDPILYYFMASEFRDQLSRHGSSVTRSRLMSKESGSSMIG
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_198333
• ORF Size: 1017 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_198333.1, NM_198333.2, NP_938147.1
• RefSeq Size: 1679 bp
• RefSeq ORF: 1020 bp
• Locus ID: 27334
• Protein Families: Druggable Genome, GPCR, Transmembrane
• Protein Pathways: Neuroactive ligand-receptor interaction
• MW: 38.8 kDa
• Gene Summary: The protein encoded by this gene belongs to the family of G-protein coupled receptors that are preferentially activated by adenosine and uridine nucleotides. There is a pseudogene for this gene nearby on chromosome X. Multiple alternatively spliced transcripts have been observed. [provided by RefSeq, Apr 2016]