Huntingtin Associated Protein 1 (HAP1) (NM_001079870) Human Tagged ORF Clone

CAT#: RC220305

HAP1 (Myc-DDK-tagged)-Human huntingtin-associated protein 1 (HAP1), transcript variant 3

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: HAP1
• Synonyms: HAP2; hHLP1; HIP5; HLP
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220305 representing NM_001079870
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCGCCCGAAGAGGTTGGGCCGGTGCTGCGCGGGGAGCCGGCTCGGACCCGGGGACCCAGCAGCACTCA
  CCTGTGCACCTTCGCCCTCAGCCAGTCCCGCTCCGGAGCCCTCTGCGCAGCCGCAGGCACGGGGCACTGG
  ACAGAGAGTAGGATCCCGAGCCACCTCTGGATCCCAGTTCCTCTCGGAAGCCCGCACCGGAGCTCGCCCG
  GCCTCGGAGGCTGGAGCCAAGGCAGGAGCCCGGCGCCCGTCCGCATTCTCGGCCATCCAAGGGGATGTCC
  GGTCTATGCCCGACAATTCGGACGCGCCGTGGACCCGCTTCGTATTCCAAGGGCCGTTTGGTTCCCGGGC
  CACTGGCCGGGGGACTGGAAAGGCAGCGGGCATCTGGAAGACGCCAGCCGCCTACGTTGGCCGGCGACCC
  GGGGTGTCCGGCCCTGAGCGCGCCGCCTTTATTCGGGAGCTGGAGGAAGCACTGTGTCCTAACCTACCTC
  CGCCAGTCAAAAAGATCACCCAGGAAGACGTCAAAGTGATGTTATATTTGCTGGAGGAGGTGTGTACTGC
  CTTCCTGATCCAGCTTCTCCCACCTGTCTGGGAGAGCGTTACCTATGGGATGGTCCTGCAGAGAGAGAGG
  GACCTGAACACTGCAGCTCGCATCGGCCAGTCCCTGGTGAAACAGAACAGTGTTTTGATGGAGGAGAACA
  GCAAGCTGGAAGCCCTGCTGGGCTCAGCCAAGGAGGAGATTTTATACCTCAGACACCAGGTGAACTTGCG
  GGATGAGCTCCTCCAGCTCTACTCAGATTCTGATGAGGAGGATGAGGATGAAGAAGAGGAGGAGGAAGAA
  AAGGAGGCAGAAGAGGAACAGGAAGAAGAAGAAGCAGAGGAAGACCTGCAGTGTGCTCATCCCTGTGATG
  CCCCTAAGCTGATTTCGCAGGAGGCATTGCTGCACCAGCACCACTGCCCACAGCTGGAAGCCTTGCAGGA
  GAAGCTGAGGCTGCTGGAGGAGGAGAATCATCAGCTGAGAGAAGAGGCCTCTCAACTCGACACTCTTGAG
  GATGAGGAACAGATGCTCATTCTGGAGTGTGTGGAGCAGTTTTCGGAGGCCAGCCAACAGATGGCTGAGC
  TGTCGGAGGTGCTGGTGCTCAGGCTGGAAAACTATGAACGGCAGCAGCAGGAGGTCGCTCGGCTGCAGGC
  CCAGGTGCTGAAGCTGCAGCAGCGCTGCCGGATGGAGACTCTTCCTGGTTTCCAGGAGACGCTGGCTGAG
  GAGCTCAGAACGTCTCTAAGGAGGATGATCTCAGACCCTGTGTATTTTATGGAGAGGAATTATGAGATGC
  CCAGAGGGGACACATCCAGCCTAAGGTATGATTTTCGCTACAGTGAGGATCGAGAGCAGGTGCGGGGGTT
  TGAGGCTGAGGAAGGGTTGATGCTGGCAGCGGATATCATGCGGGGGGAAGATTTCACGCCTGCGGAGGAG
  TTCGTGCCCCAGGAGGAGCTGGGGGCTGCCAAGAAGGTGCCGGCTGAGGAAGGGGTGATGGAAGAGGCAG
  AGCTGGTGTCAGAGGAGACCGAGGGCTGGGAGGAGGTGGAACTGGAGCTGGATGAGGCAACGCGGATGAA
  CGTGGTGACATCAGCCCTGGAGGCCAGCGGCTTGGGCCCTTCACACCTGGACATGAATTATGTCCTCCAG
  CAGCTGGCCAACTGGCAAGATGCCCATTACAGGCGGCAGCTGAGGTGGAAGATGCTCCAGAAAGGTGAGT
  GCCCCCACGGGGCCCTCCCTGCCGCCAGCCGGACAAGCTGCAGATCGTCGTGCCGA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220305 representing NM_001079870
  Red=Cloning site Green=Tags(s)
  
  MRPKRLGRCCAGSRLGPGDPAALTCAPSPSASPAPEPSAQPQARGTGQRVGSRATSGSQFLSEARTGARP
  ASEAGAKAGARRPSAFSAIQGDVRSMPDNSDAPWTRFVFQGPFGSRATGRGTGKAAGIWKTPAAYVGRRP
  GVSGPERAAFIRELEEALCPNLPPPVKKITQEDVKVMLYLLEEVCTAFLIQLLPPVWESVTYGMVLQRER
  DLNTAARIGQSLVKQNSVLMEENSKLEALLGSAKEEILYLRHQVNLRDELLQLYSDSDEEDEDEEEEEEE
  KEAEEEQEEEEAEEDLQCAHPCDAPKLISQEALLHQHHCPQLEALQEKLRLLEEENHQLREEASQLDTLE
  DEEQMLILECVEQFSEASQQMAELSEVLVLRLENYERQQQEVARLQAQVLKLQQRCRMETLPGFQETLAE
  ELRTSLRRMISDPVYFMERNYEMPRGDTSSLRYDFRYSEDREQVRGFEAEEGLMLAADIMRGEDFTPAEE
  FVPQEELGAAKKVPAEEGVMEEAELVSEETEGWEEVELELDEATRMNVVTSALEASGLGPSHLDMNYVLQ
  QLANWQDAHYRRQLRWKMLQKGECPHGALPAASRTSCRSSCR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001079870
• ORF Size: 1806 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_001079870.1, NP_001073339.1
• RefSeq Size: 3893
• RefSeq ORF: 1809
• Locus ID: 9001
• Protein Pathways: Huntington's disease
• MW: 67.4 kDa
• Gene Summary: Huntington's disease (HD), a neurodegenerative disorder characterized by loss of striatal neurons, is caused by an expansion of a polyglutamine tract in the HD protein huntingtin. This gene encodes a protein that interacts with huntingtin, with two cytoskeletal proteins (dynactin and pericentriolar autoantigen protein 1), and with a hepatocyte growth factor-regulated tyrosine kinase substrate. The interactions with cytoskeletal proteins and a kinase substrate suggest a role for this protein in vesicular trafficking or organelle transport. Several alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008]