CRNKL1 (NM_016652) Human Tagged ORF Clone

CAT#: RC220334

CRNKL1 (Myc-DDK-tagged)-Human crooked neck pre-mRNA splicing factor-like 1 (Drosophila) (CRNKL1)

Product Images

Western blot validation of overexpression lysate (Cat# LY413851) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220334 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CRNKL1
• Synonyms: CLF; Clf1; CRN; HCRN; MSTP021; SYF3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220334 representing NM_016652
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACGGCAACAGTGGAGAACCTAACGTTCCAGAAGGACACTTTGGGAAATGCTGTTGACAAAAACACAT
  CCAGATTGGAGCTCCGTTCTTACTCCCTTGCAGGGCGCCACGGCTCGACAGAGCCACTCGTCCTCGCCTG
  GTCATCCCAGTTCCGGAGGCTGACCTGGGGATGCGCCCTGGACGCCCTGCACCGCAGTCCTTGCGTCGCT
  GCTAGTCAGCACGGCGTTACCCACCTCATCCGCAGCTCCAGGACGCCGCACTCCACGCGCTGCCGCAAGG
  AGGACGCGCAGCCTGGTCACCATGGAAACGGTGCCGCCTCCGTCACGGCTCAAGCGAGAGGTCAAAGGTC
  AGTCCTGCAAGTTCCGCTTCCGGTTCCGAGATCCTGTTTGTTTTCTGAAAGCTTTGTGGTTTCGGTGAGC
  TCTCAGAGCCGATTTCTAGCGTCCGTGCCGGGGACAGGTGTCCAGAGGTCGACTGCTGCAGACATGGCGG
  CCTCCACCGCGGCCGGGAAGCAGCGGATTCCCAAAGTGGCCAAGGTGAAAAACAAAGCCCCGGCTGAGGT
  ACAGATAACTGCTGAACAACTCTTAAGAGAGGCTAAAGAAAGAGAACTTGAGCTTCTTCCACCTCCACCT
  CAACAGAAGATCACAGATGAAGAAGAATTAAATGATTATAAACTAAGGAAAAGGAAGACTTTTGAAGATA
  ATATAAGAAAAAACAGGACTGTGATTAGTAACTGGATAAAATACGCACAATGGGAAGAAAGCCTAAAGGA
  GATTCAAAGGGCTCGATCCATATACGAGCGTGCTTTAGATGTAGACTACCGAAATATTACACTCTGGCTG
  AAATACGCAGAAATGGAAATGAAGAATCGCCAAGTCAACCATGCTCGAAATATCTGGGACCGGGCCATAA
  CAACGCTGCCTCGAGTTAATCAGTTCTGGTACAAGTACACGTACATGGAGGAAATGTTGGGAAACGTTGC
  CGGTGCCCGGCAGGTGTTTGAGCGCTGGATGGAGTGGCAGCCTGAGGAGCAAGCCTGGCACTCCTACATC
  AACTTTGAGCTGAGATACAAAGAGGTGGATCGGGCCCGCACCATTTATGAGCGATTTGTCCTCGTGCACC
  CTGATGTTAAGAACTGGATCAAGTATGCCCGCTTTGAAGAAAAACATGCTTATTTTGCCCATGCACGGAA
  AGTGTATGAGAGAGCTGTGGAATTCTTTGGAGATGAACATATGGATGAGCACCTTTATGTTGCCTTTGCC
  AAGTTTGAAGAAAATCAGAAAGAGTTTGAAAGGGTACGAGTGATTTACAAGTATGCCCTGGACAGAATTT
  CAAAACAAGATGCCCAAGAACTCTTTAAAAATTATACCATCTTTGAGAAGAAGTTTGGTGATAGGCGGGG
  TATTGAAGATATCATTGTGAGCAAACGGAGATTCCAGTACGAAGAAGAAGTGAAGGCGAATCCACACAAT
  TATGATGCATGGTTTGATTACTTGCGCTTGGTAGAAAGTGACGCAGAAGCTGAAGCCGTGAGAGAAGTCT
  ATGAAAGGGCCATTGCCAATGTCCCACCCATTCAGGAGAAGAGGCACTGGAAGCGCTACATTTATCTTTG
  GATCAACTATGCACTCTATGAAGAATTGGAGGCAAAGGATCCTGAGAGGACAAGACAGGTGTATCAAGCC
  TCTTTGGAACTAATTCCTCACAAAAAGTTCACATTTGCCAAAATGTGGATACTGTATGCACAGTTTGAAA
  TACGACAGAAGAATCTGTCATTAGCCAGAAGAGCATTGGGAACTTCCATAGGCAAATGTCCAAAGAACAA
  ATTATTTAAAGTTTACATAGAATTGGAGCTACAGCTTCGAGAATTTGACAGATGCCGGAAGCTTTATGAA
  AAGTTCCTGGAATTTGGACCTGAAAATTGTACCTCATGGATTAAATTCGCTGAATTAGAGACAATCCTTG
  GTGATATTGACAGAGCACGGGCAATCTATGAATTAGCCATCAGTCAGCCACGTTTAGACATGCCAGAGGT
  GCTTTGGAAATCATATATTGATTTTGAAATTGAGCAGGAAGAAACAGAAAGAACACGAAACCTTTACCGG
  CGGTTGCTTCAACGGACGCAGCATGTCAAGGTATGGATCAGCTTTGCTCAGTTTGAGTTGTCTTCAGGAA
  AAGAAGGAAGTTTGACTAAATGCAGACAAATTTATGAAGAAGCTAACAAAACCATGCGAAACTGTGAAGA
  AAAGGAAGAGAGACTTATGCTGCTGGAATCTTGGCGAAGTTTTGAAGAAGAATTTGGAACAGCTTCAGAT
  AAGGAGAGAGTAGACAAACTCATGCCAGAGAAAGTCAAGAAGAGAAGAAAGGTCCAGACTGATGATGGGT
  CTGATGCAGGCTGGGAAGAATACTTTGATTACATCTTTCCAGAAGATGCTGCCAACCAACCTAACCTCAA
  ACTCCTGGCCATGGCCAAACTGTGGAAGAAACAGCAGCAGGAAAAGGAGGATGCTGAGCACCATCCAGAT
  GAGGACGTCGATGAGAGTGAATCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220334 representing NM_016652
  Red=Cloning site Green=Tags(s)
  
  MTATVENLTFQKDTLGNAVDKNTSRLELRSYSLAGRHGSTEPLVLAWSSQFRRLTWGCALDALHRSPCVA
  ASQHGVTHLIRSSRTPHSTRCRKEDAQPGHHGNGAASVTAQARGQRSVLQVPLPVPRSCLFSESFVVSVS
  SQSRFLASVPGTGVQRSTAADMAASTAAGKQRIPKVAKVKNKAPAEVQITAEQLLREAKERELELLPPPP
  QQKITDEEELNDYKLRKRKTFEDNIRKNRTVISNWIKYAQWEESLKEIQRARSIYERALDVDYRNITLWL
  KYAEMEMKNRQVNHARNIWDRAITTLPRVNQFWYKYTYMEEMLGNVAGARQVFERWMEWQPEEQAWHSYI
  NFELRYKEVDRARTIYERFVLVHPDVKNWIKYARFEEKHAYFAHARKVYERAVEFFGDEHMDEHLYVAFA
  KFEENQKEFERVRVIYKYALDRISKQDAQELFKNYTIFEKKFGDRRGIEDIIVSKRRFQYEEEVKANPHN
  YDAWFDYLRLVESDAEAEAVREVYERAIANVPPIQEKRHWKRYIYLWINYALYEELEAKDPERTRQVYQA
  SLELIPHKKFTFAKMWILYAQFEIRQKNLSLARRALGTSIGKCPKNKLFKVYIELELQLREFDRCRKLYE
  KFLEFGPENCTSWIKFAELETILGDIDRARAIYELAISQPRLDMPEVLWKSYIDFEIEQEETERTRNLYR
  RLLQRTQHVKVWISFAQFELSSGKEGSLTKCRQIYEEANKTMRNCEEKEERLMLLESWRSFEEEFGTASD
  KERVDKLMPEKVKKRRKVQTDDGSDAGWEEYFDYIFPEDAANQPNLKLLAMAKLWKKQQQEKEDAEHHPD
  EDVDESES
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_016652
• ORF Size: 2544 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_016652.3, NM_016652.4, NM_016652.5, NP_057736.3, NP_057736.4
• RefSeq Size: 4406
• RefSeq ORF: 2547
• Locus ID: 51340
• Protein Pathways: Spliceosome
• MW: 100.3 kDa
• Gene Summary: The crooked neck (crn) gene of Drosophila is essential for embryogenesis and is thought to be involved in cell cycle progression and pre-mRNA splicing. A protein encoded by this human locus has been found to localize to pre-mRNA splicing complexes in the nucleus and is necessary for pre-mRNA splicing. Alternatively spliced transcript variants have been described. [provided by RefSeq, Jul 2013]