TTYH1 (NM_020659) Human Tagged ORF Clone

CAT#: RC220380

TTYH1 (Myc-DDK-tagged)-Human tweety homolog 1 (Drosophila) (TTYH1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY412397) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220380 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TTYH1
• Synonyms: 4930459B04Rik; 6330408P11Rik; OTTMUSP00000024583; tty; tweety 1; tweety homolog 1 (Drosophila)
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220380 representing NM_020659
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGGGCGCCCCCGGGCTACCGGCCCTCAGCTTGGGTGCATCTCCTCCACCAGCTGCCCCGCGCCGACT
  TCCAGCTCCGCCCGGTGCCCAGCGTTTTCGCGCCCCAAGAGCAGGAATACCAGCAGGCCTTGTTGCTGGT
  GGCGGCCTTGGCGGGCCTGGGCTTGGGCCTGAGCCTCATTTTCATCGCTGTCTACCTCATCCGCTTCTGC
  TGCTGCCGGCCCCCCGAGCCCCCCGGGTCCAAGATCCCCTCGCCCGGGGGAGGCTGCGTCACCTGGAGCT
  GCATTGTCGCCCTTCTCGCCGGCTGCACTGGCATTGGCATCGGTTTCTATGGCAACAGTGAGACCAGTGA
  TGGGGTGTCCCAGCTCAGCTCTGCGCTGCTGCACGCCAACCACACACTCAGCACCATTGACCACCTGGTG
  TTGGAGACGGTGGAGAGGCTGGGCGAGGCGGTGAGGACAGAGCTGACCACCCTGGAGGAGGTGCTCGAGC
  CGCGCACGGAGCTGGTGGCTGCCGCCCGAGGGGCTCGACGGCAGGCGGAGGCTGCGGCCCAGCAGCTGCA
  GGGGCTGGCCTTCTGGCAGGGAGTGCCCCTGAGCCCCCTGCAGGTGGCTGAAAATGTGTCCTTTGTGGAG
  GAGTACAGGTGGCTGGCCTACGTCCTCCTGCTGCTCCTGGAGCTGCTGGTCTGCCTCTTCACCCTCCTGG
  GCCTGGCGAAGCAGAGCAAGTGGCTGGTGATCGTGATGACAGTCATGAGTCTCCTGGTTCTCGTCCTGAG
  CTGGGGCTCCATGGGCCTGGAGGCAGCCACGGCCGTGGGCCTCAGTGACTTCTGCTCCAATCCAGACCCT
  TATGTTCTGAACCTGACCCAGGAGGAGACAGGGCTCAGCTCAGACATCCTGAGCTATTATCTCCTCTGCA
  ACCGGGCCGTCTCCAACCCCTTCCAACAGAGGCTGACTCTGTCCCAGCGAGCTCTGGCCAACATCCACTC
  CCAGCTGCTGGGCCTGGAGCGAGAAGCTGTGCCTCAGTTCCCTTCAGCGCAGAAGCCTCTGCTGTCCTTG
  GAGGAGACTCTGAATGTGACAGAAGGAAATTTCCACCAGTTGGTGGCACTGCTACACTGCCGCAGCCTGC
  ACAAGGACTATGGTGCAGCCCTGCGGGGCCTGTGCGAAGACGCCCTGGAAGGCCTGCTCTTCCTGCTACT
  CTTCTCCCTGCTGTCTGCAGGAGCGCTGGCCACTGCCCTCTGCAGCCTGCCCCGAGCCTGGGCCCTCTTC
  CCACCCAGTGACGACTACGATGACACAGACGATGACGACCCTTTCAACCCTCAGGAATCCAAGCGCTTTG
  TGCAGTGGCAGTCGTCTATC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220380 representing NM_020659
  Red=Cloning site Green=Tags(s)
  
  MGAPPGYRPSAWVHLLHQLPRADFQLRPVPSVFAPQEQEYQQALLLVAALAGLGLGLSLIFIAVYLIRFC
  CCRPPEPPGSKIPSPGGGCVTWSCIVALLAGCTGIGIGFYGNSETSDGVSQLSSALLHANHTLSTIDHLV
  LETVERLGEAVRTELTTLEEVLEPRTELVAAARGARRQAEAAAQQLQGLAFWQGVPLSPLQVAENVSFVE
  EYRWLAYVLLLLLELLVCLFTLLGLAKQSKWLVIVMTVMSLLVLVLSWGSMGLEAATAVGLSDFCSNPDP
  YVLNLTQEETGLSSDILSYYLLCNRAVSNPFQQRLTLSQRALANIHSQLLGLEREAVPQFPSAQKPLLSL
  EETLNVTEGNFHQLVALLHCRSLHKDYGAALRGLCEDALEGLLFLLLFSLLSAGALATALCSLPRAWALF
  PPSDDYDDTDDDDPFNPQESKRFVQWQSSI
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_020659
• ORF Size: 1350 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_020659.1, NM_020659.2, NM_020659.3, NP_065710.1
• RefSeq Size: 2045
• RefSeq ORF: 1353
• Locus ID: 57348
• Protein Families: Ion Channels: Other, Transmembrane
• MW: 48.9 kDa
• Gene Summary: This gene encodes a member of the tweety family of proteins. Members of this family function as chloride anion channels. The encoded protein functions as a calcium(2+)-independent, volume-sensitive large conductance chloride(-) channel. Three transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq, Jan 2011]