DFNB31 (WHRN) (NM_015404) Human Tagged ORF Clone

CAT#: RC220471

DFNB31 (Myc-DDK-tagged)-Human deafness, autosomal recessive 31 (DFNB31), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY414571) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220471 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: WHRN
• Synonyms: CIP98; DFNB31; PDZD7B; USH2D; WI
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220471 representing NM_015404
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACGCGCCGCTGGACGGCCTGTCGGTGAGCTCGTCCTCCACCGGCTCGCTGGGCTCGGCGGCCGGGG
  CGGGCGGCGGCGGGGGCGCGGGGCTGCGGTTACTGTCTGCCAACGTGCGCCAGCTGCACCAAGCGCTGAC
  CGCGCTGCTGAGCGAGGCGGAGCGGGAGCAGTTCACCCACTGCCTGAACGCTTACCACGCGCGCCGCAAC
  GTCTTCGACCTGGTGCGCACCCTGCGCGTGCTGCTGGACAGTCCGGTCAAGCGGCGCCTGCTGCCCATGC
  TTCGTCTGGTCATCCCGCGCTCCGACCAGCTGCTCTTCGACCAATACACGGCCGAGGGCCTCTACCTGCC
  CGCCACCACCCCCTACAGGCAGCCCGCCTGGGGCGGCCCCGACAGCGCGGGGCCAGGGGAGGTGCGCCTG
  GTGAGTTTGCGGCGTGCCAAGGCCCACGAGGGCTTGGGCTTCAGCATCCGTGGGGGCTCGGAGCACGGCG
  TGGGCATCTACGTGTCTCTGGTGGAACCAGGCTCTCTAGCTGAGAAGGAAGGACTGCGGGTCGGGGACCA
  GATTCTGCGCGTCAACGACAAATCCCTGGCCCGGGTGACCCACGCGGAGGCCGTCAAGGCTCTGAAGGGC
  TCCAAGAAGCTGGTGCTGTCTGTGTACTCAGCAGGGCGCATCCCTGGGGGCTACGTCACCAACCACATCT
  ACACCTGGGTGGACCCGCAGGGCCGCAGCATCTCCCCACCCTCGGGCCTGCCCCAGCCCCACGGTGGTGC
  CCTGAGGCAGCAGGAGGGTGACCGGAGGAGCACCCTGCACCTCCTGCAAGGAGGGGATGAGAAAAAGGTG
  AACCTGGTGCTGGGGGACGGCCGGTCCCTGGGCCTCACGATCCGTGGGGGAGCTGAGTACGGCCTTGGCA
  TTTACATCACTGGCGTGGACCCAGGCTCTGAAGCAGAAGGCAGCGGGCTCAAGGTTGGGGACCAGATTCT
  AGAAGTGAATGGGCGGAGCTTTCTCAACATCCTACACGACGAGGCTGTCAGGCTGCTTAAGTCATCTCGG
  CACCTCATCCTGACAGTGAAGGACGTCGGGAGGCTGCCCCATGCCCGCACCACTGTGGACGAGACCAAGT
  GGATCGCCAGTTCCCGGATCAGGGAGACCATGGCGAACTCGGCAGGGTTTCTTGGCGATCTCACAACAGA
  AGGAATAAACAAGCCAGGATTTTACAAGGGCCCAGCCGGCTCCCAGGTGACCCTGAGCAGCCTGGGGAAC
  CAGACACGAGTGCTGCTGGAGGAGCAGGCTCGGCACCTGCTGAACGAGCAGGAACACGCCACCATGGCCT
  ACTACCTGGATGAGTACCGTGGTGGCAGCGTCTCTGTGGAGGCCCTCGTCATGGCCCTGTTCAAGCTGCT
  CAACACCCACGCCAAGTTCTCACTCCTCTCTGAGGTGAGAGGCACCATTTCCCCGCAAGACCTAGAACGC
  TTCGACCACCTGGTGCTGAGGCGTGAGATTGAGTCCATGAAGGCGCGGCAGCCCCCAGGCCCCGGGGCTG
  GGGACACCTACTCCATGGTCTCCTACAGTGACACGGGTTCATCCACAGGCAGCCACGGCACCTCCACCAC
  CGTCAGCTCGGCCAGGAACACTCTGGACCTGGAGGAAACTGGCGAGGCTGTCCAGGGCAATATCAACGCC
  CTCCCAGATGTGTCCGTGGATGATGTCAGATCCACCTCCCAGGGGCTGTCAAGCTTCAAGCCACTGCCTC
  GCCCACCACCTCTGGCCCAAGGCAACGACCTCCCACTAGGCCAGCCAAGGAAGCTGGGGAGAGAGGACCT
  CCAGCCACCTTCCTCCATGCCTTCCTGCTCGGGCACTGTCTTCTCGGCTCCACAGAACCGCAGCCCGCCA
  GCGGGCACCGCACCCACCCCAGGGACCTCCTCTGCACAGGACTTGCCCTCTTCCCCCATCTATGCCTCCG
  TCTCCCCTGCCAACCCCAGCTCCAAGAGGCCGCTGGACGCCCATCTGGCCCTGGTCAACCAACACCCCAT
  CGGCCCCTTCCCACGGGTCCAGTCACCCCCGCACCTGAAAAGCCCCTCTGCAGAGGCCACAGTGGCTGGG
  GGCTGCCTTCTGCCCCCATCACCCTCTGGCCACCCAGACCAGACAGGCACAAACCAGCACTTTGTCATGG
  TGGAGGTCCACCGCCCCGACAGCGAGCCAGACGTCAATGAAGTGAGGGCGCTGCCCCAGACGCGCACAGC
  CTCTACGCTCTCCCAGCTCTCGGACAGCGGGCAGACTCTAAGCGAGGACAGTGGTGTGGATGCTGGCGAG
  GCAGAGGCCAGCGCCCCAGGCCGAGGAAGGCAGTCGGTGTCCACCAAGAGCAGGAGTAGCAAGGAGCTGC
  CTCGGAACGAGAGGCCCACAGATGGGGCCAACAAACCGCCTGGACTTCTGGAGCCCACGTCCACTCTGGT
  CCGTGTGAAGAAAAGTGCGGCCACCCTGGGCATCGCCATCGAGGGTGGCGCCAACACCCGCCAGCCCCTG
  CCTAGGATTGTCACTATTCAGAGAGGCGGCTCAGCTCACAACTGTGGGCAGCTCAAGGTGGGCCACGTGA
  TTCTGGAAGTGAATGGGCTGACGCTTCGGGGCAAGGAGCACCGGGAGGCCGCCCGCATTATCGCCGAGGC
  CTTCAAGACTAAGGACCGTGACTACATTGACTTTCTGGTCACTGAGTTCAATGTGATGCTC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220471 representing NM_015404
  Red=Cloning site Green=Tags(s)
  
  MNAPLDGLSVSSSSTGSLGSAAGAGGGGGAGLRLLSANVRQLHQALTALLSEAEREQFTHCLNAYHARRN
  VFDLVRTLRVLLDSPVKRRLLPMLRLVIPRSDQLLFDQYTAEGLYLPATTPYRQPAWGGPDSAGPGEVRL
  VSLRRAKAHEGLGFSIRGGSEHGVGIYVSLVEPGSLAEKEGLRVGDQILRVNDKSLARVTHAEAVKALKG
  SKKLVLSVYSAGRIPGGYVTNHIYTWVDPQGRSISPPSGLPQPHGGALRQQEGDRRSTLHLLQGGDEKKV
  NLVLGDGRSLGLTIRGGAEYGLGIYITGVDPGSEAEGSGLKVGDQILEVNGRSFLNILHDEAVRLLKSSR
  HLILTVKDVGRLPHARTTVDETKWIASSRIRETMANSAGFLGDLTTEGINKPGFYKGPAGSQVTLSSLGN
  QTRVLLEEQARHLLNEQEHATMAYYLDEYRGGSVSVEALVMALFKLLNTHAKFSLLSEVRGTISPQDLER
  FDHLVLRREIESMKARQPPGPGAGDTYSMVSYSDTGSSTGSHGTSTTVSSARNTLDLEETGEAVQGNINA
  LPDVSVDDVRSTSQGLSSFKPLPRPPPLAQGNDLPLGQPRKLGREDLQPPSSMPSCSGTVFSAPQNRSPP
  AGTAPTPGTSSAQDLPSSPIYASVSPANPSSKRPLDAHLALVNQHPIGPFPRVQSPPHLKSPSAEATVAG
  GCLLPPSPSGHPDQTGTNQHFVMVEVHRPDSEPDVNEVRALPQTRTASTLSQLSDSGQTLSEDSGVDAGE
  AEASAPGRGRQSVSTKSRSSKELPRNERPTDGANKPPGLLEPTSTLVRVKKSAATLGIAIEGGANTRQPL
  PRIVTIQRGGSAHNCGQLKVGHVILEVNGLTLRGKEHREAARIIAEAFKTKDRDYIDFLVTEFNVML
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_015404
• ORF Size: 2721 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_015404.1, NM_015404.2, NM_015404.3, NP_056219.1, NP_056219.3
• RefSeq Size: 4022
• RefSeq ORF: 2724
• Locus ID: 25861
• MW: 96.4 kDa
• Gene Summary: This gene is thought to function in the organization and stabilization of sterocilia elongation and actin cystoskeletal assembly, based on studies of the related mouse gene. Mutations in this gene have been associated with autosomal recessive non-syndromic deafness and Usher Syndrome. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Nov 2016]