FNIP1 (NM_133372) Human Tagged ORF Clone

CAT#: RC220543

FNIP1 (Myc-DDK-tagged)-Human folliculin interacting protein 1 (FNIP1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY408835) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220543 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: FNIP1
• Synonyms: DKFZp686E18167; DKFZp781P0215; KIAA1961; MGC667
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220543 representing NM_133372
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCCCTACGCTGTTCCAGAAGCTCTTCAGCAAGAGGACCGGGCTGGGCGCGCCCGGCCGCGACGCCC
  GGGACCCAGATTGCGGGTTCAGTTGGCCTTTACCAGAGTTTGATCCAAGCCAGATTCGACTGATTGTATA
  TCAAGACTGTGAAAGACGAGGGAGAAATGTTTTGTTTGACTCCAGTGTTAAGAGAAGAAATGAGGACATA
  TCAGTATCGAAACTCTGCAGTGATGCTCAAGTTAAAGTCTTTGGGAAATGCTGCCAACTGAAACCTGGAG
  GAGACAGTTCTTCCTCTTTAGATAGTTCTGTGACTTCATCTTCTGATATAAAAGACCAGTGTCTTAAGTA
  CCAGGGTTCTCGGTGCTCTTCTGATGCCAATATGCTTGGAGAGATGATGTTTGGCTCAGTAGCAATGAGC
  TACAAAGGATCCACCTTAAAAATTCATCAGATTCGTTCCCCTCCACAGCTCATGCTCAGCAAAGTGTTTA
  CTGCTCGGACTGGCAGCAGTATTTGTGGGAGTCTCAATACGCTACAAGATAGTCTTGAATTCATCAATCA
  GGACAACAATACATTAAAGGCTGATAATAACACAGTTATTAATGGACTGCTTGGAAATATAGGTCTTTCA
  CAGTTCTGCAGCCCCAGGCGGGCATTCTCTGAGCAGGGTCCGCTCCGCCTGATCAGGAGCGCCTCTTTCT
  TTGCAGTTCACAGCAACCCAATGGACATGCCTGGAAGAGAGCTCAATGAGGACAGAGACAGTGGCATAGC
  ACGGTCTGCATCTCTCAGCAGCTTGCTGATCACTCCATTTCCTTCCCCAAACTCCTCACTTACCCGAAGT
  TGTGCCAGCAGCTACCAGCGACGTTGGCGACGCAGCCAAACAACAAGTTTGGAAAATGGGGTATTTCCTA
  GATGGTCTATAGAAGAAAGCTTTAATCTCTCAGATGAAAGCTGTGGCCCTAACCCAGGAATTGTGCGGAA
  AAAGAAGATTGCAATTGGGGTAATCTTTTCATTGTCCAAAGATGAAGATGAAAATAACAAATTTAATGAA
  TTCTTTTTTTCACATTTTCCTCTCTTTGAAAGCCACATGAACAAATTAAAGAGTGCAATAGAACAGGCTA
  TGAAAATGAGCCGGAGATCAGCTGATGCCAGTCAGAGAAGTTTGGCATATAATCGAATAGTTGATGCCCT
  AAATGAATTCAGAACAACAATTTGTAATCTTTACACGATGCCACGAATTGGAGAACCTGTCTGGCTTACA
  ATGATGTCGGGGACTCCAGAAAAGAACCACCTTTGCTATCGTTTCATGAAGGAGTTCACCTTTCTAATGG
  AAAATGCTTCCAAAAATCAATTCTTGCCAGCTCTCATTACTGCAGTTCTGACCAATCATCTTGCCTGGGT
  TCCAACAGTCATGCCAAATGGACAACCACCTATAAAAATATTTTTAGAAAAGCATTCCTCTCAGAGTGTG
  GACATGTTGGCAAAGACTCATCCATATAACCCACTTTGGGCACAACTGGGAGACTTGTATGGCGCTATTG
  GCTCTCCCGTACGGTTAGCAAGGACTGTGGTAGTTGGCAAACGACAAGACATGGTCCAGAGGCTACTTTA
  TTTTCTTACTTATTTTATAAGATGCTCTGAACTTCAAGAAACGCATCTTTTAGAAAATGGAGAAGATGAA
  GCCATCGTTATGCCAGGCACAGTAATTACTACCACTTTAGAGAAAGGTGAAATAGAAGAATCAGAGTATG
  TCCTTGTCACAATGCATAGAAACAAAAGCAGTTTGCTCTTTAAAGAGTCAGAAGAAATTAGAACTCCCAA
  TTGTAACTGTAAATATTGCAGTCATCCACTCCTTGGGCAAAATGTAGAGAACATTTCACAACAAGAGAGA
  GAAGATATTCAAAACAGCTCTAAGGAGCTGCTAGGAATTTCAGATGAGTGCCAGATGATTTCTCCTTCTG
  ACTGCCAAGAAGAAAATGCTGTTGATGTTAAACAGTACAGAGATAAATTAAGAACTTGCTTTGACGCCAA
  GTTAGAGACAGTTGTTTGCACAGGATCTGTTCCAGTAGACAAATGTGCATTGTCAGAGTCAGGCTTAGAG
  TCAACAGAGGAAACATGGCAGAGTGAGAAGTTGCTGGATTCAGACAGTCACACAGGCAAAGCAATGAGAT
  CCACAGGAATGGTTGTGGAAAAAAAACCTCCAGATAAGATTGTGCCTGCTTCATTTTCTTGTGAGGCTGC
  CCAGACAAAGGTTACTTTCCTGATTGGGGATTCTATGTCACCTGATTCAGATACTGAGCTTCGAAGTCAG
  GCAGTGGTGGATCAGATTACCAGACATCACACCAAACCATTGAAGGAAGAAAGAGGGGCTATTGATCAGC
  ATCAAGAAACTAAACAAACAACCAAGGACCAATCTGGAGAGTCTGATACACAGAACATGGTTTCTGAAGA
  GCCCTGTGAACTTCCCTGTTGGAATCATTCAGACCCAGAAAGCATGAGCTTATTCGACGAATATTTTAAT
  GATGATTCAATCGAAACCAGGACTATTGATGATGTTCCATTTAAAACAAGTACAGATAGTAAAGACCATT
  GCTGTATGTTAGAGTTTTCAAAAATATTGTGTACAAAAAATAACAAGCAGAACAATGAATTTTGTAAATG
  TATAGAAACAGTTCCCCAAGATTCATGTAAAACCTGCTTTCCTCAGCAGGACCAAAGAGATACACTCTCC
  ATTCTTGTCCCCCATGGGGATAAAGAGAGTTCAGATAAAAAAATTGCTGTAGGAACTGAATGGGACATTC
  CAAGAAATGAAAGTTCAGACAGTGCCCTTGGGGATAGTGAAAGTGAAGATACAGGTCATGATATGACTAG
  ACAAGTTAGCAGTTATTATGGAGGAGAGCAAGAAGATTGGGCAGAAGAGGATGAGATACCTTTTCCTGGG
  TCAAAGTTAATCGAAGTGAGTGCTGTTCAGCCCAACATTGCCAACTTCGGGAGGTCCTTGCTGGGTGGCT
  ACTGCTCATCTTATGTGCCTGACTTTGTTCTTCAAGGAATTGGGAGTGATGAGAGGTTCCGTCAGTGTCT
  GATGTCAGATTTATCTCATGCTGTGCAGCATCCAGTTTTGGATGAACCAATAGCAGAAGCTGTCTGTATT
  ATAGCTGACATGGATAAATGGACTGTTCAAGTGGCCAGTAGCCAGAGACGAGTGACAGATAATAAATTGG
  GAAAGGAAGTATTGGTTTCCAGTCTTGTTTCCAATCTGCTTCATTCCACACTTCAGCTTTATAAGCATAA
  CTTGTCTCCAAATTTTTGTGTAATGCATCTTGAAGACCGGTTGCAGGAGCTATACTTCAAAAGTAAAATG
  CTGTCTGAATACCTGAGGGGGCAGATGCGTGTTCATGTCAAGGAGCTGGGAGTGGTTCTGGGGATTGAAT
  CCAGTGATCTTCCACTTCTGGCTGCTGTAGCAAGCACTCACTCTCCATATGTTGCACAAATACTCCTT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220543 representing NM_133372
  Red=Cloning site Green=Tags(s)
  
  MAPTLFQKLFSKRTGLGAPGRDARDPDCGFSWPLPEFDPSQIRLIVYQDCERRGRNVLFDSSVKRRNEDI
  SVSKLCSDAQVKVFGKCCQLKPGGDSSSSLDSSVTSSSDIKDQCLKYQGSRCSSDANMLGEMMFGSVAMS
  YKGSTLKIHQIRSPPQLMLSKVFTARTGSSICGSLNTLQDSLEFINQDNNTLKADNNTVINGLLGNIGLS
  QFCSPRRAFSEQGPLRLIRSASFFAVHSNPMDMPGRELNEDRDSGIARSASLSSLLITPFPSPNSSLTRS
  CASSYQRRWRRSQTTSLENGVFPRWSIEESFNLSDESCGPNPGIVRKKKIAIGVIFSLSKDEDENNKFNE
  FFFSHFPLFESHMNKLKSAIEQAMKMSRRSADASQRSLAYNRIVDALNEFRTTICNLYTMPRIGEPVWLT
  MMSGTPEKNHLCYRFMKEFTFLMENASKNQFLPALITAVLTNHLAWVPTVMPNGQPPIKIFLEKHSSQSV
  DMLAKTHPYNPLWAQLGDLYGAIGSPVRLARTVVVGKRQDMVQRLLYFLTYFIRCSELQETHLLENGEDE
  AIVMPGTVITTTLEKGEIEESEYVLVTMHRNKSSLLFKESEEIRTPNCNCKYCSHPLLGQNVENISQQER
  EDIQNSSKELLGISDECQMISPSDCQEENAVDVKQYRDKLRTCFDAKLETVVCTGSVPVDKCALSESGLE
  STEETWQSEKLLDSDSHTGKAMRSTGMVVEKKPPDKIVPASFSCEAAQTKVTFLIGDSMSPDSDTELRSQ
  AVVDQITRHHTKPLKEERGAIDQHQETKQTTKDQSGESDTQNMVSEEPCELPCWNHSDPESMSLFDEYFN
  DDSIETRTIDDVPFKTSTDSKDHCCMLEFSKILCTKNNKQNNEFCKCIETVPQDSCKTCFPQQDQRDTLS
  ILVPHGDKESSDKKIAVGTEWDIPRNESSDSALGDSESEDTGHDMTRQVSSYYGGEQEDWAEEDEIPFPG
  SKLIEVSAVQPNIANFGRSLLGGYCSSYVPDFVLQGIGSDERFRQCLMSDLSHAVQHPVLDEPIAEAVCI
  IADMDKWTVQVASSQRRVTDNKLGKEVLVSSLVSNLLHSTLQLYKHNLSPNFCVMHLEDRLQELYFKSKM
  LSEYLRGQMRVHVKELGVVLGIESSDLPLLAAVASTHSPYVAQILL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_133372
• ORF Size: 3498 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_133372.1, NM_133372.2, NP_588613.1, NP_588613.2
• RefSeq Size: 6584
• RefSeq ORF: 3501
• Locus ID: 96459
• MW: 130.4 kDa
• Gene Summary: This gene encodes a protein that binds to the tumor suppressor protein folliculin and to AMP-activated protein kinase (AMPK). The encoded protein participates in the regulation of cellular metabolism and nutrient sensing by modulating the AMPK and target of rapamycin signaling pathways. This gene has a closely related paralog that encodes a protein with similar binding activities. Both related proteins also associate with the molecular chaperone heat shock protein-90 (Hsp90) and negatively regulate its ATPase activity and facilitate its association with folliculin. [provided by RefSeq, Jul 2017]