Mineralocorticoid Receptor (NR3C2) (NM_000901) Human Tagged ORF Clone
CAT#: RC220558
NR3C2 (Myc-DDK-tagged)-Human nuclear receptor subfamily 3, group C, member 2 (NR3C2), transcript variant 1
"NM_000901" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | NR3C2 |
Synonyms | MCR; MLR; MR; NR3C2VIT |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC220558 representing NM_000901
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGACCAAAGGCTACCACAGTCTCCCTGAAGGTCTAGATATGGAAAGACGGTGGGGTCAAGTTTCTC AGGCTGTGGAGCGTTCTTCCCTGGGACCTACAGAGAGGACCGATGAGAATAACTACATGGAGATTGTCAA CGTAAGCTGTGTTTCCGGTGCTATTCCAAACAACAGTACTCAAGGAAGCAGCAAAGAAAAACAAGAACTA CTCCCTTGCCTTCAGCAAGACAATAATCGGCCTGGGATTTTAACATCTGATATTAAAACTGAGCTGGAAT CTAAGGAACTTTCAGCAACTGTAGCTGAGTCCATGGGTTTATATATGGATTCTGTAAGAGATGCTGACTA TTCCTATGAGCAGCAGAACCAACAAGGAAGCATGAGTCCAGCTAAGATTTATCAGAATGTTGAACAGCTG GTGAAATTTTACAAAGGAAATGGCCATCGTCCTTCCACTCTAAGTTGTGTGAACACGCCCTTGAGATCAT TTATGTCTGACTCTGGGAGCTCCGTGAATGGTGGCGTCATGCGCGCCGTTGTTAAAAGCCCTATCATGTG TCATGAGAAAAGCCCGTCTGTTTGCAGCCCTCTGAACATGACATCTTCGGTTTGCAGCCCTGCTGGAATC AACTCTGTGTCCTCCACCACAGCCAGCTTTGGCAGTTTTCCAGTGCACAGCCCAATCACCCAGGGAACTC CTCTGACATGCTCCCCTAATGTTGAAAATCGAGGCTCCAGGTCGCACAGCCCTGCACATGCTAGCAATGT GGGCTCTCCTCTCTCAAGTCCGTTAAGTAGCATGAAATCCTCAATTTCCAGCCCTCCAAGTCACTGCAGT GTAAAATCTCCAGTCTCCAGTCCCAATAATGTCACTCTGAGATCCTCTGTGTCTAGCCCTGCAAATATTA ACAACTCAAGGTGCTCTGTTTCCAGCCCTTCGAACACTAATAACAGATCCACGCTTTCCAGTCCGGCAGC CAGTACTGTGGGATCTATCTGTAGCCCTGTAAACAATGCCTTCAGCTACACTGCTTCTGGCACCTCTGCT GGATCCAGTACATTGCGGGATGTGGTTCCCAGTCCAGACACGCAGGAGAAAGGTGCTCAAGAGGTCCCTT TTCCTAAGACTGAGGAAGTAGAGAGTGCCATCTCAAATGGTGTGACTGGCCAGCTTAATATTGTCCAGTA CATAAAACCAGAACCAGATGGAGCTTTTAGCAGCTCATGTCTAGGAGGAAATAGCAAAATAAATTCGGAT TCTTCATTCTCAGTACCAATAAAGCAAGAATCAACCAAGCATTCATGTTCAGGCACCTCTTTTAAAGGGA ATCCAACAGTAAACCCGTTTCCATTTATGGATGGCTCGTATTTTTCCTTTATGGATGATAAAGACTATTA TTCCCTATCAGGAATTTTAGGACCACCTGTGCCCGGCTTTGATGGTAACTGTGAAGGCAGCGGATTCCCA GTGGGTATTAAACAAGAACCAGATGATGGGAGCTATTACCCAGAGGCCAGCATCCCTTCCTCTGCTATTG TTGGGGTGAATTCAGGTGGACAGTCCTTCCACTACAGGATTGGTGCTCAAGGTACAATATCTTTATCACG ATCGGCTAGAGACCAATCTTTCCAACACCTGAGTTCCTTTCCTCCTGTCAATACTTTAGTGGAGTCATGG AAATCACACGGCGACCTGTCGTCTAGAAGAAGTGATGGGTATCCGGTCTTAGAATACATTCCAGAAAATG TATCAAGCTCTACTTTACGAAGTGTTTCTACTGGATCTTCAAGACCTTCAAAAATATGTTTGGTGTGTGG GGATGAGGCTTCAGGATGCCATTATGGGGTAGTCACCTGTGGCAGCTGCAAAGTTTTCTTCAAAAGAGCA GTGGAAGGGCAACACAACTATTTATGTGCTGGAAGAAATGATTGCATCATTGATAAGATTCGACGAAAGA ATTGTCCTGCTTGCAGACTTCAGAAATGTCTTCAAGCTGGAATGAATTTAGGAGCACGAAAGTCAAAGAA GTTGGGAAAGTTAAAAGGGATTCACGAGGAGCAGCCACAGCAGCAGCAGCCCCCACCCCCACCCCCACCC CCGCAAAGCCCAGAGGAAGGGACAACGTACATCGCTCCTGCAAAAGAACCCTCGGTCAACACAGCACTGG TTCCTCAGCTCTCCACAATCTCACGAGCGCTCACACCTTCCCCCGTTATGGTCCTTGAAAACATTGAACC TGAAATTGTATATGCAGGCTATGACAGCTCAAAACCAGATACAGCCGAAAATCTGCTCTCCACGCTCAAC CGCTTAGCAGGCAAACAGATGATCCAAGTCGTGAAGTGGGCAAAGGTACTTCCAGGATTTAAAAACTTGC CTCTTGAGGACCAAATTACCCTAATCCAGTATTCTTGGATGTGTCTATCATCATTTGCCTTGAGCTGGAG ATCGTACAAACATACGAACAGCCAATTTCTCTATTTTGCACCAGACCTAGTCTTTAATGAAGAGAAGATG CATCAGTCTGCCATGTATGAACTATGCCAGGGGATGCACCAAATCAGCCTTCAGTTCGTTCGACTGCAGC TCACCTTTGAAGAATACACCATCATGAAAGTTTTGCTGCTACTAAGCACAATTCCAAAGGATGGCCTCAA AAGCCAGGCTGCATTTGAAGAAATGAGGACAAATTACATCAAAGAACTGAGGAAGATGGTAACTAAGTGT CCCAACAATTCTGGGCAGAGCTGGCAGAGGTTCTACCAACTGACCAAGCTGCTGGACTCCATGCATGACC TGGTGAGCGACCTGCTGGAATTCTGCTTCTACACCTTCCGAGAGTCCCATGCGCTGAAGGTAGAGTTCCC CGCAATGCTGGTGGAGATCATCAGCGACCAGCTGCCCAAGGTGGAGTCGGGGAACGCCAAGCCGCTCTAC TTCCACCGGAAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220558 representing NM_000901
Red=Cloning site Green=Tags(s) METKGYHSLPEGLDMERRWGQVSQAVERSSLGPTERTDENNYMEIVNVSCVSGAIPNNSTQGSSKEKQEL LPCLQQDNNRPGILTSDIKTELESKELSATVAESMGLYMDSVRDADYSYEQQNQQGSMSPAKIYQNVEQL VKFYKGNGHRPSTLSCVNTPLRSFMSDSGSSVNGGVMRAVVKSPIMCHEKSPSVCSPLNMTSSVCSPAGI NSVSSTTASFGSFPVHSPITQGTPLTCSPNVENRGSRSHSPAHASNVGSPLSSPLSSMKSSISSPPSHCS VKSPVSSPNNVTLRSSVSSPANINNSRCSVSSPSNTNNRSTLSSPAASTVGSICSPVNNAFSYTASGTSA GSSTLRDVVPSPDTQEKGAQEVPFPKTEEVESAISNGVTGQLNIVQYIKPEPDGAFSSSCLGGNSKINSD SSFSVPIKQESTKHSCSGTSFKGNPTVNPFPFMDGSYFSFMDDKDYYSLSGILGPPVPGFDGNCEGSGFP VGIKQEPDDGSYYPEASIPSSAIVGVNSGGQSFHYRIGAQGTISLSRSARDQSFQHLSSFPPVNTLVESW KSHGDLSSRRSDGYPVLEYIPENVSSSTLRSVSTGSSRPSKICLVCGDEASGCHYGVVTCGSCKVFFKRA VEGQHNYLCAGRNDCIIDKIRRKNCPACRLQKCLQAGMNLGARKSKKLGKLKGIHEEQPQQQQPPPPPPP PQSPEEGTTYIAPAKEPSVNTALVPQLSTISRALTPSPVMVLENIEPEIVYAGYDSSKPDTAENLLSTLN RLAGKQMIQVVKWAKVLPGFKNLPLEDQITLIQYSWMCLSSFALSWRSYKHTNSQFLYFAPDLVFNEEKM HQSAMYELCQGMHQISLQFVRLQLTFEEYTIMKVLLLLSTIPKDGLKSQAAFEEMRTNYIKELRKMVTKC PNNSGQSWQRFYQLTKLLDSMHDLVSDLLEFCFYTFRESHALKVEFPAMLVEIISDQLPKVESGNAKPLY FHRK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
![]() |
ACCN | NM_000901 |
ORF Size | 2952 bp |
OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_000901.1, NM_000901.2, NM_000901.3, NM_000901.4, NP_000892.1 |
RefSeq Size | 5749 bp |
RefSeq ORF | 2955 bp |
Locus ID | 4306 |
Cytogenetics | 4q31.23 |
Domains | HOLI, zf-C4 |
Protein Families | Druggable Genome, Nuclear Hormone Receptor, Transcription Factors |
MW | 106.9 kDa |
Gene Summary | 'This gene encodes the mineralocorticoid receptor, which mediates aldosterone actions on salt and water balance within restricted target cells. The protein functions as a ligand-dependent transcription factor that binds to mineralocorticoid response elements in order to transactivate target genes. Mutations in this gene cause autosomal dominant pseudohypoaldosteronism type I, a disorder characterized by urinary salt wasting. Defects in this gene are also associated with early onset hypertension with severe exacerbation in pregnancy. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2009]' |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC123888 | NR3C2 (untagged)-Human nuclear receptor subfamily 3, group C, member 2 (NR3C2), transcript variant 1 |
USD 2,189.00 |
|
RG220558 | NR3C2 (GFP-tagged) - Human nuclear receptor subfamily 3, group C, member 2 (NR3C2), transcript variant 1 |
USD 1,130.00 |
|
RC220558L1 | Lenti ORF clone of Human nuclear receptor subfamily 3, group C, member 2 (NR3C2), transcript variant 1, Myc-DDK-tagged |
USD 1,524.00 |
|
RC220558L2 | Lenti ORF clone of Human nuclear receptor subfamily 3, group C, member 2 (NR3C2), transcript variant 1, mGFP tagged |
USD 1,230.00 |
|
RC220558L3 | Lenti ORF clone of Human nuclear receptor subfamily 3, group C, member 2 (NR3C2), transcript variant 1, Myc-DDK-tagged |
USD 1,230.00 |
|
RC220558L4 | Lenti ORF clone of Human nuclear receptor subfamily 3, group C, member 2 (NR3C2), transcript variant 1, mGFP tagged |
USD 1,230.00 |
{0} Product Review(s)
Be the first one to submit a review