CHREBP (MLXIPL) (NM_032951) Human Tagged ORF Clone

CAT#: RC220626

MLXIPL (Myc-DDK-tagged)-Human MLX interacting protein-like (MLXIPL), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY409836) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220626 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MLXIPL
• Synonyms: bHLHd14; CHREBP; MIO; MLX; MONDOB; WBSCR14; WS-bHLH
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220626 representing NM_032951
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCCGGCGCGCTGGCAGGTCTGGCCGCGGGCTTGCAGGTCCCGCGGGTCGCGCCCAGCCCAGACTCGG
  ACTCGGACACAGACTCGGAGGACCCGAGTCTCCGGCGCAGCGCGGGCGGCTTGCTCCGCTCGCAGGTCAT
  CCACAGCGGTCACTTCATGGTGTCGTCGCCGCACAGCGACTCGCTGCCCCGGCGGCGCGACCAGGAGGGG
  TCCGTGGGGCCCTCCGACTTCGGGCCGCGCAGTATCGACCCCACACTCACACGCCTCTTCGAGTGCTTGA
  GCCTGGCCTACAGTGGCAAGCTGGTGTCTCCCAAGTGGAAGAATTTCAAAGGCCTCAAGCTGCTCTGCAG
  AGACAAGATCCGCCTGAACAACGCCATCTGGAGGGCCTGGTATATCCAGTATGTGAAGCGGAGGAAGAGC
  CCCGTGTGTGGCTTCGTGACCCCCCTGCAGGGGCCTGAGGCTGATGCGCACCGGAAGCCGGAGGCCGTGG
  TCCTGGAGGGGAACTACTGGAAGCGGCGCATCGAGGTGGTGATGCGGGAATACCACAAGTGGCGCATCTA
  CTACAAGAAGCGGCTCCGTAAGCCCAGCAGGGAAGATGACCTCCTGGCCCCTAAGCAGGCGGAAGGCAGG
  TGGCCGCCGCCGGAGCAATGGTGCAAACAGCTCTTCTCCAGTGTGGTCCCCGTGCTGCTGGGGGACCCAG
  AGGAGGAGCCGGGTGGGCGGCAGCTCCTGGACCTCAATTGCTTTTTGTCCGACATCTCAGACACTCTCTT
  CACCATGACTCAGTCCGGCCCTTCGCCCCTGCAGCTGCCGCCTGAGGATGCCTACGTCGGCAATGCTGAC
  ATGATCCAGCCGGACCTGACGCCACTGCAGCCAAGCCTGGATGACTTCATGGACATCTCAGATTTCTTTA
  CCAACTCCCGCCTCCCACAGCCGCCCATGCCTTCAAACTTCCCAGAGCCCCCCAGCTTCAGCCCCGTGGT
  TGACTCCCTCTTCAGCAGTGGGACCCTGGGCCCAGAGGTGCCCCCGGCTTCCTCGGCCATGACCCACCTC
  TCTGGACACAGCCGTCTGCAGGCTCGGAACAGCTGCCCTGGCCCCTTGGACTCCAGCGCCTTCCTGAGTT
  CTGATTTCCTCCTTCCTGAAGACCCCAAGCCCCGGCTCCCACCCCCTCCTGTACCCCCACCTCTGCTGCA
  TTACCCTCCCCCTGCCAAGGTGCCAGGCCTGGAGCCCTGCCCCCCACCTCCCTTCCCTCCCATGGCACCA
  CCCACTGCTTTGCTGCAGGAAGAGCCTCTCTTCTCTCCCAGGTTTCCCTTCCCCACCGTCCCTCCTGCCC
  CAGGAGTGTCTCCGCTGCCTGCTCCTGCAGCCTTCCCACCCACCCCACAGTCTGTCCCCAGCCCAGCCCC
  CACCCCCTTCCCCATAGAGCTTCTACCCTTGGGGTATTCGGAGCCTGCCTTTGGGCCTTGCTTCTCCATG
  CCCAGAGGCAAGCCCCCCGCCCCATCCCCTAGGGGACAGAAAGCCAGCCCCCCTACCTTAGCCCCTGCCA
  CTGCCAGTCCCCCCACCACTGCGGGGAGCAACAACCCCTGCCTCACACAGCTGCTCACAGCAGCTAAGCC
  GGAGCAAGCCCTGGAGCCACCACTTGTATCCAGCACCCTCCTCCGGTCCCCAGGGTCCCCGCAGGAGACA
  GTCCCTGAATTCCCCTGCACATTCCTTCCCCCGACCCCGGCCCCTACACCGCCCCGGCCACCTCCAGGCC
  CGGCCACATTGGCCCCTTCCAGGCCCCTGCTTGTCCCCAAAGCGGAGCGGCTCTCACCCCCAGCGCCCAG
  CGGCAGTGAACGGCGGCTGTCAGGGGACCTCAGCTCCATGCCAGGCCCTGGGACTCTGAGCGTCCGTGTC
  TCTCCCCCGCAACCCATCCTCAGCCGGGGCCGTCCAGACAGCAACAAGACCGAGAACCGGCGTATCACAC
  ACATCTCCGCGGAGCAGAAGCGGCGCTTCAACATCAAGCTGGGGTTTGACACCCTTCATGGGCTCGTGAG
  CACACTCAGTGCCCAGCCCAGCCTCAAGGTGAGCAAAGCTACCACGCTGCAGAAGACAGCTGAGTACATC
  CTTATGCTACAGCAGGAGCGTGCGGGCTTGCAGGAGGAGGCCCAGCAGCTGCGGGATGAGATTGAGGAGC
  TCAATGCCGCCATTAACCTGTGCCAGCAGCAGCTGCCCGCCACAGGGGTACCCATCACACACCAGCGTTT
  TGACCAGATGCGAGACATGTTTGATGACTACGTCCGAACCCGTACGCTGCACAACTGGAAGTTCTGGGTG
  TTCAGCATCCTCATCCGGCCTCTGTTTGAGTCCTTCAACGGGATGGTGTCCACGGCAAGTGTGCACACCC
  TCCGCCAGACCTCACTGGCCTGGCTGGACCAGTACTGCTCTCTGCCCGCTCTCCGGCCAACTGTCCTGAA
  CTCCCTACGCCAGCTGGGCACATCTACCAGTATCCTGACCGACCCGGGCCGCATCCCTGAGCAAGCCACA
  CGGGCAGTCACAGAGGGCACCCTTGGCAAACCTTTA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220626 representing NM_032951
  Red=Cloning site Green=Tags(s)
  
  MAGALAGLAAGLQVPRVAPSPDSDSDTDSEDPSLRRSAGGLLRSQVIHSGHFMVSSPHSDSLPRRRDQEG
  SVGPSDFGPRSIDPTLTRLFECLSLAYSGKLVSPKWKNFKGLKLLCRDKIRLNNAIWRAWYIQYVKRRKS
  PVCGFVTPLQGPEADAHRKPEAVVLEGNYWKRRIEVVMREYHKWRIYYKKRLRKPSREDDLLAPKQAEGR
  WPPPEQWCKQLFSSVVPVLLGDPEEEPGGRQLLDLNCFLSDISDTLFTMTQSGPSPLQLPPEDAYVGNAD
  MIQPDLTPLQPSLDDFMDISDFFTNSRLPQPPMPSNFPEPPSFSPVVDSLFSSGTLGPEVPPASSAMTHL
  SGHSRLQARNSCPGPLDSSAFLSSDFLLPEDPKPRLPPPPVPPPLLHYPPPAKVPGLEPCPPPPFPPMAP
  PTALLQEEPLFSPRFPFPTVPPAPGVSPLPAPAAFPPTPQSVPSPAPTPFPIELLPLGYSEPAFGPCFSM
  PRGKPPAPSPRGQKASPPTLAPATASPPTTAGSNNPCLTQLLTAAKPEQALEPPLVSSTLLRSPGSPQET
  VPEFPCTFLPPTPAPTPPRPPPGPATLAPSRPLLVPKAERLSPPAPSGSERRLSGDLSSMPGPGTLSVRV
  SPPQPILSRGRPDSNKTENRRITHISAEQKRRFNIKLGFDTLHGLVSTLSAQPSLKVSKATTLQKTAEYI
  LMLQQERAGLQEEAQQLRDEIEELNAAINLCQQQLPATGVPITHQRFDQMRDMFDDYVRTRTLHNWKFWV
  FSILIRPLFESFNGMVSTASVHTLRQTSLAWLDQYCSLPALRPTVLNSLRQLGTSTSILTDPGRIPEQAT
  RAVTEGTLGKPL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_032951
• ORF Size: 2556 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_032951.1, NM_032951.2, NP_116569.1
• RefSeq Size: 3288 bp
• RefSeq ORF: 2559 bp
• Locus ID: 51085
• MW: 92.9 kDa
• Gene Summary: This gene encodes a basic helix-loop-helix leucine zipper transcription factor of the Myc/Max/Mad superfamily. This protein forms a heterodimeric complex and binds and activates, in a glucose-dependent manner, carbohydrate response element (ChoRE) motifs in the promoters of triglyceride synthesis genes. The gene is deleted in Williams-Beuren syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at chromosome 7q11.23. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Dec 2015]