EBF2 (NM_022659) Human Tagged ORF Clone

CAT#: RC220630

EBF2 (Myc-DDK-tagged)-Human early B-cell factor 2 (EBF2)

Product Images

Western blot validation of overexpression lysate (Cat# LY411603) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220630 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified EBF2 protein (Cat# TP320630). The protein was produced from HEK293T cells transfected with EBF2 cDNA clone (Cat# RC220630) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: EBF2
• Synonyms: COE2; EBF-2; O/E-3; OE-3
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220630 representing NM_022659
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTTTGGAATTCAAGATACTTTAGGAAGAGGACCAACTCTGAAAGAGAAATCGCTGGGCGCGGAGATGG
  ATTCGGTCAGGTCCTGGGTCCGGAATGTCGGAGTGGTGGACGCTAATGTCGCCGCGCAGAGCGGGGTCGC
  CCTGTCCCGGGCCCACTTTGAGAAACAGCCTCCTTCCAACTTGAGGAAATCCAACTTCTTTCACTTCGTC
  CTGGCGCTCTATGACAGGCAGGGCCAGCCGGTGGAGATCGAGCGGACGGCCTTCGTGGACTTTGTGGAGA
  ATGACAAAGAACAAGGCAACGAGAAGACCAACAACGGCACTCACTACAAGTTACAGCTCCTCTACAGCAA
  CGGTGTCCGCACGGAACAGGACCTCTATGTCAGGCTCATCGACTCGGTCACCAAGCAGCCCATCGCTTAC
  GAGGGACAGAATAAGAATCCGGAAATGTGCCGAGTTCTCCTGACGCACGAAGTGATGTGTAGTCGATGCT
  GCGAAAAGAAAAGCTGTGGAAACCGAAATGAGACTCCATCGGACCCAGTCATAATTGACAGATTCTTTTT
  AAAATTTTTCCTCAAGTGCAATCAGAATTGTTTGAAAACAGCAGGAAACCCAAGGGACATGAGACGGTTT
  CAGGTTGTGTTGTCAACAACGGTGAATGTGGATGGACACGTCCTGGCTGTTTCTGACAACATGTTTGTTC
  ATAACAACTCCAAGCATGGACGGAGAGCAAGAAGACTCGATCCATCGGAAGCTACCCCCTGCATCAAAGC
  CATTAGCCCGAGTGAAGGCTGGACCACAGGAGGAGCCATGGTCATCATCATCGGGGACAACTTCTTTGAT
  GGTCTCCAAGTGGTGTTTGGGACTATGCTTGTATGGAGCGAGCTAATAACCCCTCATGCCATCAGAGTAC
  AGACTCCTCCCCGGCACATCCCAGGCGTGGTAGAGGTGACATTATCTTATAAATCTAAACAGTTCTGCAA
  AGGAGCCCCAGGAAGGTTCATTTACACAGCATTAAATGAACCCACCATAGACTATGGCTTCCAGAGACTG
  CAGAAGGTCATCCCTAGGCATCCTGGAGATCCTGAGAGATTAGCTAAGGAGATGCTGTTGAAAAGAGCTG
  CAGATCTAGTGGAAGCTCTTTATGGCACACCACACAATAACCAGGACATCATTTTGAAGCGAGCCGCAGA
  CATTGCTGAAGCTCTCTACAGCGTCCCCAGGAATCCCAGCCAGCTTCCAGCCCTCTCTAGCTCCCCAGCG
  CACAGTGGCATGATGGGAATCAACTCCTATGGCAGCCAGCTTGGGGTCAGCATCTCAGAGTCAACACAAG
  GAAATAATCAAGGGTACATCCGCAACACAAGCAGCATCTCTCCGCGGGGATACTCTTCCAGCTCCACGCC
  TCAACAGTCTAATTACAGTACCTCCAGCAACAGTATGAATGGCTACAGCAATGTCCCCATGGCCAACTTG
  GGTGTTCCAGGTTCACCAGGATTTCTAAATGGCTCACCCACCGGCTCTCCTTATGGAATCATGTCATCAA
  GTCCCACCGTTGGGTCTTCCAGCACATCCTCCATCCTCCCATTTTCCTCTTCAGTTTTTCCTGCTGTCAA
  ACAGAAGAGTGCCTTTGCCCCTGTCATCAGGCCCCAAGGCTCCCCTTCACCTGCCTGCTCCAGCGGCAAT
  GGAAATGGATTCAGAGCCATGACCGGACTTGTTGTACCCCCGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220630 representing NM_022659
  Red=Cloning site Green=Tags(s)
  
  MFGIQDTLGRGPTLKEKSLGAEMDSVRSWVRNVGVVDANVAAQSGVALSRAHFEKQPPSNLRKSNFFHFV
  LALYDRQGQPVEIERTAFVDFVENDKEQGNEKTNNGTHYKLQLLYSNGVRTEQDLYVRLIDSVTKQPIAY
  EGQNKNPEMCRVLLTHEVMCSRCCEKKSCGNRNETPSDPVIIDRFFLKFFLKCNQNCLKTAGNPRDMRRF
  QVVLSTTVNVDGHVLAVSDNMFVHNNSKHGRRARRLDPSEATPCIKAISPSEGWTTGGAMVIIIGDNFFD
  GLQVVFGTMLVWSELITPHAIRVQTPPRHIPGVVEVTLSYKSKQFCKGAPGRFIYTALNEPTIDYGFQRL
  QKVIPRHPGDPERLAKEMLLKRAADLVEALYGTPHNNQDIILKRAADIAEALYSVPRNPSQLPALSSSPA
  HSGMMGINSYGSQLGVSISESTQGNNQGYIRNTSSISPRGYSSSSTPQQSNYSTSSNSMNGYSNVPMANL
  GVPGSPGFLNGSPTGSPYGIMSSSPTVGSSSTSSILPFSSSVFPAVKQKSAFAPVIRPQGSPSPACSSGN
  GNGFRAMTGLVVPPM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_022659
• ORF Size: 1725 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_022659.2, NM_022659.3, NP_073150.2
• RefSeq Size: 2297
• RefSeq ORF: 1728
• Locus ID: 64641
• MW: 62.5 kDa
• Gene Summary: The protein encoded by this gene belongs to the COE (Collier/Olf/EBF) family of non-basic, helix-loop-helix transcription factors that have a well conserved DNA binding domain. The COE family proteins play an important role in variety of developmental processes. Studies in mouse suggest that this gene may be involved in the differentiation of osteoblasts. [provided by RefSeq, Oct 2011]