ACSM1 (NM_052956) Human Tagged ORF Clone

CAT#: RC220646

ACSM1 (Myc-DDK-tagged)-Human acyl-CoA synthetase medium-chain family member 1 (ACSM1)

Product Images

Western blot validation of overexpression lysate (Cat# LY403276) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220646 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ACSM1
• Synonyms: BUCS1; MACS1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220646 representing NM_052956
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGCAGTGGCTAATGAGGTTCCGGACCCTCTGGGGCATCCACAAATCCTTCCACAACATCCACCCTGCCC
  CTTCACAGCTGCGCTGCCGGTCTTTATCAGAATTTGGAGCCCCAAGATGGAATGACTATGAAGTACCGGA
  GGAATTTAACTTTGCAAGTTATGTACTGGACTACTGGGCTCAAAAGGAGAAGGAGGGCAAGAGAGGTCCA
  AATCCAGCTTTTTGGTGGGTGAATGGCCAAGGGGATGAAGTAAAGTGGAGCTTCAGAGAGATGGGAGACC
  TAACCCGCCGTGTAGCCAACGTCTTCACACAGACCTGTGGCCTACAACAGGGAGACCATCTGGCCTTGAT
  GCTGCCTCGTGTTCCTGAGTGGTGGCTGGTGGCTGTGGGCTGCATGCGAACAGGGATCATCTTCATTCCT
  GCGACCATCCTGTTGAAGGCCAAAGACATTCTCTATCGACTACAGTTGTCTAAAGCCAAGGGCATTGTGA
  CCATAGATGCCCTTGCCTCAGAGGTGGACTCCATAGCTTCTCAGTGCCCCTCTCTGAAAACCAAGCTCCT
  GGTGTCTGATCACAGCCGTGAAGGGTGGCTGGACTTCCGATCGCTGGTTAAATCAGCATCCCCAGAACAC
  ACCTGTGTTAAGTCAAAGACCTTGGACCCAATGGTCATCTTCTTCACCAGTGGGACCACAGGCTTCCCCA
  AGATGGCAAAACACTCCCATGGGTTGGCCTTACAACCCTCCTTCCCAGGAAGTAGGAAATTACGGAGCCT
  GAAGACATCTGATGTCTCCTGGTGCCTGTCGGACTCAGGATGGATTGTGGCTACCATTTGGACCCTGGTA
  GAACCATGGACAGCGGGTTGTACAGTCTTTATCCACCATCTGCCACAGTTTGACACCAAGGTCATCATAC
  AGACATTGTTGAAATACCCCATTAACCACTTTTGGGGGGTATCATCTATATATCGAATGATTCTGCAGCA
  GGATTTCACCAGCATCAGGTTCCCTGCCCTGGAGCACTGCTATACTGGCGGGGAGGTCGTGTTGCCCAAG
  GATCAGGAGGAGTGGAAAAGACGGACGGGCCTTCTGCTCTACGAGAACTATGGGCAGTCGGAAACGGGAC
  TAATTTGTGCCACCTACTGGGGAATGAAGATCAAGCCGGGTTTCATGGGGAAGGCCACTCCACCCTATGA
  CGTCCAGGTCATTGATGACAAGGGCAGCATCCTGCCACCTAACACAGAAGGAAACATTGGCATCAGAATC
  AAACCTGTCAGGCCTGTGAGCCTCTTCATGTGCTATGAGGGTGACCCAGAGAAGACAGCTAAAGTGGAAT
  GTGGGGACTTCTACAACACTGGGGACAGAGGAAAGATGGATGAAGAGGGCTACATTTGTTTCCTGGGGAG
  GAGTGATGACATCATTAATGCCTCTGGGTATCGCATCGGGCCTGCAGAGGTTGAAAGCGCTTTGGTGGAG
  CACCCAGCGGTGGCGGAGTCAGCCGTGGTGGGCAGCCCAGACCCGATTCGAGGGGAGGTGGTGAAGGCCT
  TTATTGTCCTGACCCCACAGTTCCTGTCCCATGACAAGGATCAGCTGACCAAGGAACTGCAGCAGCATGT
  CAAGTCAGTGACAGCCCCATACAAGTACCCAAGGAACGTGGAGTTTGTCTCAGAGCTGCCAAAAACCATC
  ACTGGCAAGATTGAACGGAAGGAACTTCGGAAAAAGGAGACTGGTCAGATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220646 representing NM_052956
  Red=Cloning site Green=Tags(s)
  
  MQWLMRFRTLWGIHKSFHNIHPAPSQLRCRSLSEFGAPRWNDYEVPEEFNFASYVLDYWAQKEKEGKRGP
  NPAFWWVNGQGDEVKWSFREMGDLTRRVANVFTQTCGLQQGDHLALMLPRVPEWWLVAVGCMRTGIIFIP
  ATILLKAKDILYRLQLSKAKGIVTIDALASEVDSIASQCPSLKTKLLVSDHSREGWLDFRSLVKSASPEH
  TCVKSKTLDPMVIFFTSGTTGFPKMAKHSHGLALQPSFPGSRKLRSLKTSDVSWCLSDSGWIVATIWTLV
  EPWTAGCTVFIHHLPQFDTKVIIQTLLKYPINHFWGVSSIYRMILQQDFTSIRFPALEHCYTGGEVVLPK
  DQEEWKRRTGLLLYENYGQSETGLICATYWGMKIKPGFMGKATPPYDVQVIDDKGSILPPNTEGNIGIRI
  KPVRPVSLFMCYEGDPEKTAKVECGDFYNTGDRGKMDEEGYICFLGRSDDIINASGYRIGPAEVESALVE
  HPAVAESAVVGSPDPIRGEVVKAFIVLTPQFLSHDKDQLTKELQQHVKSVTAPYKYPRNVEFVSELPKTI
  TGKIERKELRKKETGQM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_052956
• ORF Size: 1731 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_052956.1, NM_052956.2, NP_443188.1
• RefSeq Size: 1945 bp
• RefSeq ORF: 1734 bp
• Locus ID: 116285
• Protein Pathways: Butanoate metabolism, Metabolic pathways
• MW: 65.1 kDa