RIOK1 (NM_031480) Human Tagged ORF Clone
CAT#: RC220667
RIOK1 (Myc-DDK-tagged)-Human RIO kinase 1 (yeast) (RIOK1), transcript variant 1
"NM_031480" in other vectors (6)
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Symbol | RIOK1 |
Synonyms | AD034; bA288G3.1; RIO1; RRP10 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC220667 representing NM_031480
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTACCGGCGGCTTCTCATGAGCCGGGTGGTCCCCGGGCAATTCGACGACGCGGACTCCTCTGACA GTGAAAACAGAGACTTGAAGACAGTCAAAGAGAAGGATGACATTCTGTTTGAAGACCTTCAAGACAATGT GAATGAGAATGGTGAAGGTGAAATAGAAGATGAGGAGGAGGAGGGTTATGATGATGATGATGATGACTGG GACTGGGATGAAGGAGTTGGAAAACTCGCCAAGGGTTATGTCTGGAATGGAGGAAGCAACCCACAGGCAA ATCGACAGACCTCCGACAGCAGTTCAGCCAAAATGTCTACTCCAGCAGACAAGGTCTTACGGAAATTTGA GAATAAAATTAATTTAGATAAGCTAAATGTTACTGATTCCGTCATAAATAAAGTCACCGAAAAGTCTAGA CAAAAGGAAGCAGATATGTATCGCATCAAAGATAAGGCAGACAGAGCAACTGTAGAACAGGTGTTGGATC CCAGAACAAGAATGATTTTATTCAAGATGTTGACTAGAGGAATCATAACAGAGATAAATGGCTGCATTAG CACAGGAAAAGAAGCTAATGTATACCATGCTAGCACAGCAAATGGAGAGAGCAGAGCAATCAAAATTTAT AAAACTTCTATTTTGGTGTTCAAAGATCGGGATAAATATGTAAGTGGAGAATTCAGATTTCGTCATGGCT ATTGTAAAGGAAACCCTAGGAAAATGGTGAAAACTTGGGCAGAAAAAGAAATGAGGAACTTAATCAGGCT AAACACAGCAGAGATACCATGTCCAGAACCAATAATGCTAAGAAGTCATGTTCTTGTCATGAGTTTCATC GGTAAAGATGACATGCCTGCACCACTCTTGAAAAATGTCCAGTTATCAGAATCCAAGGCTCGGGAGTTGT ACCTGCAGGTCATTCAGTACATGAGAAGAATGTATCAGGATGCCAGACTTGTCCATGCAGATCTCAGTGA ATTTAACATGCTGTACCACGGTGGAGGCGTGTATATCATTGACGTGTCTCAGTCCGTGGAGCACGACCAC CCACATGCCTTGGAGTTCTTGAGAAAGGATTGCGCCAACGTCAATGATTTCTTTATGAGGCACAGTGTTG CTGTCATGACTGTGCGGGAGCTCTTTGAATTTGTCACAGATCCATCCATTACACATGAGAACATGGATGC TTATCTCTCAAAGGCCATGGAAATAGCATCTCAAAGGACCAAGGAAGAACGGTCTAGCCAAGATCATGTG GATGAAGAGGTGTTTAAGCGAGCATATATTCCTAGAACCTTGAATGAAGTGAAAAATTATGAGAGGGATA TGGACATAATTATGAAATTGAAGGAAGAGGACATGGCCATGAATGCCCAACAAGATAATATTCTATACCA GACTGTTACAGGATTGAAGAAAGATTTGTCAGGAGTTCAGAAGGTCCCTGCACTCCTAGAAAATCAAGTG GAGGAAAGGACTTGTTCTGATTCAGAAGATATTGGAAGCTCTGAGTGCTCTGACACAGACTCTGAAGAGC AGGGAGACCATGCCCGCCCCAAGAAACACACCACGGACCCTGACATTGATAAAAAAGAAAGAAAAAAGAT GGTCAAGGAAGCCCAGAGAGAGAAAAGAAAAAACAAAATTCCTAAACATGTGAAAAAAAGAAAGGAGAAG ACAGCCAAGACGAAAAAAGGCAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC220667 representing NM_031480
Red=Cloning site Green=Tags(s) MDYRRLLMSRVVPGQFDDADSSDSENRDLKTVKEKDDILFEDLQDNVNENGEGEIEDEEEEGYDDDDDDW DWDEGVGKLAKGYVWNGGSNPQANRQTSDSSSAKMSTPADKVLRKFENKINLDKLNVTDSVINKVTEKSR QKEADMYRIKDKADRATVEQVLDPRTRMILFKMLTRGIITEINGCISTGKEANVYHASTANGESRAIKIY KTSILVFKDRDKYVSGEFRFRHGYCKGNPRKMVKTWAEKEMRNLIRLNTAEIPCPEPIMLRSHVLVMSFI GKDDMPAPLLKNVQLSESKARELYLQVIQYMRRMYQDARLVHADLSEFNMLYHGGGVYIIDVSQSVEHDH PHALEFLRKDCANVNDFFMRHSVAVMTVRELFEFVTDPSITHENMDAYLSKAMEIASQRTKEERSSQDHV DEEVFKRAYIPRTLNEVKNYERDMDIIMKLKEEDMAMNAQQDNILYQTVTGLKKDLSGVQKVPALLENQV EERTCSDSEDIGSSECSDTDSEEQGDHARPKKHTTDPDIDKKERKKMVKEAQREKRKNKIPKHVKKRKEK TAKTKKGK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_031480 |
ORF Size | 1704 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers. |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_031480.2, NP_113668.2 |
RefSeq Size | 2495 bp |
RefSeq ORF | 1707 bp |
Locus ID | 83732 |
Domains | RIO |
Protein Families | Druggable Genome, Protein Kinase |
MW | 65.4 kDa |
Gene Summary | The protein encoded by this gene competes with pICln for inclusion in the protein arginine methyltransferase 5 complex. This complex targets substrates for dimethylation. The encoded protein is essential for the last steps in the maturation of 40S subunits. [provided by RefSeq, Jan 2017] |
Documents
Product Manuals |
FAQs |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
SC110673 | RIOK1 (untagged)-Human RIO kinase 1 (yeast) (RIOK1), transcript variant 1 |
USD 310.00 |
|
RG220667 | RIOK1 (GFP-tagged) - Human RIO kinase 1 (yeast) (RIOK1), transcript variant 1 |
USD 460.00 |
|
RC220667L1 | Lenti ORF clone of Human RIO kinase 1 (yeast) (RIOK1), transcript variant 1, Myc-DDK-tagged |
USD 768.00 |
|
RC220667L2 | Lenti ORF clone of Human RIO kinase 1 (yeast) (RIOK1), transcript variant 1, mGFP tagged |
USD 620.00 |
|
RC220667L3 | Lenti ORF clone of Human RIO kinase 1 (yeast) (RIOK1), transcript variant 1, Myc-DDK-tagged |
USD 620.00 |
|
RC220667L4 | Lenti ORF clone of Human RIO kinase 1 (yeast) (RIOK1), transcript variant 1, mGFP tagged |
USD 620.00 |
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