RIOK1 (NM_031480) Human Tagged ORF Clone

CAT#: RC220667

RIOK1 (Myc-DDK-tagged)-Human RIO kinase 1 (yeast) (RIOK1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY410475) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220667 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified RIOK1 protein (Cat# TP320667). The protein was produced from HEK293T cells transfected with RIOK1 cDNA clone (Cat# RC220667) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: RIOK1
• Synonyms: AD034; bA288G3.1; RIO1; RRP10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220667 representing NM_031480
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACTACCGGCGGCTTCTCATGAGCCGGGTGGTCCCCGGGCAATTCGACGACGCGGACTCCTCTGACA
  GTGAAAACAGAGACTTGAAGACAGTCAAAGAGAAGGATGACATTCTGTTTGAAGACCTTCAAGACAATGT
  GAATGAGAATGGTGAAGGTGAAATAGAAGATGAGGAGGAGGAGGGTTATGATGATGATGATGATGACTGG
  GACTGGGATGAAGGAGTTGGAAAACTCGCCAAGGGTTATGTCTGGAATGGAGGAAGCAACCCACAGGCAA
  ATCGACAGACCTCCGACAGCAGTTCAGCCAAAATGTCTACTCCAGCAGACAAGGTCTTACGGAAATTTGA
  GAATAAAATTAATTTAGATAAGCTAAATGTTACTGATTCCGTCATAAATAAAGTCACCGAAAAGTCTAGA
  CAAAAGGAAGCAGATATGTATCGCATCAAAGATAAGGCAGACAGAGCAACTGTAGAACAGGTGTTGGATC
  CCAGAACAAGAATGATTTTATTCAAGATGTTGACTAGAGGAATCATAACAGAGATAAATGGCTGCATTAG
  CACAGGAAAAGAAGCTAATGTATACCATGCTAGCACAGCAAATGGAGAGAGCAGAGCAATCAAAATTTAT
  AAAACTTCTATTTTGGTGTTCAAAGATCGGGATAAATATGTAAGTGGAGAATTCAGATTTCGTCATGGCT
  ATTGTAAAGGAAACCCTAGGAAAATGGTGAAAACTTGGGCAGAAAAAGAAATGAGGAACTTAATCAGGCT
  AAACACAGCAGAGATACCATGTCCAGAACCAATAATGCTAAGAAGTCATGTTCTTGTCATGAGTTTCATC
  GGTAAAGATGACATGCCTGCACCACTCTTGAAAAATGTCCAGTTATCAGAATCCAAGGCTCGGGAGTTGT
  ACCTGCAGGTCATTCAGTACATGAGAAGAATGTATCAGGATGCCAGACTTGTCCATGCAGATCTCAGTGA
  ATTTAACATGCTGTACCACGGTGGAGGCGTGTATATCATTGACGTGTCTCAGTCCGTGGAGCACGACCAC
  CCACATGCCTTGGAGTTCTTGAGAAAGGATTGCGCCAACGTCAATGATTTCTTTATGAGGCACAGTGTTG
  CTGTCATGACTGTGCGGGAGCTCTTTGAATTTGTCACAGATCCATCCATTACACATGAGAACATGGATGC
  TTATCTCTCAAAGGCCATGGAAATAGCATCTCAAAGGACCAAGGAAGAACGGTCTAGCCAAGATCATGTG
  GATGAAGAGGTGTTTAAGCGAGCATATATTCCTAGAACCTTGAATGAAGTGAAAAATTATGAGAGGGATA
  TGGACATAATTATGAAATTGAAGGAAGAGGACATGGCCATGAATGCCCAACAAGATAATATTCTATACCA
  GACTGTTACAGGATTGAAGAAAGATTTGTCAGGAGTTCAGAAGGTCCCTGCACTCCTAGAAAATCAAGTG
  GAGGAAAGGACTTGTTCTGATTCAGAAGATATTGGAAGCTCTGAGTGCTCTGACACAGACTCTGAAGAGC
  AGGGAGACCATGCCCGCCCCAAGAAACACACCACGGACCCTGACATTGATAAAAAAGAAAGAAAAAAGAT
  GGTCAAGGAAGCCCAGAGAGAGAAAAGAAAAAACAAAATTCCTAAACATGTGAAAAAAAGAAAGGAGAAG
  ACAGCCAAGACGAAAAAAGGCAAA
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220667 representing NM_031480
  Red=Cloning site Green=Tags(s)
  
  MDYRRLLMSRVVPGQFDDADSSDSENRDLKTVKEKDDILFEDLQDNVNENGEGEIEDEEEEGYDDDDDDW
  DWDEGVGKLAKGYVWNGGSNPQANRQTSDSSSAKMSTPADKVLRKFENKINLDKLNVTDSVINKVTEKSR
  QKEADMYRIKDKADRATVEQVLDPRTRMILFKMLTRGIITEINGCISTGKEANVYHASTANGESRAIKIY
  KTSILVFKDRDKYVSGEFRFRHGYCKGNPRKMVKTWAEKEMRNLIRLNTAEIPCPEPIMLRSHVLVMSFI
  GKDDMPAPLLKNVQLSESKARELYLQVIQYMRRMYQDARLVHADLSEFNMLYHGGGVYIIDVSQSVEHDH
  PHALEFLRKDCANVNDFFMRHSVAVMTVRELFEFVTDPSITHENMDAYLSKAMEIASQRTKEERSSQDHV
  DEEVFKRAYIPRTLNEVKNYERDMDIIMKLKEEDMAMNAQQDNILYQTVTGLKKDLSGVQKVPALLENQV
  EERTCSDSEDIGSSECSDTDSEEQGDHARPKKHTTDPDIDKKERKKMVKEAQREKRKNKIPKHVKKRKEK
  TAKTKKGK
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_031480
• ORF Size: 1704 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_031480.2, NP_113668.2
• RefSeq Size: 2495 bp
• RefSeq ORF: 1707 bp
• Locus ID: 83732
• Domains: RIO
• Protein Families: Druggable Genome, Protein Kinase
• MW: 65.4 kDa
• Gene Summary: The protein encoded by this gene competes with pICln for inclusion in the protein arginine methyltransferase 5 complex. This complex targets substrates for dimethylation. The encoded protein is essential for the last steps in the maturation of 40S subunits. [provided by RefSeq, Jan 2017]