ZNF33A (NM_006954) Human Tagged ORF Clone

CAT#: RC220676

ZNF33A (Myc-DDK-tagged)-Human zinc finger protein 33A (ZNF33A), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: ZNF33A
• Synonyms: KOX2; KOX5; KOX31; NF11A; ZNF11; ZNF11A; ZNF33; ZZAPK
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220676 representing NM_006954
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAACAAGGTAGAACAGAAGTCCCAGGAGTCAGTATCATTTAAAGATGTGACTGTGGGCTTCACCCAGG
  AGGAGTGGCAGCACCTGGACCCTAGTCAGAGGGCTCTGTATAGAGATGTGATGCTGGAGAACTACAGCAA
  CCTTGTCTCAGTGGGGTATTGTGTTCACAAACCAGAGGTGATCTTCAGGCTGCAACAAGGAGAAGAGCCA
  TGGAAACAGGAGGAAGAATTCCCAAGCCAAAGCTTTCCAGAAGTCTGGACAGCTGATCACCTGAAAGAGA
  GGAGCCAAGAAAACCAATCTAAACATTTGTGGGAAGTTGTATTCATCAATAATGAAATGCTGACTAAGGA
  ACAAGGTGATGTAATAGGAATACCATTTAATGTGGATGTAAGTTCTTTTCCTTCCAGAAAAATGTTCTGT
  CAGTGTGATTCATGTGGAATGAGTTTCAACACTGTTTCAGAATTGGTTATCAGTAAGATAAACTATTTAG
  GAAAAAAGTCTGATGAATTTAATGCCTGTGGGAAATTGTTACTCAATATTAAGCATGATGAAACTCATAC
  TCAAGAGAAAAATGAAGTTTTGAAAAATAGGAACACACTGAGTCATCATGAGGAGACTTTGCAGCATGAG
  AAGATTCAAACTTTAGAGCACAATTTTGAATACAGTATATGTCAGGAAACCCTCCTTGAAAAGGCAGTAT
  TCAATACACAGAAGAGAGAGAACGCAGAAGAGAATAACTGTGATTATAATGAATTTGGGAGAACTTTGTG
  TGATAGTTCATCCCTCTTGTTCCATCAGATATCTCCGTCAAGGGACAATCACTATGAATTTAGTGATTGT
  GAGAAGTTCTTATGTGTGAAGTCCACCCTTTCTAAACCTCATGGGGTATCTATGAAACACTATGATTGTG
  GTGAAAGTGGGAATAATTTCAGGAGGAAATTGTGTCTGTCACACCTTCAGAAAGGTGATAAAGGAGAGAA
  ACACTTTGAATGTAATGAATGTGGGAAAGCTTTCTGGGAGAAGTCACATCTCACTCGACATCAGAGGGTG
  CACACAGGACAGAAACCCTTTCAATGTAATGAATGTGAAAAAGCTTTCTGGGATAAGTCAAACCTCACTA
  AACATCAAAGATCACACACAGGGGAGAAACCTTTTGAATGCAATGAATGTGGGAAAGCCTTTAGCCATAA
  GTCAGCCCTCACATTACACCAGAGAACACATACAGGGGAGAAACCCTATCAATGTAATGCGTGTGGGAAA
  ACTTTTTGCCAGAAATCTGACCTCACTAAACATCAGAGAACACACACAGGGCTGAAACCCTATGAATGTT
  ATGAATGTGGAAAATCCTTCCGTGTGACTTCGCACCTTAAAGTACACCAGAGAACTCACACAGGTGAGAA
  ACCTTTTGAATGTCTTGAGTGTGGGAAATCCTTTAGTGAAAAGTCAAATCTTACACAGCATCAGAGAATT
  CACATAGGAGATAAATCTTATGAATGTAATGCATGTGGGAAAACTTTCTACCACAAGTCATTACTCACCA
  GGCATCAGATAATTCATACAGGGTGGAAACCTTATGAATGTTATGAATGTGGGAAAACCTTCTGCTTGAA
  GTCAGACCTCACAGTACATCAGAGAACACACACAGGGCAGAAACCCTTTGCATGTCCCGAATGTGGGAAA
  TTCTTTAGCCATAAGTCAACCCTCTCTCAACATTATAGAACACACACAGGGGAGAAACCCTACGAATGTC
  ATGAATGTGGAAAAATCTTTTACAATAAATCATACCTAACTAAACATAATAGAACACATACAGGGGAGAA
  ACCCTATGAATGTAATGAATGTGGAAAAGCCTTCTACCAGAAGTCACAACTCACTCAGCATCAGAGAATT
  CACATAGGGGAGAAACCCTATAAATGTAATGAGTGTGGAAAAGCTTTCTGCCATAAGTCAGCTCTAATTG
  TACATCAGAGAACCCATACACAAGAAAAGCCCTATAAATGTAATGAATGTGGAAAATCTTTCTGTGTAAA
  ATCAGGACTTATTTTCCATGAGAGAAAGCACACGGGGGAGAAACCCTATGAATGCAATGAATGTGGGAAA
  TTCTTCAGGCACAAATCATCACTCACAGTACATCACAGGGCTCACACAGGAGAGAAATCTTGTCAATGTA
  ATGAATGTGGAAAAATCTTTTACCGTAAATCGGAACTTGCTCAACATCAGAGATCACATACAGGGGAAAA
  GCCCTATGAATGTAACACATGCAGGAAAACTTTCTCTCAAAAGTCAAATCTCATTGTACATCAGAGAAGA
  CATATAGGAGAAAACCTTATGAATGAAATGGATATTAGAAATTTCCAGCCACAAGTCAGCCTCCATAATG
  CCTCAGAGTATTCACACTGTGGAGAAAGCCCTGATGACATCCTGAATGTTCAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220676 representing NM_006954
  Red=Cloning site Green=Tags(s)
  
  MNKVEQKSQESVSFKDVTVGFTQEEWQHLDPSQRALYRDVMLENYSNLVSVGYCVHKPEVIFRLQQGEEP
  WKQEEEFPSQSFPEVWTADHLKERSQENQSKHLWEVVFINNEMLTKEQGDVIGIPFNVDVSSFPSRKMFC
  QCDSCGMSFNTVSELVISKINYLGKKSDEFNACGKLLLNIKHDETHTQEKNEVLKNRNTLSHHEETLQHE
  KIQTLEHNFEYSICQETLLEKAVFNTQKRENAEENNCDYNEFGRTLCDSSSLLFHQISPSRDNHYEFSDC
  EKFLCVKSTLSKPHGVSMKHYDCGESGNNFRRKLCLSHLQKGDKGEKHFECNECGKAFWEKSHLTRHQRV
  HTGQKPFQCNECEKAFWDKSNLTKHQRSHTGEKPFECNECGKAFSHKSALTLHQRTHTGEKPYQCNACGK
  TFCQKSDLTKHQRTHTGLKPYECYECGKSFRVTSHLKVHQRTHTGEKPFECLECGKSFSEKSNLTQHQRI
  HIGDKSYECNACGKTFYHKSLLTRHQIIHTGWKPYECYECGKTFCLKSDLTVHQRTHTGQKPFACPECGK
  FFSHKSTLSQHYRTHTGEKPYECHECGKIFYNKSYLTKHNRTHTGEKPYECNECGKAFYQKSQLTQHQRI
  HIGEKPYKCNECGKAFCHKSALIVHQRTHTQEKPYKCNECGKSFCVKSGLIFHERKHTGEKPYECNECGK
  FFRHKSSLTVHHRAHTGEKSCQCNECGKIFYRKSELAQHQRSHTGEKPYECNTCRKTFSQKSNLIVHQRR
  HIGENLMNEMDIRNFQPQVSLHNASEYSHCGESPDDILNVQ
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_006954
• ORF Size: 2433 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_006954.1, NP_008885.1
• RefSeq Size: 6130 bp
• RefSeq ORF: 2436 bp
• Locus ID: 7581
• Cytogenetics: 10p11.1
• Protein Families: Transcription Factors, Transmembrane
• MW: 94.3 kDa
• Gene Summary: ''