MMP20 (NM_004771) Human Tagged ORF Clone

CAT#: RC220786

MMP20 (Myc-DDK-tagged)-Human matrix metallopeptidase 20 (MMP20)

Product Images

Western blot validation of overexpression lysate (Cat# LY417767) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220786 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MMP20
• Synonyms: AI2A2; MMP-20
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220786 representing NM_004771
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAAGGTGCTCCCTGCATCTGGCCTTGCTGTCTTCCTCATCATGGCTTTGAAGTTTTCCACTGCAGCCC
  CCTCCCTAGTTGCAGCCTCCCCCAGGACCTGGAGGAACAACTACCGCCTCGCACAGGCGTATCTTGACAA
  ATATTACACAAATAAAGAAGGACACCAGATTGGTGAGATGGTTGCAAGAGGAAGCAATTCCATGATAAGG
  AAGATTAAGGAGCTACAAGCGTTCTTTGGCCTCCAAGTCACCGGGAAGTTAGACCAGACCACAATGAACG
  TGATCAAGAAGCCTCGCTGTGGAGTTCCTGATGTGGCCAATTATCGCCTCTTCCCTGGTGAACCCAAATG
  GAAAAAAAATACTTTGACATACAGAATATCTAAATACACACCTTCCATGAGTTCTGTCGAGGTGGACAAA
  GCAGTGGAGATGGCCTTGCAGGCCTGGAGTAGCGCCGTCCCTCTGAGCTTTGTCAGAATAAACTCAGGAG
  AAGCGGATATTATGATATCTTTTGAAAATGGAGATCACGGGGATTCCTATCCATTCGATGGGCCTCGGGG
  GACTCTAGCCCATGCATTTGCTCCTGGAGAAGGCCTGGGAGGAGATACACATTTCGACAATGCTGAGAAG
  TGGACTATGGGAACGAATGGTTTTAATTTGTTTACCGTTGCTGCTCATGAATTTGGCCATGCCCTGGGCC
  TGGCCCATTCCACAGACCCATCAGCACTGATGTACCCAACTTATAAGTACAAGAATCCCTATGGATTCCA
  CCTCCCCAAAGATGATGTGAAAGGGATCCAGGCATTATACGGACCTCGGAAAGTATTCCTGGGGAAGCCC
  ACTCTGCCCCATGCCCCCCATCACAAGCCATCCATCCCTGACCTCTGTGACTCCAGCTCATCCTTTGACG
  CTGTGACAATGCTGGGGAAGGAGCTCCTGCTCTTCAAGGACCGGATTTTCTGGAGACGGCAGGTTCACTT
  GCGGACAGGAATTCGGCCCAGCACTATTACCAGCTCCTTCCCCCAGCTCATGTCCAATGTGGATGCAGCT
  TACGAAGTGGCTGAGAGGGGCACTGCTTACTTCTTCAAAGGTCCCCACTACTGGATAACAAGAGGATTCC
  AAATGCAAGGTCCTCCTCGGACTATTTATGACTTTGGATTTCCAAGGCACGTGCAGCAAATAGATGCTGC
  TGTCTACCTCAGGGAGCCACAGAAGACCCTTTTCTTTGTGGGAGATGAATACTACAGCTACGACGAAAGG
  AAAAGGAAAATGGAAAAAGACTATCCAAAGAATACTGAAGAAGAATTTTCAGGAGTAAATGGCCAAATCG
  ATGCTGCTGTAGAATTAAATGGCTACATTTACTTCTTTTCAGGACCAAAAACATACAAGTATGACACAGA
  GAAGGAAGATGTGGTTAGTGTGGTGAAATCTAGTTCCTGGATTGGTTGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220786 representing NM_004771
  Red=Cloning site Green=Tags(s)
  
  MKVLPASGLAVFLIMALKFSTAAPSLVAASPRTWRNNYRLAQAYLDKYYTNKEGHQIGEMVARGSNSMIR
  KIKELQAFFGLQVTGKLDQTTMNVIKKPRCGVPDVANYRLFPGEPKWKKNTLTYRISKYTPSMSSVEVDK
  AVEMALQAWSSAVPLSFVRINSGEADIMISFENGDHGDSYPFDGPRGTLAHAFAPGEGLGGDTHFDNAEK
  WTMGTNGFNLFTVAAHEFGHALGLAHSTDPSALMYPTYKYKNPYGFHLPKDDVKGIQALYGPRKVFLGKP
  TLPHAPHHKPSIPDLCDSSSSFDAVTMLGKELLLFKDRIFWRRQVHLRTGIRPSTITSSFPQLMSNVDAA
  YEVAERGTAYFFKGPHYWITRGFQMQGPPRTIYDFGFPRHVQQIDAAVYLREPQKTLFFVGDEYYSYDER
  KRKMEKDYPKNTEEEFSGVNGQIDAAVELNGYIYFFSGPKTYKYDTEKEDVVSVVKSSSWIGC
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_004771
• ORF Size: 1449 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_004771.1, NM_004771.2, NM_004771.3, NP_004762.2
• RefSeq Size: 1956 bp
• RefSeq ORF: 1452 bp
• Locus ID: 9313
• Protein Families: Druggable Genome, Secreted Protein, Transmembrane
• MW: 54.39 kDa
• Gene Summary: Proteins of the matrix metalloproteinase (MMP) family are involved in the breakdown of extracellular matrix in normal physiological processes, such as embryonic development, reproduction, and tissue remodeling, as well as in disease processes, such as arthritis and metastasis. Most MMP's are secreted as inactive proproteins which are activated when cleaved by extracellular proteinases. The protein encoded by this gene degrades amelogenin, the major protein component of dental enamel matrix, and thus thought to play a role in tooth enamel formation. A mutation in this gene, which alters the normal splice pattern and results in premature termination of the encoded protein, has been associated with amelogenesis imperfecta. This gene is part of a cluster of MMP genes located on chromosome 11q22.3. [provided by RefSeq, Aug 2011]