USP16 (NM_001032410) Human Tagged ORF Clone

CAT#: RC220829

USP16 (Myc-DDK-tagged)-Human ubiquitin specific peptidase 16 (USP16), transcript variant 3

Product Images

Western blot validation of overexpression lysate (Cat# LY416633) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC222609 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: USP16
• Synonyms: UBP-M; UBPM
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220829 ORF sequence
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAAAGAAACGGACAAAGGGAAAAACTGTTCCAATCGATGATTCCTCTGAAACTTTAGAACCTGTGT
  GCAGACACATTAGAAAAGGATTGGAACAAGGTAATTTGAAAAAGGCTTTAGTGAATGTGGAATGGAATAT
  CTGCCAAGACTGTAAGACTGACAATAAAGTGAAAGATAAAGCTGAAGAAGAAACAGAAGAAAAGCCTTCA
  GTTTGGCTGTGTCTTAAATGTGGCCATCAGGGCTGTGGCAGAAATTCTCAGGAGCAGCATGCCTTGAAGC
  ACTATCTGACGCCAAGATCTGAACCTCACTGTCTGGTTCTTAGTTTGGACAACTGGAGTGTATGGTGTTA
  CGTATGTGATAATGAGGTCCAGTATTGTAGTTCAAACCAGTTGGGTCAAGTGGTTGATTATGTCAGAAAA
  CATGCCAGCATTACAACTCCAAAGCCAGAGAAAGATAATGGAAATATTGAACTTGAAAATAAAAAATTAG
  AAAAAGAGAGTAAGAATGAACAAGAGAGAGAAAAGAAGGAAAACATGGCTAAAGAGAATCCTCCCATGAA
  TTCTCCTTGCCAAATAACCGTGAAAGGACTCAGTAATTTGGGAAACACATGTTTCTTCAATGCAGTTATG
  CAGAACTTGTCACAAACACCAGTGCTTAGAGAACTACTAAAAGAAGTGAAAATGTCTGGAACAATTGTAA
  AAATTGAACCACCTGATTTGGCATTAACAGAACCATTAGAAATAAACCTTGAGCCTCCAGGCCCTCTTAC
  TTTAGCCATGAGCCAGTTTCTTAATGAGATGCAAGAGACCAAAAAGGGGGTTGTGACACCGAAAGAACTC
  TTTTCTCAGGTCTGTAAAAAAGCAGTGCGGTTTAAAGGCTATCAGCAGCAAGACAGCCAGGAGCTGCTTC
  GCTACTTATTGGATGGGATGAGAGCAGAAGAACACCAAAGAGTGAGTAAAGGAATACTTAAAGCATTTGG
  TAATTCTACTGAAAAGTTGGATGAAGAACTAAAAAATAAAGTTAAAGATTATGAGAAGAAAAAATCAATG
  CCAAGTTTTGTTGACCGCATCTTTGGTGGTGAACTAACTAGTATGATCATGTGTGATCAATGCAGAACTG
  TCTCCTTGGTTCATGAATCTTTCCTTGATTTGTCCCTCCCAGTTTTAGATGATCAGAGTGGTAAGAAAAG
  TGTAAATGATAAAAATCTGAAAAAGACAGTGGAGGATGAAGATCAAGATAGTGAGGAAGAAAAAGATAAC
  GACAGTTACATAAAAGAGAGAAGTGATATTCCTTCTGGAACAAGTAAGCACTTACAGAAAAAAGCAAAGA
  AACAAGCCAAAAAGCAAGCCAAGAACCAACGAAGACAACAAAAAATTCAAGGAAAAGTTCTTCATTTAAA
  TGATATTTGTACTATTGACCATCCTGAAGACAGTGATAATGAAGCTGAAATGTCACTTCAAGGAGAAGTA
  AATATTAAATCCAACCATATTTCACAAGAGGGTGTTATGCATAAAGAATATTGTGTCAACCAGAAAGATT
  TGAATGGCCAAGCAAAAATGATCGAAAGTGTAACTGACAATCAAAAATCCACAGAGGAAGTAGATATGAA
  AAATATCAACATGGATAATGATCTGGAGGTTTTAACATCTTCTCCCACTAGGAATTTAAATGGTGCCTAC
  CTAACGGAAGGGAGCAATGGAGAAGTGGACATTTCCAATGGTTTCAAAAACCTAAATTTGAATGCTGCTC
  TTCATCCTGATGAAATAAATATAGAGATTCTGAATGATAGTCATACTCCTGGAACAAAGGTGTATGAGGT
  TGTAAATGAAGATCCAGAAACTGCTTTCTGTACTCTTGCAAACAGGGAAGTTTTCAATACTGATGAGTGT
  TCAATCCAACATTGTTTATATCAGTTCACCCGTAATGAGAAACTTCGAGATGCGAATAAACTGCTTTGTG
  AAGTATGCACACGGAGACAGTGTAATGGACCAAAGGCAAATATAAAAGGTGAAAGGAAGCATGTTTACAC
  CAATGCCAAAAAGCAGATGCTAATTTCTCTTGCTCCTCCTGTTCTTACTCTTCATTTAAAGAGATTTCAG
  CAGGCTGGTTTTAACCTACGCAAAGTTAACAAACACATAAAGTTTCCGGAAATCTTAGATTTGGCTCCTT
  TTTGCACCCTTAAATGTAAGAATGTTGCAGAAGAAAATACAAGGGTACTCTATTCCTTATATGGAGTTGT
  TGAACACAGTGGTACTATGAGGTCGGGGCATTACACTGCCTATGCCAAGGCAAGAACCGCAAATAGTCAT
  CTCTCTAATCTTGTTCTTCACGGTGATATTCCACAAGATTTTGAAATGGAATCAAAAGGGCAGTGGTTTC
  ACATCAGCGACACACATGTGCAAGCTGTGCCTACAACTAAAGTACTAAACTCACAAGCGTACCTCCTATT
  TTATGAGAGAATACTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220829 protein sequence
  Red=Cloning site Green=Tags(s)
  
  MGKKRTKGKTVPIDDSSETLEPVCRHIRKGLEQGNLKKALVNVEWNICQDCKTDNKVKDKAEEETEEKPS
  VWLCLKCGHQGCGRNSQEQHALKHYLTPRSEPHCLVLSLDNWSVWCYVCDNEVQYCSSNQLGQVVDYVRK
  HASITTPKPEKDNGNIELENKKLEKESKNEQEREKKENMAKENPPMNSPCQITVKGLSNLGNTCFFNAVM
  QNLSQTPVLRELLKEVKMSGTIVKIEPPDLALTEPLEINLEPPGPLTLAMSQFLNEMQETKKGVVTPKEL
  FSQVCKKAVRFKGYQQQDSQELLRYLLDGMRAEEHQRVSKGILKAFGNSTEKLDEELKNKVKDYEKKKSM
  PSFVDRIFGGELTSMIMCDQCRTVSLVHESFLDLSLPVLDDQSGKKSVNDKNLKKTVEDEDQDSEEEKDN
  DSYIKERSDIPSGTSKHLQKKAKKQAKKQAKNQRRQQKIQGKVLHLNDICTIDHPEDSDNEAEMSLQGEV
  NIKSNHISQEGVMHKEYCVNQKDLNGQAKMIESVTDNQKSTEEVDMKNINMDNDLEVLTSSPTRNLNGAY
  LTEGSNGEVDISNGFKNLNLNAALHPDEINIEILNDSHTPGTKVYEVVNEDPETAFCTLANREVFNTDEC
  SIQHCLYQFTRNEKLRDANKLLCEVCTRRQCNGPKANIKGERKHVYTNAKKQMLISLAPPVLTLHLKRFQ
  QAGFNLRKVNKHIKFPEILDLAPFCTLKCKNVAEENTRVLYSLYGVVEHSGTMRSGHYTAYAKARTANSH
  LSNLVLHGDIPQDFEMESKGQWFHISDTHVQAVPTTKVLNSQAYLLFYERIL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_001032410
• ORF Size: 1910 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_001032410.1, NP_001027582.1
• RefSeq Size: 3052 bp
• RefSeq ORF: 2472 bp
• Locus ID: 10600
• Cytogenetics: 21q21.3
• Protein Families: Protease
• MW: 93.4 kDa
• Gene Summary: This gene encodes a deubiquitinating enzyme that is phosphorylated at the onset of mitosis and then dephosphorylated at the metaphase/anaphase transition. It can deubiquitinate H2A, one of two major ubiquitinated proteins of chromatin, in vitro and a mutant form of the protein was shown to block cell division. Alternate transcriptional splice variants, encoding different isoforms, have been characterized. [provided by RefSeq, Jul 2008]