GART (NM_175085) Human Tagged ORF Clone

CAT#: RC220898

GART (Myc-DDK-tagged)-Human phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase (GART), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY406318) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220898 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified GART protein (Cat# TP320898). The protein was produced from HEK293T cells transfected with GART cDNA clone (Cat# RC220898) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: GART
• Synonyms: AIRS; GARS; GARTF; PAIS; PGFT; PRGS
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220898 representing NM_175085
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCAGCCCGAGTACTTATAATTGGCAGTGGAGGAAGGGAACATACGCTGGCCTGGAAACTTGCACAGT
  CTCATCATGTCAAACAAGTGTTGGTTGCCCCAGGAAACGCAGGCACTGCCTGCTCTGAAAAGATTTCAAA
  TACCGCCATCTCAATCAGTGACCACACTGCCCTTGCTCAATTCTGCAAAGAGAAGAAAATTGAATTTGTA
  GTTGTTGGACCAGAAGCACCTCTGGCTGCTGGGATTGTTGGGAACCTGAGGTCTGCAGGAGTGCAATGCT
  TTGGCCCAACAGCAGAAGCGGCTCAGTTAGAGTCCAGCAAAAGGTTTGCCAAAGAGTTTATGGACAGACA
  TGGAATCCCAACCGCACAATGGAAGGCTTTCACCAAACCTGAAGAAGCCTGCAGCTTCATTTTGAGTGCA
  GACTTCCCTGCTTTGGTTGTGAAGGCCAGTGGTCTTGCAGCTGGAAAAGGGGTGATTGTTGCAAAGAGCA
  AAGAAGAGGCCTGCAAAGCTGTACAAGAGATCATGCAGGAGAAAGCCTTTGGGGCAGCTGGAGAAACAAT
  TGTCATTGAAGAACTTCTTGACGGAGAAGAGGTGTCGTGTCTGTGTTTCACTGATGGCAAGACTGTGGCC
  CCCATGCCCCCAGCACAGGACCATAAGCGATTACTGGAGGGAGATGGTGGCCCTAACACAGGGGGAATGG
  GAGCCTATTGTCCAGCCCCTCAGGTTTCTAATGATCTATTACTAAAAATTAAAGATACTGTTCTTCAGAG
  GACAGTGGATGGCATGCAGCAAGAGGGTACTCCATATACAGGTATTCTCTATGCTGGAATAATGCTGACC
  AAGAATGGCCCAAAAGTTCTAGAGTTTAATTGCCGTTTTGGTGATCCAGAGTGCCAAGTAATCCTCCCAC
  TTCTTAAAAGTGATCTTTATGAAGTGATTCAGTCCACCTTAGATGGACTGCTCTGCACATCTCTGCCTGT
  TTGGCTAGAAAACCACACCGCCCTAACTGTTGTCATGGCAAGTAAAGGTTATCCTGGAGACTACACCAAG
  GGTGTAGAGATAACAGGGTTTCCTGAGGCTCAAGCTCTAGGACTGGAGGTGTTCCATGCAGGCACTGCCC
  TCAAAAATGGCAAAGTAGTAACTCATGGGGGTAGAGTTCTTGCAGTCACAGCCATCCGGGAAAATCTCAT
  ATCAGCCCTTGAGGAAGCCAAGAAAGGACTAGCTGCTATAAAGTTTGAGGGAGCAATTTATAGGAAAGAC
  GTCGGCTTTCGTGCCATAGCTTTCCTCCAGCAGCCCAGG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220898 representing NM_175085
  Red=Cloning site Green=Tags(s)
  
  MAARVLIIGSGGREHTLAWKLAQSHHVKQVLVAPGNAGTACSEKISNTAISISDHTALAQFCKEKKIEFV
  VVGPEAPLAAGIVGNLRSAGVQCFGPTAEAAQLESSKRFAKEFMDRHGIPTAQWKAFTKPEEACSFILSA
  DFPALVVKASGLAAGKGVIVAKSKEEACKAVQEIMQEKAFGAAGETIVIEELLDGEEVSCLCFTDGKTVA
  PMPPAQDHKRLLEGDGGPNTGGMGAYCPAPQVSNDLLLKIKDTVLQRTVDGMQQEGTPYTGILYAGIMLT
  KNGPKVLEFNCRFGDPECQVILPLLKSDLYEVIQSTLDGLLCTSLPVWLENHTALTVVMASKGYPGDYTK
  GVEITGFPEAQALGLEVFHAGTALKNGKVVTHGGRVLAVTAIRENLISALEEAKKGLAAIKFEGAIYRKD
  VGFRAIAFLQQPR
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_175085
• ORF Size: 1299 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_175085.1, NM_175085.2, NP_780294.1
• RefSeq Size: 2162 bp
• RefSeq ORF: 1302 bp
• Locus ID: 2618
• Cytogenetics: 21q22.11
• Protein Pathways: Metabolic pathways, One carbon pool by folate, Purine metabolism
• MW: 45.9 kDa
• Gene Summary: 'The protein encoded by this gene is a trifunctional polypeptide. It has phosphoribosylglycinamide formyltransferase, phosphoribosylglycinamide synthetase, phosphoribosylaminoimidazole synthetase activity which is required for de novo purine biosynthesis. This enzyme is highly conserved in vertebrates. Alternative splicing of this gene results in two transcript variants encoding different isoforms. [provided by RefSeq, Jul 2008]'