KIAA1967 (CCAR2) (NM_021174) Human Tagged ORF Clone

CAT#: RC220900

KIAA1967 (Myc-DDK-tagged)-Human KIAA1967 (KIAA1967), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY412049) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220900 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified CCAR2 protein (Cat# TP320900). The protein was produced from HEK293T cells transfected with CCAR2 cDNA clone (Cat# RC220900) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: CCAR2
• Synonyms: DBC-1; DBC1; KIAA1967; NET35; p30 DBC; p30DBC
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220900 representing NM_021174
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCCCAGTTTAAGCGCCAGCGGATCAACCCGCTTCCAGGGGGACGCAACTTCTCAGGCACAGCTTCAA
  CATCTCTTCTGGGCCCTCCTCCTGGTTTGCTCACTCCTCCTGTGGCCACAGAACTGTCCCAGAATGCCAG
  GCACCTTCAGGGTGGGGAGAAACAGCGGGTCTTCACTGGTATTGTTACCAGCTTGCATGACTACTTTGGG
  GTTGTGGATGAAGAGGTCTTTTTTCAGCTAAGTGTGGTGAAGGGCCGTCTGCCCCAGCTGGGTGAGAAGG
  TGCTGGTGAAGGCTGCATACAACCCAGGCCAGGCAGTGCCCTGGAATGCTGTCAAGGTGCAAACGCTCTC
  CAACCAGCCCCTACTGAAGTCCCCAGCACCTCCTCTTCTGCATGTAGCAGCCCTGGGCCAGAAGCAAGGG
  ATCCTGGGAGCTCAGCCTCAGTTGATCTTCCAGCCTCACCGGATTCCCCCACTCTTTCCTCAGAAGCCTC
  TGAGTCTCTTCCAAACATCCCACACACTTCACCTGAGCCACCTGAACAGATTTCCTGCCCGGGGCCCTCA
  TGGACGGTTGGATCAGGGCCGAAGTGATGACTATGACTCCAAGAAACGCAAACAGCGGGCTGGTGGAGAG
  CCCTGGGGTGCTAAGAAGCCAAGGCATGACCTGCCTCCTTACCGGGTCCACCTCACTCCTTACACTGTGG
  ACAGCCCCATCTGTGACTTCCTAGAACTCCAGCGCCGTTACCGCAGCCTCCTGGTCCCCTCAGATTTTCT
  GTCCGTGCATCTGAGTTGGCTATCAGCCTTCCCCCTGAGCCAGCCCTTTTCCCTCCATCATCCAAGCCGG
  ATCCAGGTCTCTTCTGAAAAGGAGGCAGCTCCAGACGCTGGTGCTGAGCCCATCACTGCAGACAGTGACC
  CCGCTTATAGTTCGAAGGTACTGCTGCTCTCTTCCCCGGGGTTGGAGGAATTGTATCGTTGTTGCATGCT
  CTTTGTGGATGACATGGCTGAGCCAAGGGAGACGCCAGAGCATCCTCTGAAGCAGATTAAGTTTTTGCTG
  GGCAGGAAAGAAGAGGAGGCAGTGCTGGTTGGGGGTGAATGGTCTCCTTCCCTGGATGGCCTCGACCCCC
  AGGCTGACCCGCAGGTGCTGGTGCGTACCGCCATCCGCTGTGCGCAGGCCCAGACTGGCATTGATTTGAG
  CGGCTGTACCAAGTGGTGGCGCTTTGCCGAGTTTCAGTACCTGCAGCCGGGACCCCCCCGGCGGCTTCAG
  ACAGTGGTGGTGTACCTGCCGGATGTCTGGACCATCATGCCTACTTTGGAGGAGTGGGAGGCCCTGTGCC
  AGCAGAAAGCTGCAGAGGCAGCTCCCCCAACCCAGGAGGCACAAGGGGAAACGGAGCCTACTGAACAGGC
  ACCTGATGCCTTGGAGCAAGCAGCAGACACTTCTAGACGGAACGCAGAAACTCCAGAGGCCACCACACAG
  CAGGAAACGGACACTGATCTCCCAGAGGCCCCTCCACCCCCCCTAGAACCTGCTGTCATCGCACGCCCTG
  GCTGTGTAAACCTGTCCCTCCATGGGATTGTGGAGGATCGGAGGCCAAAGGAAAGGATCTCTTTTGAGGT
  GATGGTGCTGGCCGAGCTGTTTCTGGAGATGCTCCAGAGGGATTTTGGCTATAGAGTTTATAAGATGCTA
  CTGAGCCTTCCTGAAAAGGTCGTGTCCCCACCTGAACCTGAGAAGGAGGAGGCGGCCAAGGAAGAAGCCA
  CCAAGGAGGAAGAAGCCATCAAAGAGGAGGTGGTCAAGGAGCCCAAGGATGAGGCACAGAATGAGGGCCC
  GGCTACAGAGTCAGAGGCCCCGCTGAAGGAGGATGGGCTTTTGCCCAAACCACTCTCTTCTGGGGGAGAG
  GAAGAAGAAAAACCCCGGGGCGAGGCTTCTGAGGACCTGTGTGAGATGGCCCTGGACCCAGAACTGTTGC
  TTCTGAGGGATGATGGAGAGGAGGAGTTTGCAGGAGCAAAGCTGGAGGATTCGGAGGTCCGGTCCGTTGC
  CTCAAACCAGTCAGAGATGGAGTTCTCTTCACTTCAGGACATGCCCAAGGAGCTGGATCCCTCTGCTGTG
  CTCCCCTTAGACTGTCTGCTTGCTTTTGTGTTCTTTGATGCCAACTGGTGTGGCTACTTGCACCGGCGAG
  ACTTAGAGAGGATCCTCCTTACCCTTGGGATCCGGCTCAGTGCAGAGCAGGCCAAGCAGCTGGTCAGCAG
  GGTGGTGACCCAGAACATCTGCCAGTACCGGAGCCTTCAGTACAGCCGCCAGGAGGGCCTGGATGGTGGC
  CTTCCCGAGGAGGTGCTCTTCGGAAACCTGGACCTGCTGCCCCCTCCTGGGAAAAGCACGAAGCCAGGTG
  CTGCCCCCACAGAACACAAAGCCTTGGTGTCCCACAATGGCAGCCTGATTAACGTGGGGAGCCTGCTGCA
  GCGCGCGGAGCAGCAGGACAGCGGCCGGCTCTACCTAGAGAACAAGATCCACACACTGGAGCTGAAGCTG
  GAGGAGAGCCATAACCGTTTCTCAGCCACTGAAGTAACCAATAAGACGCTGGCGGCAGAGATGCAGGAGC
  TGCGAGTCCGGCTGGCGGAGGCCGAGGAGACCGCCCGGACGGCGGAGCGACAGAAGAGCCAGCTCCAGCG
  GCTGCTGCAGGAGCTCCGCAGGCGTCTGACCCCCCTGCAGCTGGAGATCCAGCGGGTGGTGGAAAAGGCT
  GACAGCTGGGTGGAGAAGGAGGAGCCGGCACCTAGCAAC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220900 representing NM_021174
  Red=Cloning site Green=Tags(s)
  
  MSQFKRQRINPLPGGRNFSGTASTSLLGPPPGLLTPPVATELSQNARHLQGGEKQRVFTGIVTSLHDYFG
  VVDEEVFFQLSVVKGRLPQLGEKVLVKAAYNPGQAVPWNAVKVQTLSNQPLLKSPAPPLLHVAALGQKQG
  ILGAQPQLIFQPHRIPPLFPQKPLSLFQTSHTLHLSHLNRFPARGPHGRLDQGRSDDYDSKKRKQRAGGE
  PWGAKKPRHDLPPYRVHLTPYTVDSPICDFLELQRRYRSLLVPSDFLSVHLSWLSAFPLSQPFSLHHPSR
  IQVSSEKEAAPDAGAEPITADSDPAYSSKVLLLSSPGLEELYRCCMLFVDDMAEPRETPEHPLKQIKFLL
  GRKEEEAVLVGGEWSPSLDGLDPQADPQVLVRTAIRCAQAQTGIDLSGCTKWWRFAEFQYLQPGPPRRLQ
  TVVVYLPDVWTIMPTLEEWEALCQQKAAEAAPPTQEAQGETEPTEQAPDALEQAADTSRRNAETPEATTQ
  QETDTDLPEAPPPPLEPAVIARPGCVNLSLHGIVEDRRPKERISFEVMVLAELFLEMLQRDFGYRVYKML
  LSLPEKVVSPPEPEKEEAAKEEATKEEEAIKEEVVKEPKDEAQNEGPATESEAPLKEDGLLPKPLSSGGE
  EEEKPRGEASEDLCEMALDPELLLLRDDGEEEFAGAKLEDSEVRSVASNQSEMEFSSLQDMPKELDPSAV
  LPLDCLLAFVFFDANWCGYLHRRDLERILLTLGIRLSAEQAKQLVSRVVTQNICQYRSLQYSRQEGLDGG
  LPEEVLFGNLDLLPPPGKSTKPGAAPTEHKALVSHNGSLINVGSLLQRAEQQDSGRLYLENKIHTLELKL
  EESHNRFSATEVTNKTLAAEMQELRVRLAEAEETARTAERQKSQLQRLLQELRRRLTPLQLEIQRVVEKA
  DSWVEKEEPAPSN
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_021174
• ORF Size: 2769 bp
• OTI Disclaimer: Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery.
  
  The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_021174.3, NM_021174.4, NM_021174.5, NP_066997.3
• RefSeq Size: 4031
• RefSeq ORF: 2772
• Locus ID: 57805
• MW: 102.7 kDa