RNF98 (TRIM36) (NM_018700) Human Tagged ORF Clone

CAT#: RC220915

TRIM36 (Myc-DDK-tagged)-Human tripartite motif containing 36 (TRIM36), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY412895) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220915 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: TRIM36
• Synonyms: ANPH; HAPRIN; RBCC728; RNF98
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220915 representing NM_018700
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGTCGGAGTCTGGGGAGATGAGTGAATTTGGCTACATCATGGAATTGATAGCTAAAGGCAAGGTTACCA
  TTAAGAATATCGAAAGGGAGCTCATTTGCCCAGCATGCAAGGAGCTGTTTACCCACCCATTGATTCTCCC
  TTGCCAACATAGTATCTGTCATAAATGTGTAAAAGAACTCCTGCTGACTCTCGATGATTCATTCAACGAT
  GTGGGATCAGACAACTCCAATCAAAGCAGTCCTCGACTTCGGCTCCCCTCCCCTAGTATGGATAAAATTG
  ACCGAATTAACAGACCAGGCTGGAAGCGCAATTCATTGACCCCGAGGACAACTGTTTTCCCTTGCCCTGG
  CTGTGAGCATGATGTGGATCTTGGAGAACGAGGAATCAATGGTCTGTTTCGAAACTTCACTTTGGAAACT
  ATTGTGGAAAGATATCGTCAAGCAGCTAGGGCAGCCACAGCCATTATGTGTGACCTTTGTAAACCACCAC
  CTCAAGAATCCACAAAAAGCTGCATGGACTGTAGTGCAAGTTACTGCAATGAATGCTTCAAAATTCATCA
  CCCTTGGGGTACTATAAAAGCTCAACATGAGTATGTTGGTCCAACTACTAACTTCAGACCCAAGATTTTA
  ATGTGCCCAGAACATGAAACAGAGAGAATAAACATGTACTGTGAATTATGTAGGAGGCCAGTTTGCCATC
  TGTGTAAGTTGGGTGGTAATCATGCCAACCACCGTGTAACCACTATGAGCAGTGCCTACAAAACCTTAAA
  GGAAAAGCTTTCAAAGGATATTGATTACCTTATTGGTAAGGAAAGCCAGGTGAAGAGTCAAATATCTGAA
  CTAAACTTGTTAATGAAAGAAACAGAGTGTAATGGAGAGAGGGCTAAAGAAGAAGCAATTACACATTTTG
  AAAAGCTCTTTGAAGTTCTGGAAGAGAGGAAATCATCTGTTTTGAAAGCAATTGACTCCTCTAAGAAACT
  AAGATTAGACAAATTTCAGACTCAAATGGAAGAGTACCAGGGACTTCTAGAGAACAATGGACTTGTGGGA
  TATGCTCAAGAAGTGCTAAAGGAGACAGATCAGTCTTGCTTTGTGCAGACAGCAAAGCAGCTCCACCTCA
  GAATACAGAAAGCCACAGAATCTTTGAAGAGCTTTAGACCTGCAGCTCAGACTTCTTTTGAAGACTATGT
  TGTTAATACCTCTAAACAAACAGAACTTCTTGGAGAATTATCCTTTTTCTCTAGTGGCATAGACGTGCCA
  GAGATCAATGAGGAACAGAGCAGAGTTTATAACAATGCCTTGATAAATTGGCACCATCCAGAAAAGGATA
  AAGCTGATAGCTATGTTCTTGAATATCGGAAAATCAATAGAGATGATGAAATGTCATGGAATGAGATAGA
  AGTGTGTGGAACAAGTAAAATAATTCAAGACTTGGAAAACAGTAGTACCTATGCTTTCAGAGTAAGAGCT
  TACAAGGGTTCAATCTGTAGTCCTTGCAGCAGAGAATTGATTCTTCATACTCCTCCAGCTCCAGTTTTCA
  GCTTCCTCTTTGATGAAAAATGTGGCTATAATAATGAACACCTCCTGCTGAACTTGAAGAGAGACCGTGT
  AGAGAGTAGAGCTGGATTTAATCTTCTGCTTGCTGCAGAACGCATCCAAGTGGGTTATTACACAAGCTTA
  GACTACATCATTGGAGATACTGGCATTACAAAAGGAAAACACTTCTGGGCCTTCCGTGTGGAACCATATT
  CATACCTGGTAAAAGTGGGAGTTGCTTCTAGCGATAAACTACAAGAATGGCTCCGTTCTCCCCGGGATGC
  AGTTAGTCCAAGATATGAGCAAGACAGTGGGCATGACAGTGGAAGTGAGGATGCCTGTTTTGATTCTTCA
  CAACCATTTACCTTAGTTACTATAGGCATGCAGAAATTTTTTATACCCAAGTCACCTACTTCTTCTAATG
  AACCTGAAAATAGAGTTCTCCCTATGCCAACAAGTATTGGGATTTTCCTTGACTGTGATAAAGGCAAAGT
  AAATTTCTATGATATGGATCAGATGAAATGCCTTTATGAACGCCAAGTGGACTGTTCACATACACTGTAT
  CCAGCATTTGCATTAATGGGCAGTGGAGGAATTCAGCTTGAAGAACCCATCACAGCAAAATATCTGGAAT
  ACCAAGAGGACATG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220915 representing NM_018700
  Red=Cloning site Green=Tags(s)
  
  MSESGEMSEFGYIMELIAKGKVTIKNIERELICPACKELFTHPLILPCQHSICHKCVKELLLTLDDSFND
  VGSDNSNQSSPRLRLPSPSMDKIDRINRPGWKRNSLTPRTTVFPCPGCEHDVDLGERGINGLFRNFTLET
  IVERYRQAARAATAIMCDLCKPPPQESTKSCMDCSASYCNECFKIHHPWGTIKAQHEYVGPTTNFRPKIL
  MCPEHETERINMYCELCRRPVCHLCKLGGNHANHRVTTMSSAYKTLKEKLSKDIDYLIGKESQVKSQISE
  LNLLMKETECNGERAKEEAITHFEKLFEVLEERKSSVLKAIDSSKKLRLDKFQTQMEEYQGLLENNGLVG
  YAQEVLKETDQSCFVQTAKQLHLRIQKATESLKSFRPAAQTSFEDYVVNTSKQTELLGELSFFSSGIDVP
  EINEEQSRVYNNALINWHHPEKDKADSYVLEYRKINRDDEMSWNEIEVCGTSKIIQDLENSSTYAFRVRA
  YKGSICSPCSRELILHTPPAPVFSFLFDEKCGYNNEHLLLNLKRDRVESRAGFNLLLAAERIQVGYYTSL
  DYIIGDTGITKGKHFWAFRVEPYSYLVKVGVASSDKLQEWLRSPRDAVSPRYEQDSGHDSGSEDACFDSS
  QPFTLVTIGMQKFFIPKSPTSSNEPENRVLPMPTSIGIFLDCDKGKVNFYDMDQMKCLYERQVDCSHTLY
  PAFALMGSGGIQLEEPITAKYLEYQEDM
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_018700
• ORF Size: 2184 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_018700.1, NM_018700.2, NM_018700.3, NP_061170.2
• RefSeq Size: 4456
• RefSeq ORF: 2187
• Locus ID: 55521
• Domains: zf-B_box, SPRY, FN3
• Protein Families: Druggable Genome
• MW: 82.8 kDa
• Gene Summary: The protein encoded by this gene is a member of the tripartite motif (TRIM) family. The TRIM motif includes three zinc-binding domains, a RING, a B-box type 1 and a B-box type 2, and a coiled-coil region. Multiple alternatively spliced transcript variants that encode different protein isoforms have been described for this gene. [provided by RefSeq, Jul 2008]