OSBPL9 (NM_148905) Human Tagged ORF Clone

CAT#: RC220953

OSBPL9 (Myc-DDK-tagged)-Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 2

Product Images

Western blot validation of overexpression lysate (Cat# LY407761) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC220953 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OSBPL9
• Synonyms: ORP-9; ORP9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC220953 representing NM_148905
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGTAGAATCAATTAAACACTGCATTGTGTTGCTGCAGATTGCCAAAAGTACTATTAATCCCGTAGATG
  CAATATATCAACCTAGTCCTTTGGAACCTGTGATCAGCACAATGCCTTCCCAGACTGTGTTACCTCCAGA
  ACCTGTTCAGTTGTGTAAGTCAGAGCAGCGTCCATCTTCCCTACCAGTTGGACCTGTGTTGGCTACCTTG
  GGACATCATCAGACTCCTACACCAAATAGTACAGGCAGTGGCCATTCACCACCGAGTAGCAGTCTCACTT
  CTCCAAGCCACGTGAACTTGTCTCCAAATACAGTCCCAGAGTTCTCTTACTCCAGCAGTGAAGATGAATT
  TTATGATGCTGATGAATTCCATCAAAGTGGCTCATCCCCAAAGCGCTTAATAGATTCTTCTGGATCTGCC
  TCAGTCCTGACACACAGCAGCTCGGGAAATAGTCTAAAACGCCCAGATACCACAGAATCACTTAATTCTT
  CCTTGTCCAATGGAACAAGTGATGCTGACCTGTTTGATTCACATGATGACAGAGATGATGATGCGGAGGC
  AGGGTCTGTGGAGGAGCACAAGAGCGTTATCATGCATCTCTTGTCGCAGGTTAGACTTGGAATGGATCTT
  ACTAAGGTAGTTCTTCCAACGTTTATTCTTGAAAGAAGATCTCTTTTAGAAATGTATGCAGACTTTTTTG
  CACATCCGGACCTGTTTGTGAGCATTAGTGACCAGAAGGATCCCAAGGATCGAATGGTTCAGGTTGTGAA
  ATGGTACCTCTCAGCCTTTCATGCGGGAAGGAAAGGATCAGTTGCCAAAAAGCCATACAATCCCATTTTG
  GGCGAGATTTTTCAGTGTCATTGGACATTACCAAATGATACTGAAGAGAACACAGAACTAGTTTCAGAAG
  GACCAGTTCCCTGGGTTTCCAAAAACAGTGTAACATTTGTGGCTGAGCAGGTTTCCCATCATCCACCCAT
  TTCAGCCTTTTATGCTGAGTGTTTTAACAAGAAGATACAATTCAATGCTCATATCTGGACCAAATCAAAA
  TTCCTTGGGATGTCAATTGGGGTGCACAACATAGGGCAGGGCTGTGTCTCATGTCTAGACTATGATGAAC
  ATTACATTCTCACATTCCCCAATGGCTATGGAAGGTCTATCCTCACAGTGCCCTGGGTGGAATTAGGAGG
  AGAATGCAATATTAATTGTTCCAAAACAGGCTATAGTGCAAATATCATCTTCCACACTAAACCCTTCTAT
  GGGGGCAAGAAGCACAGAATTACTGCCGAGATTTTTTCTCCAAATGACAAGAAGTCTTTTTGCTCAATTG
  AAGGGGAATGGAATGGTGTGATGTATGCAAAATATGCAACAGGGGAAAATACAGTCTTTGTAGATACCAA
  GAAGTTGCCTATAATCAAGAAGAAAGTGAGGAAGTTGGAAGATCAGAACGAGTATGAATCCCGCAGCCTT
  TGGAAGGATGTCACTTTCAACTTAAAAATCAGAGACATTGATGCAGCAACTGAAGCAAAGCACAGGCTTG
  AAGAAAGACAAAGAGCAGAAGCCCGAGAAAGGAAGGAGAAGGAAATTCAGTGGGAGACAAGGTTATTTCA
  TGAAGATGGAGAATGCTGGGTTTATGATGAACCATTACTGAAACGTCTTGGTGCTGCCAAGCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC220953 representing NM_148905
  Red=Cloning site Green=Tags(s)
  
  MVESIKHCIVLLQIAKSTINPVDAIYQPSPLEPVISTMPSQTVLPPEPVQLCKSEQRPSSLPVGPVLATL
  GHHQTPTPNSTGSGHSPPSSSLTSPSHVNLSPNTVPEFSYSSSEDEFYDADEFHQSGSSPKRLIDSSGSA
  SVLTHSSSGNSLKRPDTTESLNSSLSNGTSDADLFDSHDDRDDDAEAGSVEEHKSVIMHLLSQVRLGMDL
  TKVVLPTFILERRSLLEMYADFFAHPDLFVSISDQKDPKDRMVQVVKWYLSAFHAGRKGSVAKKPYNPIL
  GEIFQCHWTLPNDTEENTELVSEGPVPWVSKNSVTFVAEQVSHHPPISAFYAECFNKKIQFNAHIWTKSK
  FLGMSIGVHNIGQGCVSCLDYDEHYILTFPNGYGRSILTVPWVELGGECNINCSKTGYSANIIFHTKPFY
  GGKKHRITAEIFSPNDKKSFCSIEGEWNGVMYAKYATGENTVFVDTKKLPIIKKKVRKLEDQNEYESRSL
  WKDVTFNLKIRDIDAATEAKHRLEERQRAEARERKEKEIQWETRLFHEDGECWVYDEPLLKRLGAAKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_148905
• ORF Size: 1674 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_148905.1, NM_148905.2, NM_148905.3, NP_683703.1
• RefSeq Size: 2751 bp
• RefSeq ORF: 1677 bp
• Locus ID: 114883
• MW: 62.3 kDa
• Gene Summary: This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. This family member functions as a cholesterol transfer protein that regulates Golgi structure and function. Multiple transcript variants, most of which encode distinct isoforms, have been identified. Related pseudogenes have been identified on chromosomes 3, 11 and 12. [provided by RefSeq, Jul 2010]