MTF2 (NM_007358) Human Tagged ORF Clone

CAT#: RC221006

MTF2 (Myc-DDK-tagged)-Human metal response element binding transcription factor 2 (MTF2), transcript variant 1

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: MTF2
• Synonyms: dJ976O13.2; M96; PCL2; TDRD19A
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221006 representing NM_007358
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGAGAGACTCTACAGGGGCAGGTAATTCACTGGTCCACAAGCGGTCTCCTTTACGTCGAAACCAAAAGA
  CCCCAACATCCTTGACCAAGCTGTCTTTACAGGATGGACATAAAGCCAAAAAGCCAGCATGTAAATTTGA
  AGAGGGTCAGGATGTCCTAGCTAGATGGTCAGATGGCTTGTTTTATCTTGGCACTATCAAAAAGATAAAC
  ATATTGAAACAGAGCTGCTTCATCATATTTGAAGACAGTTCTAAATCCTGGGTTCTCTGGAAGGACATTC
  AAACAGGAGCCACTGGAAGTGGGGAAATGGTCTGTACAATATGTCAAGAAGAGTATTCAGAAGCTCCCAA
  TGAAATGGTTATATGTGACAAGTGTGGCCAAGGATATCATCAGTTGTGTCACACACCTCATATTGATTCC
  AGTGTGATTGATTCAGATGAAAAATGGCTCTGTCGGCAGTGTGTTTTTGCAACAACAACAAAGAGGGGTG
  GTGCACTTAAGAAAGGACCAAATGCCAAAGCATTGCAAGTCATGAAGCAGACATTACCCTATAGTGTGGC
  AGACCTTGAATGGGATGCAGGTCATAAAACCAATGTCCAGCAGTGTTACTGCTATTGTGGAGGCCCTGGA
  GACTGGTATTTGAAGATGCTACAGTGCTGCAAATGTAAGCAGTGGTTTCATGAGGCTTGTGTGCAATGCC
  TTCAAAAGCCAATGCTATTTGGAGACAGATTTTATACGTTTATATGCTCTGTCTGCAGTTCTGGACCAGA
  ATACCTCAAACGTCTACCATTACAGTGGGTAGATATAGCACACCTATGCCTTTACAACCTAAGTGTTATT
  CATAAGAAGAAATACTTTGATTCTGAACTTGAGCTTATGACATACATTAATGAAAACTGGGATAGATTGC
  ACCCTGGAGAGCTGGCAGACACACCAAAATCTGAAAGATATGAGCATGTTCTGGAGGCATTAAATGATTA
  CAAGACCATGTTTATGTCTGGGAAAGAAATAAAGAAGAAGAAGCATTTGTTTGGGTTGCGAATTCGTGTT
  CCTCCTGTGCCACCAAATGTGGCTTTCAAAGCAGAGAAAGAACCTGAAGGAACATCTCATGAATTTAAAA
  TTAAAGGCAGAAAGGCATCCAAACCTATATCTGATTCAAGGGAAGTAAGCAATGGCATAGAAAAAAAAGG
  AAAGAAAAAATCTGTAGGTCGTCCACCTGGCCCATATACAAGAAAAATGATTCAAAAAACTGCTGAGCCA
  CTTTTGGATAAGGAATCAATTTCAGAGAATCCTACTTTGGATTTACCTTGTTCTATAGGGAGAACTGAGG
  GAACTGCACATTCATCCAATACCTCAGATGTGGATTTCACGGGTGCTTCCAGTGCAAAAGAAACTACCTC
  GTCTAGCATTTCCAGGCATTATGGATTATCTGACTCCAGAAAAAGAACGCGTACAGGAAGATCTTGGCCT
  GCTGCAATACCACATTTGCGGAGAAGAAGAGGTCGTCTTCCAAGAAGAGCACTCCAGACTCAGAACTCAG
  AAATTGTAAAAGATGATGAAGGCAAAGAAGATTATCAGTTTGATGAACTCAACACAGAGATTCTGAATAA
  CTTAGCAGATCAGGAGTTACAACTCAATCATCTAAAGAACTCCATTACCAGTTATTTTGGTGCTGCAGGT
  AGAATAGCATGTGGCGAAAAATACCGAGTTTTGGCACGTCGGGTGACACTTGATGGAAAGGTGCAGTATC
  TTGTGGAATGGGAAGGAGCAACTGCATCCAGCGGACCG
  
  AGCGGACCGACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCC
  TGGATTACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221006 representing NM_007358
  Red=Cloning site Green=Tags(s)
  
  MRDSTGAGNSLVHKRSPLRRNQKTPTSLTKLSLQDGHKAKKPACKFEEGQDVLARWSDGLFYLGTIKKIN
  ILKQSCFIIFEDSSKSWVLWKDIQTGATGSGEMVCTICQEEYSEAPNEMVICDKCGQGYHQLCHTPHIDS
  SVIDSDEKWLCRQCVFATTTKRGGALKKGPNAKALQVMKQTLPYSVADLEWDAGHKTNVQQCYCYCGGPG
  DWYLKMLQCCKCKQWFHEACVQCLQKPMLFGDRFYTFICSVCSSGPEYLKRLPLQWVDIAHLCLYNLSVI
  HKKKYFDSELELMTYINENWDRLHPGELADTPKSERYEHVLEALNDYKTMFMSGKEIKKKKHLFGLRIRV
  PPVPPNVAFKAEKEPEGTSHEFKIKGRKASKPISDSREVSNGIEKKGKKKSVGRPPGPYTRKMIQKTAEP
  LLDKESISENPTLDLPCSIGRTEGTAHSSNTSDVDFTGASSAKETTSSSISRHYGLSDSRKRTRTGRSWP
  AAIPHLRRRRGRLPRRALQTQNSEIVKDDEGKEDYQFDELNTEILNNLADQELQLNHLKNSITSYFGAAG
  RIACGEKYRVLARRVTLDGKVQYLVEWEGATASSGP
  
  SGPTRTRRLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-RsrII Cloning Scheme for this gene Plasmid Map
• ACCN: NM_007358
• ORF Size: 2174 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_007358.1, NM_007358.2, NM_007358.3, NP_031384.1
• RefSeq Size: 2648 bp
• RefSeq ORF: 1782 bp
• Locus ID: 22823
• Domains: PHD, TUDOR
• Protein Families: Druggable Genome, Transcription Factors
• MW: 66.9 kDa