OSBPL9 (NM_148908) Human Tagged ORF Clone

CAT#: RC221053

OSBPL9 (Myc-DDK-tagged)-Human oxysterol binding protein-like 9 (OSBPL9), transcript variant 5

Product Images

Western blot validation of overexpression lysate (Cat# LY407764) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221053 using transfection reagent MegaTran 2.0 (Cat# TT210002).

• 

  Coomassie blue staining of purified OSBPL9 protein (Cat# TP321053). The protein was produced from HEK293T cells transfected with OSBPL9 cDNA clone (Cat# RC221053) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OSBPL9
• Synonyms: ORP-9; ORP9
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221053 representing NM_148908
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGTCCATCATGGAAGGGCCGCTGAGCAAATGGACTAACGTGATGAAGGGCTGGCAGTACCGTTGGT
  TCGTGCTGGACTACAATGCAGGACTGCTCTCCTACTACACGTCCAAGGACAAAATGATGAGAGGCTCTCG
  CAGAGGATGTGTTAGACTCAGAGGAGCTGTGATTGGTATAGACGATGAGGACGACAGCACCTTCACAATA
  ACTGTTGATCAGAAAACCTTCCATTTCCAGGCCCGTGATGCTGATGAGCGAGAGAAGTGGATCCATGCCT
  TAGAAGAAACAATTCTTCGACATACTCTCCAGCTTCAAGGTTTGGATTCAGGATTTGTTCCTAGTGTCCA
  AGATTTTGATAAGAAACTTACAGAAGCTGATGCTTACCTACAAATCTTGATTGAACAATTAAAGCTTTTT
  GATGACAAGCTTCAAAACTGCAAAGAAGATGAACAGAGAAAGAAAATTGAAACTCTCAAAGAGACAACAA
  ATAGCATGGTAGAATCAATTAAACACTGCATTGTGTTGCTGCAGATTGCCAAAAGTACTATTAATCCCGT
  AGATGCAATATATCAACCTAGTCCTTTGGAACCTGTGATCAGCACAATGCCTTCCCAGACTGTGTTACCT
  CCAGAACCTGTTCAGTTGTGTAAGTCAGAGCAGCGTCCATCTTCCCTACCAGTTGGACCTGTGTTGGCTA
  CCTTGGGACATCATCAGACTCCTACACCAAATAGTACAGGCAGTGGCCATTCACCACCGAGTAGCAGTCT
  CACTTCTCCAAGCCACGTGAACTTGTCTCCAAATACAGTCCCAGAGTTCTCTTACTCCAGCAGTGAAGAT
  GAATTTTATGATGCTGATGAATTCCATCAAAGTGGCTCATCCCCAAAGCGCTTAATAGATTCTTCTGGAT
  CTGCCTCAGTCCTGACACACAGCAGCTCGGGAAATAGTCTAAAACGCCCAGATACCACAGAATCACTTAA
  TTCTTCCTTGTCCAATGGAACAAGTGATGCTGACCTGTTTGATTCACATGATGACAGAGATGATGATGCG
  GAGGCAGGGTCTGTGGAGGAGCACAAGAGCGTTATCATGCATCTCTTGTCGCAGGTTAGACTTGGAATGG
  ATCTTACTAAGGTAGTTCTTCCAACGTTTATTCTTGAAAGAAGATCTCTTTTAGAAATGTATGCAGACTT
  TTTTGCACATCCGGACCTGTTTGTGAGCATTAGTGACCAGAAGGATCCCAAGGATCGAATGGTTCAGGTT
  GTGAAATGGTACCTCTCAGCCTTTCATGCGGGAAGGAAAGGATCAGTTGCCAAAAAGCCATACAATCCCA
  TTTTGGGCGAGATTTTTCAGTGTCATTGGACATTACCAAATGATACTGAAGAGAACACAGAACTAGTTTC
  AGAAGGACCAGTTCCCTGGGTTTCCAAAAACAGTGTAACATTTGTGGCTGAGCAGGTTTCCCATCATCCA
  CCCATTTCAGCCTTTTATGCTGAGTGTTTTAACAAGAAGATACAATTCAATGCTCATATCTGGACCAAAT
  CAAAATTCCTTGGGATGTCAATTGGGGTGCACAACATAGGGCAGGGCTGTGTCTCATGTCTAGACTATGA
  TGAACATTACATTCTCACATTCCCCAATGGCTATGGAAGGTCTATCCTCACAGTGCCCTGGGTGGAATTA
  GGAGGAGAATGCAATATTAATTGTTCCAAAACAGGCTATAGTGCAAATATCATCTTCCACACTAAACCCT
  TCTATGGGGGCAAGAAGCACAGAATTACTGCCGAGATTTTTTCTCCAAATGACAAGAAGTCTTTTTGCTC
  AATTGAAGGGGAATGGAATGGTGTGATGTATGCAAAATATGCAACAGGGGAAAATACAGTCTTTGTAGAT
  ACCAAGAAGTTGCCTATAATCAAGAAGAAAGTGAGGAAGTTGGAAGATCAGAACGAGTATGAATCCCGCA
  GCCTTTGGAAGGATGTCACTTTCAACTTAAAAATCAGAGACATTGATGCAGCAACTGAAGCAAAGCACAG
  GCTTGAAGAAAGACAAAGAGCAGAAGCCCGAGAAAGGAAGGAGAAGGAAATTCAGTGGGAGACAAGGTTA
  TTTCATGAAGATGGAGAATGCTGGGTTTATGATGAACCATTACTGAAACGTCTTGGTGCTGCCAAGCAT
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221053 representing NM_148908
  Red=Cloning site Green=Tags(s)
  
  MASIMEGPLSKWTNVMKGWQYRWFVLDYNAGLLSYYTSKDKMMRGSRRGCVRLRGAVIGIDDEDDSTFTI
  TVDQKTFHFQARDADEREKWIHALEETILRHTLQLQGLDSGFVPSVQDFDKKLTEADAYLQILIEQLKLF
  DDKLQNCKEDEQRKKIETLKETTNSMVESIKHCIVLLQIAKSTINPVDAIYQPSPLEPVISTMPSQTVLP
  PEPVQLCKSEQRPSSLPVGPVLATLGHHQTPTPNSTGSGHSPPSSSLTSPSHVNLSPNTVPEFSYSSSED
  EFYDADEFHQSGSSPKRLIDSSGSASVLTHSSSGNSLKRPDTTESLNSSLSNGTSDADLFDSHDDRDDDA
  EAGSVEEHKSVIMHLLSQVRLGMDLTKVVLPTFILERRSLLEMYADFFAHPDLFVSISDQKDPKDRMVQV
  VKWYLSAFHAGRKGSVAKKPYNPILGEIFQCHWTLPNDTEENTELVSEGPVPWVSKNSVTFVAEQVSHHP
  PISAFYAECFNKKIQFNAHIWTKSKFLGMSIGVHNIGQGCVSCLDYDEHYILTFPNGYGRSILTVPWVEL
  GGECNINCSKTGYSANIIFHTKPFYGGKKHRITAEIFSPNDKKSFCSIEGEWNGVMYAKYATGENTVFVD
  TKKLPIIKKKVRKLEDQNEYESRSLWKDVTFNLKIRDIDAATEAKHRLEERQRAEARERKEKEIQWETRL
  FHEDGECWVYDEPLLKRLGAAKH
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_148908
• ORF Size: 2169 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_148908.1, NM_148908.3, NP_683706.1, NP_683706.3
• RefSeq Size: 2880
• RefSeq ORF: 2172
• Locus ID: 114883
• MW: 81.6 kDa
• Gene Summary: This gene encodes a member of the oxysterol-binding protein (OSBP) family, a group of intracellular lipid receptors. Most members contain an N-terminal pleckstrin homology domain and a highly conserved C-terminal OSBP-like sterol-binding domain, although some members contain only the sterol-binding domain. This family member functions as a cholesterol transfer protein that regulates Golgi structure and function. Multiple transcript variants, most of which encode distinct isoforms, have been identified. Related pseudogenes have been identified on chromosomes 3, 11 and 12. [provided by RefSeq, Jul 2010]