OR5F1 (NM_003697) Human Tagged ORF Clone

CAT#: RC221057

OR5F1 (Myc-DDK-tagged)-Human olfactory receptor, family 5, subfamily F, member 1 (OR5F1)

Product Images

Western blot validation of overexpression lysate (Cat# LY418494) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221057 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: OR5F1
• Synonyms: OR11-10
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221057 representing NM_003697
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGACCAGAAAAAATTATACCTCACTGACTGAGTTCGTCCTATTGGGATTAGCAGACACGCTGGAGCTAC
  AGATTATCCTCTTTTTGTTTTTTCTTGTGATTTATACACTTACAGTACTGGGAAATCTCGGGATGATCCT
  CTTAATCAGGATCGATTCCCAGCTTCACACACCCATGTATTTCTTCCTGGCTAACCTGTCCTTTGTGGAC
  GTTTGTAACTCAACTACCATCACCCCAAAGATGCTGGCAGATTTATTATCAGAGAAGAAAACCATCTCTT
  TTGCTGGCTGCTTCCTACAGATGTACTTCTTTATCTCCCTGGCGACAACCGAATGCATCCTCTTTGGGTT
  AATGGCCTATGACCGGTATGCGGCCATATGTCGCCCGCTGCTTTACTCCTTGATCATGTCCAGGACCGTC
  TACCTAAAAATGGCAGCCGGGGCTTTTGCTGCAGGGTTGCTGAACTTCATGGTCAACACAAGCCATGTCA
  GCAGCTTGTCATTCTGTGACTCCAATGTCATCCATCACTTCTTCTGTGACAGTCCCCCACTTTTCAAGCT
  CTCTTGTTCTGACACAATCCTGAAAGAAAGCATAAGTTCTATTTTGGCTGGTGTGAATATTGTGGGGACT
  CTGCTTGTCATCCTCTCCTCCTACTCCTACGTTCTCTTCTCCATTTTTTCTATGCATTCGGGGGAGGGGA
  GGCACAGAGCTTTCTCCACGTGTGCCTCTCACCTGACAGCCATAATTCTGTTCTATGCCACCTGCATCTA
  TACTTACCTGAGACCTAGTTCCAGCTACTCCCTGAATCAGGACAAAGTGGCTTCTGTGTTCTACACAGTG
  GTGATTCCCATGTTGAATCCTCTGATCTACAGCCTCAGGAATAAGGAAGTAAAGAAGGCTTTAGCGAATG
  TAATTAGCAGGAAAAGGACCTCTTCCTTTCTG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221057 representing NM_003697
  Red=Cloning site Green=Tags(s)
  
  MTRKNYTSLTEFVLLGLADTLELQIILFLFFLVIYTLTVLGNLGMILLIRIDSQLHTPMYFFLANLSFVD
  VCNSTTITPKMLADLLSEKKTISFAGCFLQMYFFISLATTECILFGLMAYDRYAAICRPLLYSLIMSRTV
  YLKMAAGAFAAGLLNFMVNTSHVSSLSFCDSNVIHHFFCDSPPLFKLSCSDTILKESISSILAGVNIVGT
  LLVILSSYSYVLFSIFSMHSGEGRHRAFSTCASHLTAIILFYATCIYTYLRPSSSYSLNQDKVASVFYTV
  VIPMLNPLIYSLRNKEVKKALANVISRKRTSSFL
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_003697
• ORF Size: 942 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_003697.1, NP_003688.1
• RefSeq Size: 945 bp
• RefSeq ORF: 945 bp
• Locus ID: 338674
• Protein Families: Transmembrane
• Protein Pathways: Olfactory transduction
• MW: 35 kDa
• Gene Summary: Olfactory receptors interact with odorant molecules in the nose, to initiate a neuronal response that triggers the perception of a smell. The olfactory receptor proteins are members of a large family of G-protein-coupled receptors (GPCR) arising from single coding-exon genes. Olfactory receptors share a 7-transmembrane domain structure with many neurotransmitter and hormone receptors and are responsible for the recognition and G protein-mediated transduction of odorant signals. The olfactory receptor gene family is the largest in the genome. The nomenclature assigned to the olfactory receptor genes and proteins for this organism is independent of other organisms. [provided by RefSeq, Jul 2008]