XRN1 (NM_019001) Human Tagged ORF Clone

CAT#: RC221062

XRN1 (Myc-DDK-tagged)-Human 5'-3' exoribonuclease 1 (XRN1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY412839) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221062 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: XRN1
• Synonyms: SEP1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221062 representing NM_019001
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGGAGTCCCCAAGTTTTACAGATGGATCTCAGAGCGGTATCCCTGTCTCAGCGAAGTGGTGAAAGAGC
  ATCAGATTCCTGAATTTGACAACTTGTACCTGGATATGAATGGAATTATACATCAGTGCTCCCATCCTAA
  TGATGATGATGTTCACTTTAGAATTTCAGATGATAAAATCTTTACTGATATTTTTCACTACCTGGAGGTG
  TTGTTTCGCATTATTAAACCCAGGAAAGTGTTCTTTATGGCTGTAGATGGTGTGGCTCCTCGAGCAAAAA
  TGAACCAGCAGCGTGGGAGGCGTTTTAGGTCAGCAAAGGAGGCAGAAGACAAAATTAAAAAGGCAATAGA
  GAAGGGAGAAACTCTTCCTACAGAGGCCAGATTTGATTCCAACTGTATCACACCAGGAACTGAATTTATG
  GCCAGGTTACATGAACATCTGAAGTATTTTGTAAATATGAAAATTTCCACAGACAAGTCATGGCAAGGAG
  TTACCATCTACTTCTCAGGCCATGAGACTCCTGGAGAAGGAGAGCATAAAATCATGGAATTTATCAGATC
  CGAGAAAGCAAAGCCAGATCATGATCCAAACACCAGACACTGTCTTTATGGTTTAGATGCTGACTTGATT
  ATGCTTGGATTAACAAGTCATGAGGCACATTTTTCTCTCTTAAGAGAAGAAGTTCGATTTGGTGGCAAAA
  AAACACAACGGGTATGTGCTCCAGAAGAAACTACATTTCACCTTCTACACTTGTCTTTAATGAGAGAGTA
  TATTGACTATGAGTTTTCAGTATTAAAAGAAAAGATCACATTTAAATATGATATTGAAAGGATAATAGAT
  GATTGGATTTTGATGGGGTTTCTTGTTGGTAATGATTTTATCCCTCATCTACCTCATTTACATATTAATC
  ATGATGCACTGCCTCTTCTTTATGGAACATATGTTACCATCCTGCCAGAACTTGGGGGTTATATTAATGA
  AAGTGGGCACCTCAACTTACCTCGATTTGAGAAATACCTTGTGAAACTATCAGATTTTGATCGGGAGCAC
  TTCAGTGAAGTTTTTGTGGACCTAAAATGGTTTGAAAGCAAAGTTGGTAACAAGTACCTCAATGAAGCAG
  CAGGTGTCGCAGCAGAAGAAGCCAGGAACTACAAGGAAAAGAAAAAGTTAAAGGGCCAGGAAAATTCTCT
  GTGTTGGACTGCTTTAGACAAAAATGAAGGCGAAATGATAACTTCTAAGGATAATTTAGAAGATGAGACT
  GAAGATGATGACCTATTTGAAACTGAGTTTAGACAATATAAAAGAACATATTACATGACGAAGATGGGGG
  TTGACGTAGTATCTGATGACTTTCTGGCTGATCAAGCTGCATGTTATGTTCAGGCAATACAGTGGATTTT
  GCACTATTACTATCATGGAGTTCAGTCCTGGAGCTGGTATTATCCTTATCATTATGCACCTTTCCTGTCT
  GATATACACAACATCAGTACACTCAAAATCCATTTTGAACTAGGAAAACCTTTTAAGCCATTTGAACAGC
  TTCTTGCTGTACTTCCAGCAGCCAGCAAAAATTTACTTCCTGCATGCTACCAGCATTTGATGACCAATGA
  AGACTCACCAATTATAGAATATTACCCACCTGATTTTAAAACTGACCTAAATGGGAAACAACAGGAATGG
  GAAGCTGTGGTGTTAATCCCTTTTATTGATGAGAAGCGATTATTGGAAGCCATGGAGACATGTAACCACT
  CCCTCAAAAAGGAAGAGAGGAAAAGAAACCAACATAGTGAGTGCCTAATGTGCTGGTATGATAGAGACAC
  AGAGTTTATCTATCCTTCTCCATGGCCAGAAAAGTTCCCTGCCATAGAACGATGTTGTACAAGGTATAAA
  ATAATATCCTTAGATGCTTGGCGTGTAGACATAAACAAAAACAAAATAACCAGAATTGACCAGAAAGCAT
  TATATTTCTGTGGATTTCCTACTCTGAAACACATCAGACACAAATTTTTTTTGAAGAAAAGTGGTGTTCA
  AGTATTCCAGCAAAGCAGTCGTGGAGAAAACATGATGTTGGAAATCTTAGTGGATGCAGAATCAGATGAA
  CTTACCGTAGAAAATGTAGCTTCATCAGTGCTTGGAAAATCTGTCTTTGTTAATTGGCCTCACCTTGAGG
  AAGCTAGAGTCGTGGCTGTATCAGATGGAGAAACTAAGTTTTACTTGGAAGAACCTCCAGGAACACAGAA
  GCTTTATTCAGGAAGAACTGCCCCACCATCTAAAGTGGTTCATCTTGGAGATAAAGAACAATCTAACTGG
  GCAAAAGAAGTACAAGGAATTTCAGAACACTACCTGAGAAGAAAAGGAATAATAATAAATGAAACATCTG
  CAGTTGTGTATGCTCAGTTACTCACAGGTCGTAAATATCAAATAAATCAAAATGGTGAAGTTCGTCTAGA
  GAAACAGTGGTCAAAACAAGTTGTTCCTTTTGTTTATCAAACTATTGTCAAGGACATCCGAGCTTTCGAC
  TCCCGTTTCTCCAATATCAAAACATTGGATGATTTGTTTCCTCTGAGAAGTATGGTCTTTATGCTGGGAA
  CTCCCTATTATGGCTGCACTGGAGAAGTTCAGGATTCAGGTGATGTGATTACAGAAGGTAGGATTCGTGT
  GATTTTCAGCATTCCATGTGAACCCAATCTTGATGCTTTAATACAGAACCAGCATAAATATTCTATAAAG
  TACAACCCAGGATATGTGTTGGCCAGTCGCCTTGGAGTGAGTGGATACCTTGTTTCAAGGTTTACAGGAA
  GTATTTTTATTGGAAGAGGATCTAGGAGAAACCCTCATGGAGACCATAAAGCAAATGTGGGTTTAAATCT
  CAAATTCAACAAGAAAAATGAGGAGGTACCTGGATATACTAAGAAAGTTGGAAGTGAATGGATGTATTCA
  TCTGCAGCAGAACAACTTCTGGCAGAGTACTTAGAGAGAGCTCCAGAACTATTTAGTTATATAGCCAAAA
  ATAGCCAAGAGGATGTGTTCTATGAAGATGACATTTGGCCTGGAGAAAATGAGAATGGTGCTGAAAAAGT
  TCAAGAAATTATTACTTGGCTAAAAGGACATCCTGTCAGTACTTTATCTCGTTCTTCTTGTGATTTACAA
  ATTCTGGATGCAGCTATTGTTGAGAAAATTGAGGAAGAAGTCGAAAAGTGCAAGCAAAGAAAGAATAATA
  AGAAGGTGCGAGTAACAGTGAAACCCCATTTGCTATACAGACCTTTAGAACAGCAACATGGAGTCATTCC
  TGATCGGGATGCAGAATTTTGTCTTTTTGACCGTGTTGTAAATGTGAGAGAAAACTTCTCAGTTCCAGTT
  GGCCTTCGAGGCACCATCATAGGAATAAAAGGAGCTAATAGAGAAGCCGATGTACTATTTGAAGTATTAT
  TTGATGAAGAATTTCCTGGAGGGTTAACAATAAGATGCTCACCTGGTAGAGGTTATCGACTGCCAACAAG
  TGCCTTGGTGAACCTTTCTCATGGGAGTCGCTCTGAAACTGGAAATCAGAAGTTGACAGCCATCGTAAAA
  CCACAACCAGCTGTACATCAACATAGCTCAAGTTCATCAGTTTCCTCTGGGCATTTGGGAGCCCTCAACC
  ATTCCCCTCAATCACTTTTTGTTCCTACTCAAGTACCTACTAAAGATGATGATGAATTCTGCAACATTTG
  GCAGTCCTTACAGGGATCTGGAAAGATGCAATACTTTCAGCCAACTATACAAGAGAAGGGTGCAGTTCTA
  CCTCAAGAAATAAGCCAAGTAAATCAACATCATAAATCTGGCTTTAATGACAACAGTGTTAAATATCAGC
  AAAGAAAACATGACCCTCACAGAAAATTTAAAGAAGAGTGTAAGAGTCCTAAAGCTGAGTGTTGGTCCCA
  AAAAATGTCCAATAAGCAGCCTAACTCTGGAATTGAGAACTTTTTAGCATCTTTGAATATCTCCAAAGAA
  AATGAAGTACAGTCATCTCATCATGGGGAGCCTCCAAGTGAAGAGCATTTGTCACCACAGTCATTTGCCA
  TGGGAACACGGATGCTTAAAGAAATTCTAAAAATTGATGGCTCTAACACTGTGGACCATAAGAATGAAAT
  CAAACAGATTGCTAATGAAATCCCTGTTTCCTCTAACAGAAGAGATGAATATGGATTACCCTCTCAGCCT
  AAACAAAATAAGAAATTAGCATCTTATATGAACAAGCCTCACAGTGCTAATGAGTACCATAATGTTCAGT
  CTATGGACAATATGTGTTGGCCTGCCCCCAGCCAGATCCCTCCTGTATCCACACCAGTAACTGAACTTTC
  TCGAATTTGTTCCCTTGTTGGAATGCCACAACCTGATTTCTCCTTTCTTAGGATGCCACAGACAATGACC
  GTTTGCCAAGTAAAATTATCTAATGGCTTACTGGTACATGGGCCACAGTGCCACTCTGAAAATGAAGCCA
  AAGAGAAAGCTGCACTTTTTGCTTTACAACAGTTGGGCTCCTTAGGCATGAATTTCCCTTTGCCTTCACA
  AGTATTTGCAAATTATCCTTCAGCTGTACCACCTGGAACCATTCCTCCAGCCTTTCCCCCACCTACTGGC
  TGGGATCACTATGGAAGCAACTATGCATTGGGGGCAGCTAATATAATGCCTTCGTCGTCTCATCTCTTTG
  GCTCAATGCCATGGGGACCATCGGTGCCAGTTCCTGGGAAGCCCTTCCATCATACTTTATATTCTGGGAC
  CATGCCCATGGCTGGGGGAATACCAGGGGGTGTGCACAATCAGTTTATACCTCTGCAGGTTACTAAAAAA
  AGGGTTGCAAACAAAAAGAACTTTGAGAATAAGGAAGCCCAGAGTTCTCAAGCCACTCCAGTTCAGACTA
  GCCAGCCAGATTCTTCCAACATTGTCAAAGTAAGTCCACGGGAGAGCTCATCAGCTTCTTTGAAGTCCTC
  TCCGATTGCTCAACCTGCATCTTCTTTTCAAGTTGAAACTGCCTCTCAAGGCCATAGTATATCTCACCAT
  AAGTCAACACCAATCTCTTCTTCAAGAAGAAAATCAAGAAAACTGGCTGTTAATTTTGGTGTTTCTAAAC
  CTTCTGAG
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221062 representing NM_019001
  Red=Cloning site Green=Tags(s)
  
  MGVPKFYRWISERYPCLSEVVKEHQIPEFDNLYLDMNGIIHQCSHPNDDDVHFRISDDKIFTDIFHYLEV
  LFRIIKPRKVFFMAVDGVAPRAKMNQQRGRRFRSAKEAEDKIKKAIEKGETLPTEARFDSNCITPGTEFM
  ARLHEHLKYFVNMKISTDKSWQGVTIYFSGHETPGEGEHKIMEFIRSEKAKPDHDPNTRHCLYGLDADLI
  MLGLTSHEAHFSLLREEVRFGGKKTQRVCAPEETTFHLLHLSLMREYIDYEFSVLKEKITFKYDIERIID
  DWILMGFLVGNDFIPHLPHLHINHDALPLLYGTYVTILPELGGYINESGHLNLPRFEKYLVKLSDFDREH
  FSEVFVDLKWFESKVGNKYLNEAAGVAAEEARNYKEKKKLKGQENSLCWTALDKNEGEMITSKDNLEDET
  EDDDLFETEFRQYKRTYYMTKMGVDVVSDDFLADQAACYVQAIQWILHYYYHGVQSWSWYYPYHYAPFLS
  DIHNISTLKIHFELGKPFKPFEQLLAVLPAASKNLLPACYQHLMTNEDSPIIEYYPPDFKTDLNGKQQEW
  EAVVLIPFIDEKRLLEAMETCNHSLKKEERKRNQHSECLMCWYDRDTEFIYPSPWPEKFPAIERCCTRYK
  IISLDAWRVDINKNKITRIDQKALYFCGFPTLKHIRHKFFLKKSGVQVFQQSSRGENMMLEILVDAESDE
  LTVENVASSVLGKSVFVNWPHLEEARVVAVSDGETKFYLEEPPGTQKLYSGRTAPPSKVVHLGDKEQSNW
  AKEVQGISEHYLRRKGIIINETSAVVYAQLLTGRKYQINQNGEVRLEKQWSKQVVPFVYQTIVKDIRAFD
  SRFSNIKTLDDLFPLRSMVFMLGTPYYGCTGEVQDSGDVITEGRIRVIFSIPCEPNLDALIQNQHKYSIK
  YNPGYVLASRLGVSGYLVSRFTGSIFIGRGSRRNPHGDHKANVGLNLKFNKKNEEVPGYTKKVGSEWMYS
  SAAEQLLAEYLERAPELFSYIAKNSQEDVFYEDDIWPGENENGAEKVQEIITWLKGHPVSTLSRSSCDLQ
  ILDAAIVEKIEEEVEKCKQRKNNKKVRVTVKPHLLYRPLEQQHGVIPDRDAEFCLFDRVVNVRENFSVPV
  GLRGTIIGIKGANREADVLFEVLFDEEFPGGLTIRCSPGRGYRLPTSALVNLSHGSRSETGNQKLTAIVK
  PQPAVHQHSSSSSVSSGHLGALNHSPQSLFVPTQVPTKDDDEFCNIWQSLQGSGKMQYFQPTIQEKGAVL
  PQEISQVNQHHKSGFNDNSVKYQQRKHDPHRKFKEECKSPKAECWSQKMSNKQPNSGIENFLASLNISKE
  NEVQSSHHGEPPSEEHLSPQSFAMGTRMLKEILKIDGSNTVDHKNEIKQIANEIPVSSNRRDEYGLPSQP
  KQNKKLASYMNKPHSANEYHNVQSMDNMCWPAPSQIPPVSTPVTELSRICSLVGMPQPDFSFLRMPQTMT
  VCQVKLSNGLLVHGPQCHSENEAKEKAALFALQQLGSLGMNFPLPSQVFANYPSAVPPGTIPPAFPPPTG
  WDHYGSNYALGAANIMPSSSHLFGSMPWGPSVPVPGKPFHHTLYSGTMPMAGGIPGGVHNQFIPLQVTKK
  RVANKKNFENKEAQSSQATPVQTSQPDSSNIVKVSPRESSSASLKSSPIAQPASSFQVETASQGHSISHH
  KSTPISSSRRKSRKLAVNFGVSKPSE
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_019001
• ORF Size: 5118 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.

Reference Data

• RefSeq: NM_019001.1, NM_019001.2, NM_019001.3, NM_019001.4, NP_061874.2, NP_061874.3
• RefSeq Size: 10123
• RefSeq ORF: 5121
• Locus ID: 54464
• Protein Pathways: RNA degradation
• MW: 193.9 kDa
• Gene Summary: This gene encodes a member of the 5'-3' exonuclease family. The encoded protein may be involved in replication-dependent histone mRNA degradation, and interacts directly with the enhancer of mRNA-decapping protein 4. In addition to mRNA metabolism, a similar protein in yeast has been implicated in a variety of nuclear and cytoplasmic functions, including homologous recombination, meiosis, telomere maintenance, and microtubule assembly. Mutations in this gene are associated with osteosarcoma, suggesting that the encoded protein may also play a role in bone formation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2013]