NAGS (NM_153006) Human Tagged ORF Clone

CAT#: RC221282

NAGS (Myc-DDK-tagged)-Human N-acetylglutamate synthase (NAGS)

Product Images

Western blot validation of overexpression lysate (Cat# LY407192) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221282 using transfection reagent MegaTran 2.0 (Cat# TT210002).

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  Coomassie blue staining of purified NAGS protein (Cat# TP321282). The protein was produced from HEK293T cells transfected with NAGS cDNA clone (Cat# RC221282) using MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: NAGS
• Synonyms: AGAS; ARGA
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221282 representing NM_153006
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGCGACGGCGCTGATGGCTGTGGTTCTGCGGGCAGCTGCTGTAGCCCCGAGGCTGAGAGGCCGGGGAG
  GCACTGGGGGCGCCCGAAGGCTGAGCTGTGGCGCGCGGCGGCGGGCGGCGAGGGGCACCAGCCCGGGGCG
  CCGGCTCAGCACCGCCTGGTCGCAGCCCCAGCCCCCGCCCGAGGAGTACGCGGGCGCGGACGACGTCTCC
  CAGTCGCCCGTCGCCGAGGAGCCGTCGTGGGTGCCGAGTCCCAGGCCCCCGGTGCCCCACGAGTCCCCAG
  AGCCTCCTTCGGGCCGCTCGCTGGTGCAGCGGGACATCCAGGCCTTCCTGAACCAGTGCGGGGCCAGCCC
  TGGGGAGGCGCGCCACTGGCTCACGCAGTTCCAGACCTGCCATCACTCCGCGGACAAGCCCTTCGCCGTC
  ATCGAGGTGGACGAGGAGGTGCTCAAGTGCCAGCAGGGCGTATCCAGTCTGGCCTTTGCCCTGGCCTTCT
  TGCAGCGCATGGACATGAAGCCGCTGGTGGTCCTGGGGCTGCCGGCCCCTACGGCTCCCTCGGGCTGTCT
  TTCCTTCTGGGAGGCCAAGGCGCAGCTGGCCAAGAGCTGCAAGGTGCTGGTAGACGCGCTTCGACACAAC
  GCCGCCGCTGCTGTGCCATTTTTTGGCGGCGGGTCTGTGCTACGCGCTGCCGAGCCGGCTCCCCATGCCA
  GCTACGGCGGCATCGTCTCGGTGGAGACAGACCTGCTGCAGTGGTGCCTGGAGTCGGGCAGCATCCCCAT
  CCTGTGCCCCATCGGGGAGACGGCCGCGCGCCGCTCCGTGCTTCTCGACTCCCTGGAGGTGACCGCGTCG
  CTGGCCAAGGCGCTGCGGCCCACCAAAATCATCTTCCTCAATAACACAGGCGGCCTGCGCGACAGCAGTC
  ATAAGGTCCTGAGTAACGTGAACCTGCCCGCCGACCTGGACCTGGTGTGCAACGCCGAGTGGGTGAGCAC
  AAAAGAACGGCAGCAGATGCGGCTCATCGTGGACGTGCTCAGCCGCCTGCCCCACCACTCCTCGGCCGTC
  ATCACCGCCGCTAGCACGCTGCTCACTGAGCTCTTTAGCAACAAGGGGTCCGGGACCCTGTTCAAGAACG
  CCGAGCGAATGCTACGGGTGCGCAGCCTGGACAAGCTGGACCAGGGCCGTCTAGTGGACCTGGTCAACGC
  CAGCTTCGGCAAGAAGCTCAGGGACGACTACCTGGCCTCGCTGCGCCCGCGGCTGCACTCCATCTACGTC
  TCCGAGGGGTACAACGCCGCCGCCATTCTGACCATGGAGCCCGTCCTGGGGGGCACCCCGTACCTGGACA
  AATTTGTGGTGAGCTCCAGCCGCCAGGGCCAAGGCTCCGGCCAGATGCTGTGGGAGTGCCTGCGGCGGGA
  CCTTCAGACACTTTTCTGGCGCTCCCGGGTCACCAACCCCATCAATCCCTGGTACTTCAAACACAGTGAT
  GGCAGCTTCTCCAACAAGCAGTGGATCTTCTTCTGGTTTGGCCTGGCTGATATCCGGGACTCCTATGAGT
  TGGTCAACCACGCCAAGGGACTGCCAGACTCCTTTCACAAGCCAGCTTCTGACCCAGGCAGC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221282 representing NM_153006
  Red=Cloning site Green=Tags(s)
  
  MATALMAVVLRAAAVAPRLRGRGGTGGARRLSCGARRRAARGTSPGRRLSTAWSQPQPPPEEYAGADDVS
  QSPVAEEPSWVPSPRPPVPHESPEPPSGRSLVQRDIQAFLNQCGASPGEARHWLTQFQTCHHSADKPFAV
  IEVDEEVLKCQQGVSSLAFALAFLQRMDMKPLVVLGLPAPTAPSGCLSFWEAKAQLAKSCKVLVDALRHN
  AAAAVPFFGGGSVLRAAEPAPHASYGGIVSVETDLLQWCLESGSIPILCPIGETAARRSVLLDSLEVTAS
  LAKALRPTKIIFLNNTGGLRDSSHKVLSNVNLPADLDLVCNAEWVSTKERQQMRLIVDVLSRLPHHSSAV
  ITAASTLLTELFSNKGSGTLFKNAERMLRVRSLDKLDQGRLVDLVNASFGKKLRDDYLASLRPRLHSIYV
  SEGYNAAAILTMEPVLGGTPYLDKFVVSSSRQGQGSGQMLWECLRRDLQTLFWRSRVTNPINPWYFKHSD
  GSFSNKQWIFFWFGLADIRDSYELVNHAKGLPDSFHKPASDPGS
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_153006
• ORF Size: 1602 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_153006.1, NM_153006.2, NP_694551.1
• RefSeq Size: 2086
• RefSeq ORF: 1605
• Locus ID: 162417
• Protein Pathways: Arginine and proline metabolism, Metabolic pathways
• MW: 58 kDa
• Gene Summary: The N-acetylglutamate synthase gene encodes a mitochondrial enzyme that catalyzes the formation of N-acetylglutamate (NAG) from glutamate and acetyl coenzyme-A. NAG is a cofactor of carbamyl phosphate synthetase I (CPSI), the first enzyme of the urea cycle in mammals. This gene may regulate ureagenesis by altering NAG availability and, thereby, CPSI activity. Deficiencies in N-acetylglutamate synthase have been associated with hyperammonemia. [provided by RefSeq, Jul 2008]