SGLT1 (SLC5A1) (NM_000343) Human Tagged ORF Clone

CAT#: RC221312

SLC5A1 (Myc-DDK-tagged)-Human solute carrier family 5 (sodium/glucose cotransporter), member 1 (SLC5A1), transcript variant 1

Product Images

Western blot validation of overexpression lysate (Cat# LY400126) using anti-DDK antibody (Cat# TA50011-100). Left: Cell lysates from un-transfected HEK293T cells; Right: Cell lysates from HEK293T cells transfected with RC221312 using transfection reagent MegaTran 2.0 (Cat# TT210002).

Specifications

Product Data

• Type: Human Tagged ORF Clone
• Tag: Myc-DDK
• Symbol: SLC5A1
• Synonyms: D22S675; NAGT; SGLT1
• Vector: pCMV6-Entry
• E. coli Selection: Kanamycin (25 ug/mL)
• Mammalian Cell Selection: Neomycin
• Sequence Data:
  ORF Nucleotide Sequence

  >RC221312 representing NM_000343
  Red=Cloning site Blue=ORF Green=Tags(s)
  
  TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
  GCCGCGATCGCC
  
  ATGGACAGTAGCACCTGGAGCCCCAAGACCACCGCGGTCACCCGGCCTGTTGAGACCCACGAGCTCATTC
  GCAATGCAGCCGATATCTCCATCATCGTTATCTACTTCGTGGTAGTGATGGCCGTCGGACTGTGGGCTAT
  GTTTTCCACCAATCGTGGGACTGTTGGAGGCTTCTTCCTGGCAGGCCGAAGTATGGTGTGGTGGCCGATT
  GGAGCCTCCCTCTTTGCTAGTAACATTGGAAGTGGCCACTTTGTGGGGCTGGCCGGGACTGGGGCAGCTT
  CAGGCATCGCCATTGGAGGCTTTGAATGGAATGCCCTGGTTTTGGTGGTTGTGCTGGGCTGGCTGTTTGT
  CCCCATCTATATTAAGGCTGGGGTGGTGACAATGCCAGAGTACCTGAGGAAGCGGTTTGGAGGCCAGCGG
  ATCCAGGTCTACCTTTCCCTTCTGTCCCTGCTGCTCTACATTTTCACCAAGATCTCGGCAGACATCTTCT
  CGGGGGCCATATTCATCAATCTGGCCTTAGGCCTGAATCTGTATTTAGCCATCTTTCTCTTATTGGCAAT
  CACTGCCCTTTACACAATTACAGGGGGCCTGGCGGCGGTGATTTACACGGACACCTTGCAGACGGTGATC
  ATGCTGGTGGGGTCTTTAATCCTGACTGGGTTTGCTTTTCACGAAGTGGGAGGCTATGACGCCTTCATGG
  AAAAGTACATGAAAGCCATTCCAACCATAGTGTCTGATGGCAACACCACCTTTCAGGAAAAATGCTACAC
  TCCAAGGGCCGACTCCTTCCACATCTTCCGAGATCCCCTCACGGGAGACCTCCCATGGCCTGGGTTCATC
  TTTGGGATGTCCATCCTTACCTTGTGGTACTGGTGCACAGATCAGGTCATTGTGCAGCGCTGCCTCTCAG
  CCAAGAATATGTCTCACGTGAAGGGTGGCTGCATCCTGTGTGGGTATCTAAAGCTGATGCCCATGTTCAT
  CATGGTGATGCCAGGAATGATCAGCCGCATTCTGTACACAGAAAAAATTGCCTGTGTCGTCCCTTCAGAA
  TGTGAGAAATATTGCGGTACCAAGGTTGGCTGTACCAACATCGCCTATCCAACCTTAGTGGTGGAGCTCA
  TGCCCAATGGACTGCGAGGCCTGATGCTATCAGTCATGCTGGCCTCCCTCATGAGCTCCCTGACCTCCAT
  CTTCAACAGCGCCAGCACCCTCTTCACCATGGACATCTACGCCAAGGTCCGCAAGAGAGCATCTGAGAAA
  GAGCTCATGATTGCCGGAAGGTTGTTTATCCTGGTGCTGATTGGCATCAGCATCGCCTGGGTGCCCATTG
  TGCAGTCAGCACAAAGTGGGCAACTCTTCGATTACATCCAGTCCATCACCAGTTACTTGGGACCACCCAT
  TGCGGCTGTCTTCCTGCTTGCTATTTTCTGGAAGAGAGTCAATGAGCCAGGAGCCTTTTGGGGACTGATC
  CTAGGACTTCTGATTGGGATTTCACGTATGATTACTGAGTTTGCTTATGGAACCGGGAGCTGCATGGAGC
  CCAGCAACTGTCCCACGATTATCTGTGGGGTGCACTACTTGTACTTTGCCATTATCCTCTTCGCCATTTC
  TTTCATCACCATCGTGGTCATCTCCCTCCTCACCAAACCCATTCCGGATGTGCATCTCTACCGTCTGTGT
  TGGAGCCTGCGCAACAGCAAAGAGGAGCGTATTGACCTGGATGCGGAAGAGGAGAACATCCAAGAAGGCC
  CTAAGGAGACCATTGAAATAGAAACACAAGTTCCTGAGAAGAAAAAAGGAATCTTCAGGAGAGCCTATGA
  CCTATTTTGTGGGCTAGAGCAGCACGGTGCACCCAAGATGACTGAGGAAGAGGAGAAAGCCATGAAGATG
  AAGATGACGGACACCTCTGAGAAGCCTTTGTGGAGGACAGTGTTGAACGTCAATGGCATCATCCTGGTGA
  CCGTGGCTGTCTTTTGCCATGCATATTTTGCC
  
  ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
  ACAAGGATGACGACGATAAGGTTTAA
  Protein Sequence

  >RC221312 representing NM_000343
  Red=Cloning site Green=Tags(s)
  
  MDSSTWSPKTTAVTRPVETHELIRNAADISIIVIYFVVVMAVGLWAMFSTNRGTVGGFFLAGRSMVWWPI
  GASLFASNIGSGHFVGLAGTGAASGIAIGGFEWNALVLVVVLGWLFVPIYIKAGVVTMPEYLRKRFGGQR
  IQVYLSLLSLLLYIFTKISADIFSGAIFINLALGLNLYLAIFLLLAITALYTITGGLAAVIYTDTLQTVI
  MLVGSLILTGFAFHEVGGYDAFMEKYMKAIPTIVSDGNTTFQEKCYTPRADSFHIFRDPLTGDLPWPGFI
  FGMSILTLWYWCTDQVIVQRCLSAKNMSHVKGGCILCGYLKLMPMFIMVMPGMISRILYTEKIACVVPSE
  CEKYCGTKVGCTNIAYPTLVVELMPNGLRGLMLSVMLASLMSSLTSIFNSASTLFTMDIYAKVRKRASEK
  ELMIAGRLFILVLIGISIAWVPIVQSAQSGQLFDYIQSITSYLGPPIAAVFLLAIFWKRVNEPGAFWGLI
  LGLLIGISRMITEFAYGTGSCMEPSNCPTIICGVHYLYFAIILFAISFITIVVISLLTKPIPDVHLYRLC
  WSLRNSKEERIDLDAEEENIQEGPKETIEIETQVPEKKKGIFRRAYDLFCGLEQHGAPKMTEEEEKAMKM
  KMTDTSEKPLWRTVLNVNGIILVTVAVFCHAYFA
  
  TRTRPLEQKLISEEDLAANDILDYKDDDDKV
• Restriction Sites: SgfI-MluI Cloning Scheme for this gene Plasmid Map
• ACCN: NM_000343
• ORF Size: 1992 bp
• OTI Disclaimer: The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
• OTI Annotation: This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
• Product Components: The ORF clone is ion-exchange column purified, transfection-ready dried plasmid DNA, and shipped with 2 vector sequencing primers.
• Reconstitution: 1. Centrifuge at 5,000xg for 5min.
  2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
  3. Close the tube and incubate for 10 minutes at room temperature.
  4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
  5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.

Reference Data

• RefSeq: NM_000343.1, NM_000343.2, NM_000343.3, NP_000334.1
• RefSeq Size: 2449 bp
• RefSeq ORF: 1995 bp
• Locus ID: 6523
• Domains: SSF
• Protein Families: Druggable Genome, Transmembrane
• MW: 73.3 kDa
• Gene Summary: This gene encodes a member of the sodium-dependent glucose transporter (SGLT) family. The encoded integral membrane protein is the primary mediator of dietary glucose and galactose uptake from the intestinal lumen. Mutations in this gene have been associated with glucose-galactose malabsorption. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jan 2012]